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| | + | <!--content begins here COMMENT LANDMARK--> |
| | + | <div id="This-is-where-it-begins"> |
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| | + | <div id="first-part-of-texts"></div> |
| | <h1>Hardware</h1> | | <h1>Hardware</h1> |
| − | <div class="column half_size"> | + | <h2>Introduction</h2> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><!--[if gte vml 1]><v:shapetype
| + | <p>To lower the barrier of synthetic biology to the public, we aimed to develop an integrated hardware platform that allows building a complex bio-product free of lab work and with minimal requirement of experience in synthetic biology for the designers. This platform comprises of an interface to a cloud server, a special tube to culture bacteria, and a liquid-handling robot to transfer liquid. Upon receiving the command, the gene circuits to produce a bio-product through a cloud server, bacteria representing the blocks in gene circuits will be inoculated into our special tube. The tube can separate signal molecule-containing supernatant from bacteria cell mass. The supernatant is transferred as desired to another tube through our robot, thus connecting two blocks in the circuits. With delicate gene circuits, a bio-product is produced. Therefore, the designers produce a bio-product without visiting bio-labs. We have built a low cost prototype hardware platform and showed it functioned properly.</p> |
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| + | <p>Here is a brief introduction of our hardware design. With our proper design and manufacture, we can make our prototype hardware within 1 day and with less than $180, which is very cost effective.</p> |
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| + | <p style="text-align: center;"><img src="#####" alt="Video Space Holder" width="1920" height="1080"></p> |
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| + | <h2>A. Interface with a cloud server.</h2> |
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| + | <p>As shown in the demo video, we have successfully built the prototype hardware platform and verified that this platform could be controlled remotely through our <a href="https://2017.igem.org/Team:Shanghaitech/Software">software interface</a>. It will work autonomously after receiving proper commands via internets or a cloud server.</p> |
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| + | <h2>B. Special tube to culture bacteria.</h2> |
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| + | <li><h2>Design</h2></li> |
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| + | <p>Our magic blocks are bacteria cultures with desired information processing ability. To connect bacteria with clear logic rules, bacteria need them to communication with each other according to gene circuits designed by the designers. We used quorum sensing system to communicate one bacteria population with another. The secreted signal chemicals need to be transferred between bacteria to induce further signal production. However, the transfer of bacteria cells should be avoided so as to reduce the interference between different bacteria populations. Therefore, we need to design a special culture tube (Fig.B) so that the culture supernatant can be easily sucked away and cells will stay in the original tubes. As shown below, to make our special tube, we glued two tubes together separated by a 0.22um membrane so that the bacteria will stay in the larger tube outside and signal chemicals can diffuse into the smaller tube inside. The supernatant inside of the smaller tube can be transferred out by our liquid handing robot as needed.</p> |
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| + | <p>< img src=" #####" alt=" special bacteria culture tube" width=" 1249" height=" 1408"/></p> |
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| + | <p>Fig.B The design of the special bacteria culture tube. Only supernatant that contains signal molecules can be transferred out to initiate further responses.</p> |
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| + | |
| − | font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Hardware</span></b><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:"Arial Unicode MS""><o:p></o:p></span></b></p>
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| − | | + | |
| − | <p class="MsoNormal"><b><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Introduction:<o:p></o:p></span></b></p>
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| − | <p class="MsoNormal" style="text-indent:21.3pt;mso-char-indent-count:2.24"><span lang="EN-US" style="font-size: 9.5pt; font-family: Arial, sans-serif;">To lower the barrier of | + | |
| − | synthetic biology to the public, we aimed to develop an integrated hardware | + | |
| − | platform that allows building a complex bio-product free of lab work and with | + | |
| − | minimal requirement of experience in synthetic biology for the designers. This | + | |
| − | platform comprises of an interface to a cloud server, a special tube to culture | + | |
| − | bacteria, and a liquid-handling robot to transfer liquid. Upon receiving the | + | |
| − | command, the gene circuits to produce a bio-product through a cloud server, | + | |
| − | bacteria representing the blocks in gene circuits will be inoculated into our | + | |
| − | special tube. The tube can separate signal molecule-containing supernatant from | + | |
| − | bacteria cell mass. The supernatant is transferred as desired to another tube | + | |
| − | through our robot, thus connecting two blocks in the circuits. With delicate | + | |
