Difference between revisions of "Team:Cadets2Vets/Safety"

The gel imager that we used to take pictures of our DNA gels and paper tickets.
The handheld UV lamp we used to preview the results of our tickets before imaging.
The UV LEDs that we used in our imaging chamber with the VK3 camera.

To combat these issues:

The gel imager was enclosed in a box with internal imaging capabilities to avoid exposure to UV light. We also had an orange filter that was used to protect our eyes if we wanted to look at the gel directly.
The handheld UV lamp was not enclosed in a box, so we wore a protective face mask when using it. We also made sure to point the lamp away from ourselves and our skin.
The UV LEDs in the VK3 camera were assembled inside a box to limit the exposure it had on our skin. This means turning off the LEDs when we were working on the assembly and making sure the box was closed when we tested the imaging design.

We understand the dangers of arsenic, so we always wore gloves when weighing out the chemicals and handling the arsenic solution. Any waste product or used tickets were placed in a container and disposed of through the environmental health chemical waste disposal program.

Difference between revisions of "Team:Cadets2Vets/Safety"

Latest revision as of 05:47, 31 October 2017

SAFETY

Overview

Lab Practices

Practice #1

Practice #2

Practice #3

Practice #4

Practice #5

Practice #6

Practice #7

Practice #8

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2304 Jefferson Ave, Tacoma, WA 98402

