Difference between revisions of "Team:Oxford/Parts"

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         <td>mCherry - TEV protease (non-cleaving)</td>
 
         <td>mCherry - TEV protease (non-cleaving)</td>
 
         <td>Doesn’t self-cleave</td>
 
         <td>Doesn’t self-cleave</td>
−
         <td>?</td>
+
         <td>BBa_J06504; BBa_K1319004</td>
−
         <td>?</td>
+
         <td>Linker is Nd2 from https://www.hindawi.com/journals/bmri/2009/591923/tab2/</td>
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
Line 31: Line 31:
 
     <td>Modified TetR with TEV cleavage site - CFP</td>
 
     <td>Modified TetR with TEV cleavage site - CFP</td>
 
     <td>TEV cleavage site in linker between DNA binding and dimerisation domains</td>
 
     <td>TEV cleavage site in linker between DNA binding and dimerisation domains</td>
−
     <td>BBa_K106669; BBa_K???????</td>
+
     <td>BBa_K106669</td>
 
     <td>TEV cleavage site from Merck; CFP from AddGene</td>
 
     <td>TEV cleavage site from Merck; CFP from AddGene</td>
 
       </tr>
 
       </tr>
Line 39: Line 39:
 
      <td>TetR - CFP</td>
 
      <td>TetR - CFP</td>
 
      <td>Normal TetR Class B</td>
 
      <td>Normal TetR Class B</td>
−
      <td>BBa_K106669; BBa_K???????</td>
+
      <td>BBa_K106669</td>
 
      <td>CFP from AddGene</td>
 
      <td>CFP from AddGene</td>
 
  </tr>
 
  </tr>
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      <td>Tet promoter - RBS - YFP</td>
 
      <td>Tet promoter - RBS - YFP</td>
 
      <td>DNA part, contains promoter and RBS to give signal of YFP</td>
 
      <td>DNA part, contains promoter and RBS to give signal of YFP</td>
−
      <td>BBa_R0040</td>
+
      <td>BBa_R0040; BBa_B0030</td>
−
      <td>?</td>
+
      <td>eYFP from AddGene</td>
 
  </tr>
 
  </tr>
 
     </tbody>
 
     </tbody>
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         <td>N-SpyCatcher - OmpA - sfGFP</td>
 
         <td>N-SpyCatcher - OmpA - sfGFP</td>
 
         <td>SpyCatcher is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm</td>
 
         <td>SpyCatcher is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm</td>
−
         <td>?</td>
+
         <td>n/a</td>
 
         <td>Alves et al, 2016 AddGene</td>
 
         <td>Alves et al, 2016 AddGene</td>
 
       </tr>
 
       </tr>
Line 80: Line 80:
 
     <td>OmpA - C-SpyCatcher - sfGFP</td>
 
     <td>OmpA - C-SpyCatcher - sfGFP</td>
 
     <td>SpyCatcher is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm</td>
 
     <td>SpyCatcher is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm</td>
−
     <td>?</td>
+
     <td>n/a</td>
 
     <td>Alves et al, 2016</td>
 
     <td>Alves et al, 2016</td>
 
       </tr>
 
       </tr>
Line 96: Line 96:
 
      <td>SpyTag - sfGFP</td>
 
      <td>SpyTag - sfGFP</td>
 
      <td>Control to check localisation to OMVs</td>
 
      <td>Control to check localisation to OMVs</td>
−
      <td>?</td>
+
      <td>n/a</td>
 
      <td>Alves et al, 2016</td>
 
      <td>Alves et al, 2016</td>
 
  </tr>
 
  </tr>
Line 104: Line 104:
 
      <td>sfGFP - N-terminal split TEV Protease - Inhibited Coiled Coil</td>
 
      <td>sfGFP - N-terminal split TEV Protease - Inhibited Coiled Coil</td>
 
      <td>Inhibited coiled coil contains a TEV cleavage site between the two helicies, leaving the acid coiled coil attached to the N-terminal split protease</td>
 
