Difference between revisions of "Team:Chalmers-Gothenburg/Notebook/protocols"

 
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.header-text {
 
.header-text {
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               <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/InterLab">Interlab study</a>
 
               <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/InterLab">Interlab study</a>
 
               <hr>
 
               <hr>
−
               <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/Achievements/medal">Medals</a>
+
               <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/Achievements/medal">Accomplishments</a>
 
             </div>
 
             </div>
 
           </li>
 
           </li>
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     <div class="wrap"> <!--To limit the width of the site-->
 
     <div class="wrap"> <!--To limit the width of the site-->
  
−
         
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         <div class="header_test">
−
         <div class="header_Team_collaborations">
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  <!-- Banner -->
−
    <div class="header-text">Notebook</div>
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    <div id="banner">
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    <div class="header-subtitle">Protocols</div>
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            <h3>Protocols</h3>
−
    </div>
+
            </div>
 +
        </div>
 
        
 
        
 
       <div class="wrap-content">
 
       <div class="wrap-content">
 
           <h4 class="subtitle">Introduction</h4>
 
           <h4 class="subtitle">Introduction</h4>
 
             <p class="text">
 
             <p class="text">
−
Several protocols for specific procedures have been followed while working in the wet lab. These include protocols for PCR, purifications, digestions, assemblies as well as working with Saccharomyces cerevisiae and Escherichia coli. See the exact procedures in the <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/Notebook">lab journal</a>.  
+
Several protocols for specific procedures have been followed while working in the wet lab. These include protocols for PCR, purifications, digestions, assemblies as well as working with <i>Saccharomyces cerevisiae</i> and <i>Escherichia coli</i>. See the exact procedures under <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/Notebook">Notebook, lab journal</a>.  
 
           </p>
 
           </p>
 
          
 
          
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<div style="padding-left: 25px">
 
<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
−
           <a href="https://static.igem.org/mediawiki/2016/e/e9/T--Chalmers_Gothenburg--Protocols_DreamTaq_PCR.pdf">Thermo Scientific DreamTaq DNA Polymerase</a><br>
+
           <a href="https://static.igem.org/mediawiki/2016/e/e9/T--Chalmers_Gothenburg--Protocols_DreamTaq_PCR.pdf" target="blank">Thermo Scientific DreamTaq DNA Polymerase</a><br>
  
−
           <a href="https://static.igem.org/mediawiki/2016/c/c9/T--Chalmers_Gothenburg--Protocols_Phusion_PCR.pdf">Thermo Scientific Phusion High-Fidelity DNA Polymerase</a><br>
+
           <a href="https://static.igem.org/mediawiki/2016/c/c9/T--Chalmers_Gothenburg--Protocols_Phusion_PCR.pdf" target="blank">Thermo Scientific Phusion High-Fidelity DNA Polymerase</a><br>
  
−
           <a href="https://static.igem.org/mediawiki/2016/a/a3/T--Chalmers_Gothenburg--Protocols_PrimeStar_PCR.pdf">PrimeSTAR HS DNA Polymerase</a><br>
+
           <a href="https://static.igem.org/mediawiki/2017/c/c8/T--Chalmers-Gothenburg--Takara_PrimeSTAR_HS_DNA_Polymerase.pdf" target="blank">PrimeSTAR HS DNA Polymerase</a><br>
  
−
           <a href="https://static.igem.org/mediawiki/2017/3/36/T--Chalmers-Gothenburg--Fusion_PCR.pdf">Fusion PCR</a>
+
           <a href="https://static.igem.org/mediawiki/2017/3/36/T--Chalmers-Gothenburg--Fusion_PCR.pdf" target="blank">Fusion PCR</a>
 
         </p>
 
         </p>
 
</div>
 
</div>
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<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
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           <a href="https://static.igem.org/mediawiki/2017/8/81/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_PCR_Purification_Kit.pdf">Thermo Scientific GeneJET PCR Purification Kit</a><br>
+
           <a href="https://static.igem.org/mediawiki/2017/8/81/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_PCR_Purification_Kit.pdf" target="blank">Thermo Scientific GeneJET PCR Purification Kit</a><br>
  
−
           <a href="https://static.igem.org/mediawiki/2017/8/89/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Gel_Extraction_Kit.pdf">Thermo Scientific GeneJET Gel Extraction Kit</a>
+
           <a href="https://static.igem.org/mediawiki/2017/8/89/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Gel_Extraction_Kit.pdf" target="blank">Thermo Scientific GeneJET Gel Extraction Kit</a>
 
