Difference between revisions of "Team:Cadets2Vets/Safety"

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{{Cadets2Vets}}
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{{Team:Cadets2Vets/SideMenu}}
  
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Home
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Safety - Home</title>
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</title>
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<span id="element-a1a56421418f7ce" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
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function init(){
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<span id="element-5efad87d43e4352" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
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<div class='wrapperer'>
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</a>
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<a class="removable-parent" href="description.html" data-link-type="EXISTING"  target="_self" >
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</ul>
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<span id="element-e6e2afb357139e4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Description</span>
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<li class="removable-parent">
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<a class="removable-parent" href="design.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-c6287513fca8901"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
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<li class="removable-parent">
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<a class="removable-parent" href="experiments.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-f4b485a3e8b3825"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
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<span id="element-603d2303312c4bd" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook</span>
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<li class="removable-parent">
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<a class="removable-parent" href="notebook-august.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-e39fe0beffa33cd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook August</span>
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</a>
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<li class="removable-parent">
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<a class="removable-parent" href="notebook-july.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-09ca318ecaf1b2f" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook July</span>
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<li class="removable-parent">
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<a class="removable-parent" href="notebook-june.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-367041e6445b247"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook June</span>
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<li class="removable-parent">
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<a class="removable-parent" href="public-engagement.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-307cb61a9a844e1"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
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<li class="removable-parent">
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<a class="removable-parent" href="safety.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-fa38138e1dd4fce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
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<li class="removable-parent">
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<a class="removable-parent" href="team.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-8308751370782bc"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
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<li class="removable-parent">
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<a class="removable-parent" href="basic-parts.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-78fbd0423085ce2"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
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<li class="removable-parent">
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<a class="removable-parent" href="composite-parts.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-24ea54f35798e9a"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Composite Parts</span>
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<li class="removable-parent">
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<a class="removable-parent" href="parts.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-8d68d4dbac38a10"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
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<a class="removable-parent" href="our-partners.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-be35f8069be1925"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Our Partners</span>
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<a class="removable-parent" href="notebook-september.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-4d7dd9f5c90ef9b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook September</span>
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<a class="removable-parent" href="environmental-project.html" data-link-type="EXISTING"  target="_self" >
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<span id="8d3cfa8c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Hardware</span>
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<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
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<li class="removable-parent">
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<a class="removable-parent" href="improve.html" data-link-type="EXISTING"  target="_self" >
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<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
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<li class="removable-parent">
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<span style="font-size: 22px;">We designed our project specifically because of the safety hazard presented by arsenic&nbsp;pollution, and&nbsp;Tacoma's reputation of being a city with a relatively high level of Arsenic contamination.
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<p class="MsoNormal"><span style="font-size:18px;"><span style="line-height:2em;"><span id="docs-internal-guid-1b7da29c-fd03-79be-db00-39ec8eeebe81"><span style="font-family: Arial; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">The experiments we have performed were done at Biosafety Level 1, meaning that the microbes we used are not known to consistently cause disease in healthy adults and present minimal potential hazard to lab researchers and the environment. </span></span></span></span><span style="font-size:18px;"><span style="line-height:2em;"><span><span style="font-family: Arial; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Our protocols require that we wear protective equipment and make sure not to wear our clothes or hairstyle in a way that it might contaminate our work. The two elements of our experiments that required the most caution were utilizing a UV light to view our circuit's GFP fluorescence and the use of arsenic experiment preparation.</span></span></span></span></p>
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<span style="font-family: arial, ArialRegular, helvetica, sans-serif;">Lab Safety Biosafety Level 1 (BSL-1)
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<span style="font-family: arial, ArialRegular, helvetica, sans-serif; font-size: 14px;">Safety Guidelines If you work in a lab that is designated a BSL-1, the microbes there are not known to consistently cause disease in healthy adults and present minimal potential hazard to laboratory staff and the environment. An example of a microbe that is typically worked with at a BSL-1 is a nonpathogenic strain of E. coli
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<p><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">UV light exposure in our experiments comes from 3 different places:</span></span></p><ul><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The gel imager that we used to take pictures of our DNA gels and paper tickets.</span></span></li><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The handheld UV lamp we used to preview the results of our tickets before imaging.</span></span></li><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The UV LEDs that we used in our imaging chamber with the VK3 camera.</span></span></li></ul><p><span style="font-size:12px;">​To combat these issues:</span></p><ul><li style="text-align: left;"><span style="font-size:12px;"><span style="text-align: center;">T</span><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">he gel imager was enclosed in a box with internal imaging capabilities to avoid exposure to UV light. We also had an orange filter that was used to protect our eyes if we wanted to look at the gel directly.</span></span></li><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The handheld UV lamp was not enclosed in a box, so we wore a protective face mask when using it. We also made sure to point the lamp away from ourselves and our skin.</span></span></li><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The UV LEDs in the VK3 camera were assembled inside a box to limit the exposure it had on our skin. This means turning off the LEDs when we were working on the assembly and making sure the box was closed when we tested the imaging design.</span></span></li></ul><p><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">We understand the dangers of arsenic, so we always wore gloves when weighing out the chemicals and handling the arsenic solution. Any waste product or used tickets were placed in a container and disposed of through the environmental health chemical waste disposal program. </span></span></p>
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</IMG>
+
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 +
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<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 
</div>
 