| − | gene circuits, a bio-product is produced. Therefore, the designers produce a | + | |
| − | bio-product without visiting bio-labs. We have built a low cost prototype | + | |
| − | hardware platform and showed it functioned properly.<o:p></o:p></span></p> | + | |
| − | | + | |
| − | <p class="MsoNormal" style="text-indent:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Here | + | |
| − | is a brief introduction of our hardware design. With our proper design and manufacture, | + | |
| − | we can make our prototype hardware within 1 day and with less than $180, which | + | |
| − | is very cost effective. <o:p></o:p></span></p> | + | |
| − | | + | |
| − | <p class="MsoNormal" style="text-indent:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Video:<o:p></o:p></span></p> | + | |
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| − | mso-font-kerning:0pt"><o:p></o:p></span></p>
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| − | <p class="MsoNormal" style="margin-left:18.0pt;text-indent:-18.0pt;mso-list:l2 level1 lfo1"><!--[if !supportLists]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif; | + | |
| − | mso-fareast-font-family:Arial">A.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";"> </span></span></b><!--[endif]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:"Arial Unicode MS"">Interface with a cloud server.<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p class="MsoNormal" style="margin-left:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">As | + | |
| − | shown in the demo video, we have successfully built the prototype hardware | + | |
| − | platform and verified that this platform could be controlled remotely through our | + | |
| − | software interface (</span><span lang="EN-US"><a href="https://2017.igem.org/Team:Shanghaitech/Software"> <span style="font-family: | + | |
| − | "Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">https://2017.igem.org/Team:Shanghaitech/Software</span></a></span><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">).
| + | |
| − | It will work autonomously after receiving proper commands via internets or a | + | |
| − | cloud server.<o:p></o:p></span></p> | + | |
| − | | + | |
| − | <p class="MsoNormal" style="margin-left:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS""> </span></p> | + | |
| − | | + | |
| − | <p class="MsoNormal" style="margin-left:18.0pt;text-indent:-18.0pt;mso-list:l2 level1 lfo1"><!--[if !supportLists]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:Arial">B.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";"> </span></span></b><!--[endif]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:"Arial Unicode MS"">Special tube to culture bacteria.<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:21.0pt;text-indent:-21.0pt; | + | |
| − | mso-char-indent-count:0;mso-list:l1 level1 lfo2"><!--[if !supportLists]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:Arial">1.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";"> </span></span></b><!--[endif]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:"Arial Unicode MS"">Design:<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p class="MsoNormal" style="text-indent:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Our | + | |
| − | magic blocks are bacteria cultures with desired information processing ability. | + | |
| − | To connect bacteria with clear logic rules, bacteria need them to communication | + | |
| − | with each other according to gene circuits designed by the designers. We used | + | |
| − | quorum sensing system to communicate one bacteria population with another. The | + | |
| − | secreted signal chemicals need to be transferred between bacteria to induce | + | |
| − | further signal production. However, the transfer of bacteria cells should be | + | |
| − | avoided so as to reduce the interference between different bacteria | + | |
| − | populations. Therefore, we need to design a special culture tube<span style="color:red"> </span>(Fig.B) so that the culture supernatant can be easily | + | |
| − | sucked away and cells will stay in the original tubes. As shown below, to make | + | |
| − | our special tube, we glued two tubes together separated by a 0.22um membrane so | + | |
| − | that the bacteria will stay in the larger tube outside and signal chemicals can | + | |
| − | diffuse into the smaller tube inside. The supernatant inside of the smaller | + | |
| − | tube can be transferred out by our liquid handing robot as needed. <o:p></o:p></span></p> | + | |
| − | | + | |
| − | <p class="MsoNormal" style="text-indent:18.0pt"><!--[if gte vml 1]><v:shape id="_x0000_s1026" | + | |
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| − | </tr> | + | |
| − | <tr>
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| − | <td></td>
| + | |
| − | <td><img width="250" height="282" src="https://static.igem.org/mediawiki/2017/8/88/T--Shanghaitech--hyp3hardware.jpg" v:shapes="_x0000_s1026"></td>
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| − | <!--[endif]--><span lang="EN-US" style="font-family:"Arial",sans-serif;
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| − | mso-fareast-font-family:"Arial Unicode MS""> </span><p></p>
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| − | <p class="MsoNormal" style="text-indent:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Fig.B
| + | |
| − | The design of the special bacteria culture tube. Only supernatant that contains | + | |
| − | signal molecules can be transferred out to initiate further responses. <o:p></o:p></span></p> | + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:21.0pt;text-indent:-21.0pt;