      <td>Inhibited coiled coil contains a TEV cleavage site between the two helicies, leaving the acid coiled coil attached to the N-terminal split protease</td>
−
      <td>?</td>
+
      <td>n/a</td>
 
      <td>Alves et al, 2016 Split TEV paper</td>
 
      <td>Alves et al, 2016 Split TEV paper</td>
 
  </tr>
 
  </tr>
Line 112: Line 112:
 
      <td>mCherry - Inhibited Coiled Coil - C-terminal Split TEV Protease</td>
 
      <td>mCherry - Inhibited Coiled Coil - C-terminal Split TEV Protease</td>
 
      <td>Inhibited coiled coil contains a TEV protease cleavage site. This will result in the cleavage of the mCherry - Acid Coil being cleaved from the Base Coil - C Terminal Split TEV Protease.</td>
 
      <td>Inhibited coiled coil contains a TEV protease cleavage site. This will result in the cleavage of the mCherry - Acid Coil being cleaved from the Base Coil - C Terminal Split TEV Protease.</td>
−
      <td>?</td>
+
      <td>n/a</td>
 
      <td>Alves et al, 2016 Split TEV paper</td>
 
      <td>Alves et al, 2016 Split TEV paper</td>
 
  </tr>
 
  </tr>
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<div class="column half_size">
 
<div class="column half_size">
−
 
−
<h5>Inspiration</h5>
 
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
−
 
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
−
</ul>
 
−
</div>
 
−
 
  
 
</html>
 
</html>

Revision as of 16:11, 30 October 2017

Parts

Parts for the Cell-Free System

Registry Number Code used in Lab Brief Description Key Details Biobricks used Other sequence sources
BBa_K2450101 C100 mCherry - TEV protease (non-cleaving) Doesn’t self-cleave BBa_J06504; BBa_K1319004 Linker is Nd2 from https://www.hindawi.com/journals/bmri/2009/591923/tab2/
BBa_K2450201 C200 Modified TetR with TEV cleavage site - CFP TEV cleavage site in linker between DNA binding and dimerisation domains BBa_K106669 TEV cleavage site from Merck; CFP from AddGene
BBa_K2450251 C250 TetR - CFP Normal TetR Class B BBa_K106669 CFP from AddGene
BBa_K2450301 C300 Tet promoter - RBS - YFP DNA part, contains promoter and RBS to give signal of YFP BBa_R0040; BBa_B0030 eYFP from AddGene

Parts for the OMV System

Registry Number Code used in Lab Brief Description Key Details Biobricks used Other sequence sources
BBa_K2450401 V100 N-SpyCatcher - OmpA - sfGFP SpyCatcher is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm n/a Alves et al, 2016 AddGene
BBa_K2450451 v150 OmpA - C-SpyCatcher - sfGFP SpyCatcher is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm n/a Alves et al, 2016
BBa_K2450501 v200 SpyTag - sfGFP - Quencher TEV cleavage site between sfGFP and the quenching peptide BBa_K1319014 Alves et al, 2016
BBa_K2450502 V200noQ SpyTag - sfGFP Control to check localisation to OMVs n/a Alves et al, 2016
BBa_K2450701 V500 sfGFP - N-terminal split TEV Protease - Inhibited Coiled Coil Inhibited coiled coil contains a TEV cleavage site between the two helicies, leaving the acid coiled coil attached to the N-terminal split protease n/a Alves et al, 2016 Split TEV paper
BBa_K2450702 V600 mCherry - Inhibited Coiled Coil - C-terminal Split TEV Protease Inhibited coiled coil contains a TEV protease cleavage site. This will result in the cleavage of the mCherry - Acid Coil being cleaved from the Base Coil - C Terminal Split TEV Protease. n/a Alves et al, 2016 Split TEV paper