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<center><table><tr><td><img src="https://2017.igem.org/File:T--IISER-Mohali-INDIA--GD1.png" alt="Figure1. Genetic circuit for detection of salicylate" width="100%" height="100%"></td></tr></table></center> | <center><table><tr><td><img src="https://2017.igem.org/File:T--IISER-Mohali-INDIA--GD1.png" alt="Figure1. Genetic circuit for detection of salicylate" width="100%" height="100%"></td></tr></table></center> | ||
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− | <section><h3>To clone three modules in the <i>E.coli< | + | <section><h3>To clone three modules in the <i>E.coli<i>, they should have different copy number plasmids. The module 1 will be active even in the absence of pollutant. So, we decided to put it on low copy number plasmid. The module 2 will get activated only after pollutant is present. So, we decided to put it on high copy nymber plasmid for immediate detection. The module 3 is already present in the <i>E.coli<i> chromosome.</h3><br/> |
<h3>Module 1 :</h3> | <h3>Module 1 :</h3> | ||
<h4>It comprises two constructs :</h4> | <h4>It comprises two constructs :</h4> |
Revision as of 23:40, 30 October 2017