</div>
 
</div>
 
</div>
−
<div class='strechH ' style='position:absolute;left:-182px;top:630px;z-index:10001;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:1601px;height:834px;overflow:hidden;background-color:rgb(0, 0, 0);background-position-x:undefined;background-position-y:undefined;background-repeat:undefined;background-attachment:undefined;border-width:0px;border-style:solid;border-color:transparent;border-color:rgb(0, 0, 0);-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:1526px;height:834px;'>
+
 +
 +
 +
 +
 +
</div>
 
</div>
 
</div>
−
<div class='ParagraphBox '  style='position:absolute;left:-28px;top:663px;z-index:10005;padding-left:14px;padding-right:15px;padding-top:5px;padding-bottom:5px;width:910px;height:739px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:rgb(0, 0, 0);-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:870px;height:739px;overflow:hidden;'>
+
−
<div class='innerText'>
+
−
<p style="margin-top: 0px; margin-left: 0px;">
+
−
<span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular; font-size: 28px;'>Laboratory Practices
+
</div>
−
</span>
+
</div>
−
</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">&nbsp;</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">
+
−
<span style="font-size: 24px;">
+
−
<span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Individuals must wash their hands: (a) after working with potentially hazardous materials, recombinant materials and animals; (b) after removing gloves; and (c) before leaving the laboratory.
+
−
</span>
+
−
</span>
+
−
</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">
+
−
<span style="font-size: 24px;">
+
−
<span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Eating, drinking, smoking, handling contact lenses and applying cosmetics are not permitted in laboratory areas.
+
−
</span>
+
−
</span>
+
−
</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">
+
−
<span style="font-size: 24px;">
+
−
<span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Mouth pipetting is prohibited; mechanical pipetting devices must be used.
+
−
</span>
+
−
</span>
+
−
</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">
+
−
<span style="font-size: 24px;">
+
−
<span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• All procedures should be performed in such a way that minimizes splashes and/or aerosols.
+
−
</span>
+
−
</span>
+
−
</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">
+
−
<span style="font-size: 24px;">
+
−
<span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Work can be performed on an open lab bench or table .
+
−
</span>
+
−
</span>
+
−
</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">
+
−
<span style="font-size: 24px;">
+
−
<span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Decontaminate all work surfaces after completion of work and after any spill or splash of potentially infectious or viable recombinant material with appropriate disinfectant. Suitable disinfectants include: 10% bleach solution, 70% ethanol solution or 2% wescodyne solution.
+
−
</span>
+
−
</span>
+
−
</p>
+
−
<p style="margin-top: 0px; margin-left: 0px;">
+
−
<span style="font-size: 24px;">
+
−
<span style='color: rgb(255, 255, 255); font-family: "Arial Black", ArialBlackRegular;'>• Decontaminate all cultures, stocks, and other potentially infectious or recombinant materials before disposal using an effective method.
+
−
</span>
+
−
</span>
+
−
</p>
+
 