         </p>
 
         </p>
 
</div>
 
</div>
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<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
−
           <a href="https://static.igem.org/mediawiki/2017/e/e9/T--Chalmers-Gothenburg--FastDigest_Restriction_Enzymes.pdf">FastDigest Restriction Enzymes</a><br>
+
           <a href="https://static.igem.org/mediawiki/2017/e/e9/T--Chalmers-Gothenburg--FastDigest_Restriction_Enzymes.pdf" target="blank">FastDigest Restriction Enzymes</a><br>
−
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+
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−
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+
           <a href="https://static.igem.org/mediawiki/2016/1/1d/T--Chalmers_Gothenburg--Protocols_general_DNA_insert_ligation.pdf" target="blank">DNA Insert Ligation into Vector DNA</a>
 
         </p>
 
         </p>
 
</div>
 
</div>
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<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
−
           <a href="https://static.igem.org/mediawiki/2017/7/74/T--Chalmers-Gothenburg--Yeast_Transformation_Chemical.pdf">Transformation (Chemical)</a><br>
+
           <a href="https://static.igem.org/mediawiki/2017/7/74/T--Chalmers-Gothenburg--Yeast_Transformation_Chemical.pdf" target="blank">Transformation (Chemical)</a><br>
  
−
           <a href="https://static.igem.org/mediawiki/2016/d/de/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_yeast_electroporation.pdf">Transformation (Electroporation)</a><br>
+
           <a href="https://static.igem.org/mediawiki/2016/d/de/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_yeast_electroporation.pdf" target="blank">Transformation (Electroporation)</a><br>
  
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           <a href="https://static.igem.org/mediawiki/2017/0/09/T--Chalmers-Gothenburg--Yeast_Transformation-Quick_Version.pdf">Transformation (Quick version)</a><br>
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           <a href="https://static.igem.org/mediawiki/2017/0/09/T--Chalmers-Gothenburg--Yeast_Transformation-Quick_Version.pdf" target="blank">Transformation (Quick version)</a><br>
  
−
           <a href="https://static.igem.org/mediawiki/2016/e/e2/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_quick_genomic_extraction.pdf">Isolation of genomic DNA</a>
+
           <a href="https://static.igem.org/mediawiki/2016/e/e2/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_quick_genomic_extraction.pdf" target="blank">Isolation of genomic DNA</a>
 
         </p>
 
         </p>
 
</div>
 
</div>
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<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
−
           <a href="https://static.igem.org/mediawiki/2016/f/f6/T--Chalmers_Gothenburg--Protocols_E_coli_transformation.pdf">Transformation (Heat shock)</a><br>
+
           <a href="https://static.igem.org/mediawiki/2016/f/f6/T--Chalmers_Gothenburg--Protocols_E_coli_transformation.pdf" target="blank">Transformation (Heat shock)</a><br>
−
           <a href="https://static.igem.org/mediawiki/2017/b/b1/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Plasmid_Miniprep_Kit.pdf">Thermo Scientific GeneJET Plasmid Miniprep Kit</a>
+
           <a href="https://static.igem.org/mediawiki/2017/b/b1/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Plasmid_Miniprep_Kit.pdf" target="blank">Thermo Scientific GeneJET Plasmid Miniprep Kit</a>
 
         </p>
 
         </p>
 
</div>
 
</div>
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<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
−
           <a href="https://static.igem.org/mediawiki/2016/c/c1/T--Chalmers_Gothenburg--Protocols_biobrick_assembly.pdf">BioBricks Assembly Protocol</a>
+
           <a href="https://static.igem.org/mediawiki/2016/c/c1/T--Chalmers_Gothenburg--Protocols_biobrick_assembly.pdf" target="blank">BioBricks Assembly Protocol</a>
 
         </p>
 
         </p>
 
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         <p class="text">
 
         <p class="text">
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           <a href="https://static.igem.org/mediawiki/2017/b/b4/T--Chalmers-Gothenburg--Interlab_study.pdf">Interlab study</a>
+
           <a href="https://static.igem.org/mediawiki/2017/b/b4/T--Chalmers-Gothenburg--Interlab_study.pdf" target="blank">Interlab study</a>
 
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Latest revision as of 17:39, 30 October 2017

Chalmers Gothenburg iGEM 2017

Introduction

Several protocols for specific procedures have been followed while working in the wet lab. These include protocols for PCR, purifications, digestions, assemblies as well as working with Saccharomyces cerevisiae and Escherichia coli. See the exact procedures under Notebook, lab journal.

Protocols

Protocols for PCR

Protocols for purification

Protocols for digestion and assembly

Protocols for Saccharomyces cerevisiae

Protocols for Escherichia coli

Protocols for Biobricks

Protocols for Interlab study