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+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="environmental-project.html" data-link-type="EXISTING"   target="_self" >
+			<span id="2d522622"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Environmental Project</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="hardware.html" data-link-type="EXISTING"   target="_self" >
+			<span id="8d3cfa8c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Hardware</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="demonstrate.html" data-link-type="EXISTING"   target="_self" >
+			<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="improve.html" data-link-type="EXISTING"   target="_self" >
+			<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="results.html" data-link-type="EXISTING"   target="_self" >
+			<span id="7e0ed437"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Results</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="attribution.html" data-link-type="EXISTING"   target="_self" >
+			<span id="8a69633b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Attribution</span>
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+			<a class="removable-parent" href="notebook-october.html" data-link-type="EXISTING"   target="_self" >
+			<span id="643d5085"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="silver-hp.html" data-link-type="EXISTING"   target="_self" >
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 		<li class="removable-parent">
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@@ Line 252: / Line 494: @@
 					<div id="vbid-5515b55f-09a8dz2z-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
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 		<!-- VIDEO TEMPLATE -->
@@ Line 303: / Line 545: @@
 	<div id="vbid-5515b55f-4qmf0kdx" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
-		<p class="MsoNormal"><span style="font-size:16.5pt;line-height:115%;font-family:
+		<p class="MsoNormal"><span style="font-size:18px;"><span style="line-height:2em;"><span id="docs-internal-guid-1b7da29c-fd03-79be-db00-39ec8eeebe81"><span style="font-family: Arial; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">The experiments we have performed were done at Biosafety Level 1, meaning that the microbes we used are not known to consistently cause disease in healthy adults and present minimal potential hazard to lab researchers and the environment. </span></span></span></span><span style="font-size:18px;"><span style="line-height:2em;"><span><span style="font-family: Arial; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Our protocols require that we wear protective equipment and make sure not to wear our clothes or hairstyle in a way that it might contaminate our work. The two elements of our experiments that required the most caution were utilizing a UV light to view our circuit's GFP fluorescence and the use of arsenic experiment preparation.</span></span></span></span></p>
-&quot;Georgia&quot;,&quot;serif&quot;;color:black">We designed our project specifically because of the safety hazard presented by arsenic pollution, and Tacoma's reputation of being a city with a relatively high level of Arsenic contamination.</span><span style="font-size:8.0pt;line-height:115%;
-font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"><o:p></o:p></span></p>
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-	<span id="vbid-5515b55f-jizfjywl"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >Lab Safety Biosafety Level 1 (BSL-1)Safety Guidelines If you work in a lab that is designated a BSL-1, the microbes there are not known to consistently cause disease in healthy adults and present minimal potential hazard to laboratory staff and the environment. An example of a microbe that is typically worked with at a BSL-1 is a nonpathogenic strain of E. coli</span>
+<div class="preview-title-holder removable-parent order-handle">
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+</div>
+<br class="lower-line-break" />
+<br class="upper-line-break" />
+<div class="preview-body-holder removable-parent order-handle">
+	<div id="vbid-c0c60978-rouzmtxs" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+		<p><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">UV light exposure in our experiments comes from 3 different places:</span></span></p><ul><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The gel imager that we used to take pictures of our DNA gels and paper tickets.</span></span></li><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The handheld UV lamp we used to preview the results of our tickets before imaging.</span></span></li><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The UV LEDs that we used in our imaging chamber with the VK3 camera.</span></span></li></ul><p><span style="font-size:12px;">To combat these issues:</span></p><ul><li style="text-align: left;"><span style="font-size:12px;"><span style="text-align: center;">T</span><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">he gel imager was enclosed in a box with internal imaging capabilities to avoid exposure to UV light. We also had an orange filter that was used to protect our eyes if we wanted to look at the gel directly.</span></span></li><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The handheld UV lamp was not enclosed in a box, so we wore a protective face mask when using it. We also made sure to point the lamp away from ourselves and our skin.</span></span></li><li><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">The UV LEDs in the VK3 camera were assembled inside a box to limit the exposure it had on our skin. This means turning off the LEDs when we were working on the assembly and making sure the box was closed when we tested the imaging design.</span></span></li></ul><p><span style="font-size:12px;"><span style="color: rgb(42, 42, 42); letter-spacing: 1.95px; text-align: center;">We understand the dangers of arsenic, so we always wore gloves when weighing out the chemicals and handling the arsenic solution. Any waste product or used tickets were placed in a container and disposed of through the environmental health chemical waste disposal program. </span></span></p>
+	</div>
 </div>
+<br class="lower-line-break" />
@@ Line 358: / Line 733: @@
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+		<p class="MsoNormal" style="text-align: center;"><span style="font-family: montserrat; font-size: 16px;">• Decontaminate all work surfaces with appropriate disinfectant after completion of work and after any spill or splash of potentially infectious or viable recombinant material. Suitable disinfectants include: 10% bleach solution, 70% ethanol solution or 2% wescodyne solution.</span><span style="font-size:8.0pt;line-height:115%;font-family:
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@@ Line 1,321: / Line 1,696: @@
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-		<p class="MsoNormal" style="text-align: center;"><span style="font-family:montserrat;"><span style="font-size:16px;"><span style="line-height: 115%;"></span></span></span><span style="font-family: montserrat; font-size: 16px;">• Decontaminate all cultures, stocks, and other potentially infectious or recombinant materials before disposal using an effective method.</span><span style="font-family:montserrat;"><span style="font-size:16px;"><span style="line-height: 115%;"></span></span></span><span style="font-size:8.0pt;line-height:115%;font-family:
+		<p class="MsoNormal" style="text-align: center;"><span style="font-family: montserrat; font-size: 16px;">• Using an effective method, decontaminate all cultures, stocks, and other potentially infectious or recombinant materials before disposal.</span><span style="font-size:8.0pt;line-height:115%;font-family:
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+<br class="upper-line-break" />
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+		<p class="MsoNormal" style="text-align: center;"><span style="line-height:1em;"><span style="font-size: 16px;">• </span><span id="docs-internal-guid-1b7da29c-f967-56fe-35c7-85a31760a18d"><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Anyone working in the lab needs to wear closed toe shoes, clothing covering down to at least the knee, and proper protective equipment as needed. </span></span></span><span style="font-size:8.0pt;line-height:115%;font-family:
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+	<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lightbox?action=raw&ctype=text/javascript"></script>
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