</div>
 
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
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<!-- FEATURES START -->
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<div  id="vbid-5515b55f-vfaninoc"  class="master item-box  gallery-box style-5515b55f-18irqn7a    button-effects btn_hover3      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="NATIVE_ORDER"  data-styleid="style-5515b55f-18irqn7a" data-preview-styleid='style-5515b55f-18irqn7a' data-preset-type-id="FEATURES">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
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<div class="gallery-wrapper item-wrapper features-wrapper ">
 +
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<div  class="sub container style-5515b55f-18irqn7a content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-5515b55f-18irqn7a" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-5515b55f-vfaninoc" data-preset-type-id="FEATURES" data-preview-style="style-5515b55f-18irqn7a" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="matrix"
 +
data-ARRANGER_COLS="2"
 +
data-ARRANGER_ITEM_MAX_WIDTH="1000"
 +
data-ARRANGER_ITEM_MIN_WIDTH="477.4"
 +
data-ARRANGER_ITEM_RATIO="0.1"
 +
data-ARRANGER_ITEM_SPACING="10"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<!-- STRIPE HEADER -->
 +
<div class="stripe-header-wrapper">
 +
<div  id="vbid-5515b55f-wbmtlc6v"  class="stripe-header sub item-box page-box style-5515b55f-18irqn7a      " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-5515b55f-18irqn7a" data-preview-styleid='style-5515b55f-18irqn7a' data-preset-type-id="UNRESOLVED">
 +
<div class="page-wrapper item-wrapper ">
 +
<div class="item-content leaf blocks_layout page content" data-self="vbid-5515b55f-wbmtlc6v" data-preview-style="style-5515b55f-18irqn7a" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-5515b55f-wbmtlc6v" data-bgimg="">
 +
<div class="helper-div middle" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="text-side ">
 +
<div class="vertical-aligner">
 +
<div class="item-details blocks-preview-content-wrapper  blocks shrinker-parent" style="position:relative;">
 +
<div class="blocks-preview-content-holder shrinker-content">
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="blocks-preview-title-holder removable-parent order-handle">
 +
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<h2 id="vbid-5515b55f-znzls9dz" class="preview-element blocks-preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_TITLE" >Lab Practices</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 
</div>
 
</div>
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<div class='PictureBox ' itemclicktype='none' social='false' style='position:absolute;left:26px;top:349px;z-index:10006;padding-left:0px;padding-right:0px;padding-top:0px;padding-bottom:0px;width:287px;height:304px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:#000000;-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:274px;height:304px;'>
+
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+
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+
 
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</div>
 
</div>
 
</div>
 
</div>
 
</div>
−
<div class='footer' style = 'position:absolute;top:2510px;' >
+
−
<a href='/sitemap.xml'>sitemap</a>
+
<div id="items-holder-wrapper">
 +
<div id="items-holder">
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<div class="Picture item-preview">
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<div class="vertical-aligner">
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<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
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<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
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 +
 
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<!-- MAP TEMPLATE -->
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<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
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<!-- RAW TEMPLATE -->
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<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
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</div>
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</div>
 +
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 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
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<div class="draggable-div-holder"></div>
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<div class="preview-content-holder shrinker-content">
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<!--  BY NATIVE ORDER -->
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 +
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<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
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 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-5515b55f-pmjmwiyx" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Practice #1</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-5515b55f-0vubnjas" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-5515b55f-ybjuf35l" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="MsoNormal" style="text-align: center;"><span style="font-family:montserrat;"><span style="font-size:16px;"><span style="line-height: 115%;">• Individuals must wash their hands: (a) after working with potentially hazardous materials, recombinant materials and animals; (b) after removing gloves; and (c) before leaving the laboratory.</span></span></span><span style="font-size:8.0pt;line-height:115%;font-family:
 +
&quot;Times New Roman&quot;,&quot;serif&quot;"><o:p></o:p></span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
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 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 
 +
 +
<div class="preview-form order-handle">
 +
 
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
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</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 
</div>
 
</div>
−
<script>
 
−
init()
 
