Difference between revisions of "Team:SDU CHINA/basic parts"
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<div class="content">Both hTERT and survivin are tumor-specific promoter, so the parameter of starting efficiency after transfection is most important. | <div class="content">Both hTERT and survivin are tumor-specific promoter, so the parameter of starting efficiency after transfection is most important. | ||
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<img src="https://static.igem.org/mediawiki/2017/7/7a/Basic1.png" style="width:60%"></div> | <img src="https://static.igem.org/mediawiki/2017/7/7a/Basic1.png" style="width:60%"></div> | ||
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<div class="img"><strong>Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></div> | <div class="img"><strong>Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></div> | ||
<div class="img"><strong>TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.</strong></div> | <div class="img"><strong>TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.</strong></div> | ||
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<img src="https://static.igem.org/mediawiki/2017/d/da/Basic2.png" style="width:40%"></div> | <img src="https://static.igem.org/mediawiki/2017/d/da/Basic2.png" style="width:40%"></div> | ||
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<div class="img"><strong>Figure 1: The express efficiency of hTERT promoter in A549 cell line.</strong></div> | <div class="img"><strong>Figure 1: The express efficiency of hTERT promoter in A549 cell line.</strong></div> | ||
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<img src="https://static.igem.org/mediawiki/2017/4/45/Basic3.png" style="width:60%"> </div> | <img src="https://static.igem.org/mediawiki/2017/4/45/Basic3.png" style="width:60%"> </div> | ||
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<div class="img"><strong>Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></div> | <div class="img"><strong>Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></div> | ||
<div class="img"><strong>TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.</strong></div> | <div class="img"><strong>TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.</strong></div> | ||
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<img src="https://static.igem.org/mediawiki/2017/6/6f/Basic4.png" style="width:40%"></div> | <img src="https://static.igem.org/mediawiki/2017/6/6f/Basic4.png" style="width:40%"></div> | ||
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<div class="img"><strong>Figure 2 :The express efficiency of survivin promoter in A549 cell line</strong></span>.</div> | <div class="img"><strong>Figure 2 :The express efficiency of survivin promoter in A549 cell line</strong></span>.</div> | ||
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| − | <h3 align="center" class=" | + | </span> |
| + | <h3 align="center" class="STYLE15">For more details, please refer to the <a href="https://2017.igem.org/Team:SDU_CHINA/Measurement">results</a>.</h3> | ||
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Revision as of 14:16, 30 October 2017
Table of the Basic Parts we submitted to the BioBrick registry
Part Number |
Name |
Type |
Length |
Description |
BBa_K2321000 |
hTERT promoter |
Regulatory |
809 |
tumor-specific promoter |
BBa_K2321001 |
Survivin promoter |
Regulatory |
1024 |
tumor-specific promoter |
BBa_K2321002 |
Luciferase |
Reporter |
1653 |
firefly luciferase reporter |
Both hTERT and survivin are tumor-specific promoter, so the parameter of starting efficiency after transfection is most important.
In order to explore the specificity and sensitivity of starting efficiency for each promoter, they were respectively inserted into double luciferase reporter vector. Then we selected two kinds of NSCLC cell lines, including A549， and measured the relative luciferase expression activity of the plasmids in different cell lines and different time points.
Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on
TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.
Figure 1: The express efficiency of hTERT promoter in A549 cell line.
Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on
TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.
Figure 2 :The express efficiency of survivin promoter in A549 cell line.
For more details, please refer to the results.
