Difference between revisions of "Team:BostonU/Parts"

 
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<h1>Parts</h1>
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<p> HEloo Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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  <p class="wide-heading-type mainwrap align-center">OUR PARTS</p>
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<p class="inline-heading-type mainwrap">We submitted two composite parts to the registry: BBa_K2411005 and BBa_K2411008.</p>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411005" style="text-indent:0pt;">BBa_K2411005</a>: Forward Engineered Toehold 1 with deGFP gene</p>
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<p class="body-type mainwrap">This device encodes for a toehold that when activated expresses deGFP. It has an OR2-OR1 promoter, followed by the first forward engineered toehold sequence designed by Green et al. The deGFP follows the toehold, and the device ends with a T7 terminator. The part has been designed for and tested in an E. coli derived transcriptional translation cell free system. In order for the part to function, it needs to be used in conjunction with a trigger RNA. Characterization data can be view on our results page or on the registry.</p>
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<img src="https://static.igem.org/mediawiki/2017/1/1b/T--BostonU--Part_th2comp.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411008" style="text-indent:0pt;">BBa_K2411008</a>: Forward Engineered Toehold 2 with deGFP gene</p>
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<p class="body-type mainwrap">This device encodes for a toehold that when activated expresses deGFP. It has an OR2-OR1 promoter, followed by the second best forward engineered toehold sequence designed by Green et al. The deGFP follows the toehold, and the device ends with a T7 terminator. The part has been designed for and tested in an E. coli derived transcriptional translation cell free system. In order for the part to function, it needs to be used in conjunction with a trigger RNA. Characterization data can be view on our results page or on the registry.&nbsp;</p>
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<p class="body-type mainwrap">In addition to these composite parts, we created registry pages for the OR2-OR1 promoter, the T500 terminator, and both toehold architectures that we used in our composite parts.</p>
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<img src="https://static.igem.org/mediawiki/2017/0/08/T--BostonU--Part_th1comp.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411000" style="text-indent:0pt;">BBa_K2411000</a>: OR2-OR1 Promoter</p>
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<p class="body-type mainwrap">This is a constitutively active promoter that can be used in E. coli and E. coli derived transcription translation systems. This part is derived from the lambda bacteriaphage.</p>
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<img src="https://static.igem.org/mediawiki/2017/1/1f/T--BostonU--Part_or2or1.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411002" style="text-indent:0pt;">BBa_K2411002</a>: T500 Terminator</p>
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<p class="body-type mainwrap">This is a bacterial terminator.</p>
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<img src="https://static.igem.org/mediawiki/2017/4/42/T--BostonU--Part_t500.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411004" style="text-indent:0pt;">BBa_K2411004</a>: Toehold 1</p>
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<p class="body-type mainwrap">This sequence is the forward engineered toehold from Green et. al. This part contains the hairpin loop sequence that with the RBS. There is no gene associated with this toehold.</p>
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<img src="https://static.igem.org/mediawiki/2017/c/c8/T--BostonU--Part_th2.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap"><a href = "http://parts.igem.org/Part:BBa_K2411007" style="text-indent:0pt;">BBa_K2411007</a>: Toehold 2</p>
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<p class="body-type mainwrap">This sequence is the second best performing forward engineered toehold from Green et. al. There is no gene associated with this toehold.</p>
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<img src="https://static.igem.org/mediawiki/2017/7/74/T--BostonU--Part_th1.png" width = 25%></img>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap">In addition, the deGFP part utilized in our composite part was submitted under BBa_K2205001 by iGEM17_Newcastle.</p>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap">The sequences for the promoter, terminator, and deGFP were obtained from the pBEST plasmid used to Vincent Noireaux and his lab with their cell-free system [1].</p>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap">The sequences for the toeholds were obtained from Green et al. [2].</p>
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<p class="body-type mainwrap">A toehold switch part including a ribosomal binding site and a start codon.</p>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap">[1]Shin, Jonghyeon, and Vincent Noireaux. "Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70." Journal of biological engineering 4.1 (2010): 8.</p>
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<p class="body-type mainwrap">&nbsp;</p>
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<p class="body-type mainwrap">[2] Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.</p>
 
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM17 BostonU</groupparts>
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Latest revision as of 06:16, 31 October 2017

OUR PARTS