−
</script>
 
 
</div>
 
</div>
−
</body>
+
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-5515b55f-nrqzdysy"  class="sub item-box  page-box style-5515b55f-18irqn7a          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-5515b55f-18irqn7a" data-preview-styleid='style-5515b55f-18irqn7a' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-5515b55f-nrqzdysy" data-preview-style="style-5515b55f-18irqn7a" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-5515b55f-nrqzdysy" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div class="column full_size">
+
<div id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<h1> Safety </h1>
+
<!-- VIDEO TEMPLATE -->
−
<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 
</div>
 
</div>
 +
  
−
<div class="column full_size">
+
</div>
−
<h5>Safe Project Design</h5>
+
</div>
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
<div class="text-side shrinker-parent">
−
<ul>
+
<div class="vertical-aligner">
−
<li>Choosing a non-pathogenic chassis</li>
+
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
<div class="draggable-div-holder"></div>
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
<div class="preview-content-holder shrinker-content">
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
−
</ul>
+
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-5515b55f-rq2iw6si" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Practice #2</h2>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
−
<div class="column half_size">
+
−
<h5>Safe Lab Work</h5>
+
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
 
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-5515b55f-kp6cm2w7" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
−
<div class="column half_size">
+
−
<h5>Safe Shipment</h5>
+
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-5515b55f-1obeib65" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="MsoNormal"><span style="font-family:montserrat;"><span style="font-size:16px;"><span style="line-height: 115%;">• Eating, drinking, smoking, handling contact lenses and applying cosmetics are not permitted in laboratory areas.</span></span></span><span style="font-size:8.0pt;line-height:115%;font-family:
 +
&quot;Times New Roman&quot;,&quot;serif&quot;"><o:p></o:p></span></p>
 +
</div>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-5515b55f-8n6bz9dn"  class="sub item-box  page-box style-5515b55f-18irqn7a          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-5515b55f-18irqn7a" data-preview-styleid='style-5515b55f-18irqn7a' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-5515b55f-8n6bz9dn" data-preview-style="style-5515b55f-18irqn7a" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-5515b55f-8n6bz9dn" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-5515b55f-gzyaegzp" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Practice #3</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-5515b55f-bfge4cb0" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-5515b55f-9tiifvjn" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="MsoNormal"><span style="font-family:montserrat;"><span style="font-size:16px;"><span style="line-height: 115%;">• Mouth pipetting is prohibited; mechanical pipetting devices must be used.</span></span></span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-5515b55f-aws8emsp"  class="sub item-box  page-box style-5515b55f-18irqn7a          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-5515b55f-18irqn7a" data-preview-styleid='style-5515b55f-18irqn7a' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-5515b55f-aws8emsp" data-preview-style="style-5515b55f-18irqn7a" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-5515b55f-aws8emsp" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-5515b55f-8aaoijyv" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Practice #4</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-5515b55f-qxehsdsv" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-5515b55f-1caxro6u" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="MsoNormal"><span style="font-family:montserrat;"><span style="font-size:16px;"><span style="line-height: 115%;">• All procedures should be performed in such a way that minimizes splashes and/or aerosols.</span></span></span><span style="font-size:8.0pt;line-height:115%;font-family:
 +
&quot;Times New Roman&quot;,&quot;serif&quot;"><o:p></o:p></span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-5515b55f-gncxh3v9"  class="sub item-box  page-box style-5515b55f-18irqn7a          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-5515b55f-18irqn7a" data-preview-styleid='style-5515b55f-18irqn7a' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-5515b55f-gncxh3v9" data-preview-style="style-5515b55f-18irqn7a" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-5515b55f-gncxh3v9" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-5515b55f-r9zworeu" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Practice #5</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-5515b55f-umokbfyb" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-5515b55f-jdbvwwb1" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="MsoNormal" style="text-align: center;"><span style="font-family:montserrat;"><span style="font-size:16px;"><span style="line-height: 115%;"></span></span></span><span style="font-family: montserrat; font-size: 16px;">• Work can be performed on an open lab bench or table</span><span style="font-family: montserrat; font-size: 16px;">.</span><span style="font-family:montserrat;"><span style="font-size:16px;"><span style="line-height: 115%;"></span></span></span><span style="font-size:8.0pt;line-height:115%;font-family:
 +
&quot;Times New Roman&quot;,&quot;serif&quot;"><o:p></o:p></span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-5515b55f-kc3zrjq7"  class="sub item-box  page-box style-5515b55f-18irqn7a          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-5515b55f-18irqn7a" data-preview-styleid='style-5515b55f-18irqn7a' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-5515b55f-kc3zrjq7" data-preview-style="style-5515b55f-18irqn7a" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-5515b55f-kc3zrjq7" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-5515b55f-tnppb1av" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Practice #6</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-5515b55f-22umctwf" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
<br class="upper-line-break" />
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<p class="MsoNormal" style="text-align: center;"><span style="font-family: montserrat; font-size: 16px;">• Decontaminate all work surfaces with appropriate disinfectant after completion of work and after any spill or splash of potentially infectious or viable recombinant material. Suitable disinfectants include: 10% bleach solution, 70% ethanol solution or 2% wescodyne solution.</span><span style="font-size:8.0pt;line-height:115%;font-family:
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 +
<p class="MsoNormal" style="text-align: center;"><span style="font-family: montserrat; font-size: 16px;">• Using an effective method, decontaminate all cultures, stocks, and other potentially infectious or recombinant materials before disposal.</span><span style="font-size:8.0pt;line-height:115%;font-family:
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<p class="MsoNormal" style="text-align: center;"><span style="line-height:1em;"><span style="font-size: 16px;">• </span><span id="docs-internal-guid-1b7da29c-f967-56fe-35c7-85a31760a18d"><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Anyone working in the lab needs to wear closed toe shoes, clothing covering down to at least the knee, and proper protective equipment as needed. </span></span></span><span style="font-size:8.0pt;line-height:115%;font-family:
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&quot;Times New Roman&quot;,&quot;serif&quot;"><o:p></o:p></span></p>
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Latest revision as of 05:47, 31 October 2017

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SAFETY



The experiments we have performed were done at Biosafety Level 1, meaning that the microbes we used are not known to consistently cause disease in healthy adults and present minimal potential hazard to lab researchers and the environment. Our protocols require that we wear protective equipment and make sure not to wear our clothes or hairstyle in a way that it might contaminate our work. The two elements of our experiments that required the most caution were utilizing a UV light to view our circuit's GFP fluorescence and the use of arsenic experiment preparation.



Overview



UV light exposure in our experiments comes from 3 different places:

  • The gel imager that we used to take pictures of our DNA gels and paper tickets.
  • The handheld UV lamp we used to preview the results of our tickets before imaging.
  • The UV LEDs that we used in our imaging chamber with the VK3 camera.

​To combat these issues:

  • The gel imager was enclosed in a box with internal imaging capabilities to avoid exposure to UV light. We also had an orange filter that was used to protect our eyes if we wanted to look at the gel directly.
  • The handheld UV lamp was not enclosed in a box, so we wore a protective face mask when using it. We also made sure to point the lamp away from ourselves and our skin.
  • The UV LEDs in the VK3 camera were assembled inside a box to limit the exposure it had on our skin. This means turning off the LEDs when we were working on the assembly and making sure the box was closed when we tested the imaging design.

We understand the dangers of arsenic, so we always wore gloves when weighing out the chemicals and handling the arsenic solution. Any waste product or used tickets were placed in a container and disposed of through the environmental health chemical waste disposal program.



Lab Practices



Practice #1





• Individuals must wash their hands: (a) after working with potentially hazardous materials, recombinant materials and animals; (b) after removing gloves; and (c) before leaving the laboratory.



Practice #2





• Eating, drinking, smoking, handling contact lenses and applying cosmetics are not permitted in laboratory areas.



Practice #3





• Mouth pipetting is prohibited; mechanical pipetting devices must be used.



Practice #4





• All procedures should be performed in such a way that minimizes splashes and/or aerosols.



Practice #5





• Work can be performed on an open lab bench or table.



Practice #6





• Decontaminate all work surfaces with appropriate disinfectant after completion of work and after any spill or splash of potentially infectious or viable recombinant material. Suitable disinfectants include: 10% bleach solution, 70% ethanol solution or 2% wescodyne solution.



Practice #7





• Using an effective method, decontaminate all cultures, stocks, and other potentially infectious or recombinant materials before disposal.



Practice #8





• Anyone working in the lab needs to wear closed toe shoes, clothing covering down to at least the knee, and proper protective equipment as needed.


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