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| − | mso-char-indent-count:0;mso-list:l1 level1 lfo2"><!--[if !supportLists]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
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| − | mso-fareast-font-family:Arial">2.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";"> </span></span></b><!--[endif]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
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| − | mso-fareast-font-family:"Arial Unicode MS"">Manufacture<o:p></o:p></span></b></p>
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| − | | + | |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-family:"Arial",sans-serif;
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| − | mso-fareast-font-family:"Arial Unicode MS""> Specific steps:<o:p></o:p></span></p>
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| − | <p class="MsoListParagraph" style="margin-left:189.0pt;mso-char-indent-count:
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| − | 0"><!--[if gte vml 1]><v:shape id="图片_x0020_2" o:spid="_x0000_s1059" type="#_x0000_t75"
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| − | absolute;z-index:251663872;left:0px;margin-left:0px;margin-top:364px;
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| − | width:269px;height:151px"><img width="215" height="121" src="https://static.igem.org/mediawiki/2017/6/67/T--Shanghaitech--hyphardware3.jpg" alt="DSC09229" v:shapes="图片_x0020_2"></span><!--[endif]--><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Step1:
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| − | Cut a 15 ml centrifuge tube at the position of 0.6 ml. 5s pre-heating to soft
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| − | the tube is recommended.<o:p></o:p></span></p>
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| − | <p class="MsoListParagraph" style="margin-left:210.0pt;mso-char-indent-count:
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| − | "Arial Unicode MS""> </span></p>
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| − | <!--[if !mso]--></td> | + | <li><h2>Manufacture</h2></li> |
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| + | <p>Specific steps:</p> |
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| + | < div class=" image- container"><img src="#####" alt=" Cut position of a 15 ml tube" width=" 493" height=" 277" /></ div> |
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| + | <p>Step1: Cut a 15 ml centrifuge tube at the position of 0.6 ml. 5s pre-heating to soft the tube is recommended.</p> |
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| − | <p class="MsoNormal" align="left"><span lang="EN-US" style="color:yellow">Sealed tube with 0.22um membrane<o:p></o:p></span></p>
| + | |
| | </div> | | </div> |
| − | <!--[if !mso]--></td> | + | < div class=" image- text- container image- text- centered"><p>< img src="# ####" alt=" Arrow Down" height=" 200" width=" 100" /></ p>< /div> |
| − | </tr>
| + | <div class="image-text-container image-left"> |
| − | </tbody></table>
| + | <div class="image-container"><img src="#####" alt="Sealed tube with 0.22um membrane" width="670" height="377" /></div> |
| − | </span><!--[endif]--><!--[if !mso & !vml]--> <!--[endif]--><!--[if !vml]--></td>
| + | <p>Step2: Apply a layer of glue evenly to the exposed cross section of cut and then glue a piece of 0.22 um membrane to it. Apply additional layer of glue to strengthen the attachment between the membrane and the cross section.</p> |
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| + | |
| − | "Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Step3: In the
| + | |
| − | lid center of a 50 ml centrifuge tube, make a hole so that the 15 ml tube can
| + | |
| − | pass by. And then fix the 15 ml tube inside of the 50 ml tube with glue as
| + | |
| − | showing in the left. Now the bacteria can be culture in the 50 ml tube and the
| + | |
| − | signal containing supernatant can be removed from the 15 ml tube.<o:p></o:p></span></p>
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| − | mso-fareast-font-family:"Arial Unicode MS"">Liquid-handling robot<o:p></o:p></span></b></p>
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| − | mso-fareast-font-family:"Arial Unicode MS"">Design<o:p></o:p></span></b></p>
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| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
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| − | "Arial Unicode MS"">Considering the cost and manufacture difficulties, we decided
| + | |
| − | to DIY a liquid handling robot by reconfiguration of a 3D printer together with
| + | |
| − | a syringe pump. The ready-made interface port in the printer allows automatic
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| − | and remote control. We therefore can program the 3D printer rig to move the
| + | |
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| − | above. <o:p></o:p></span></p>
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| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
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| − | 10.5pt;font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Get
| + | |
| − | an X-Y-Z <span style="color:#2E3033;background:#F9FBFC">3 axes programmable platform</span></span><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">
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| + | |
| − | bought an anycubic-i3 3D printer for reconfiguration from Taobao: </span><span lang="EN-US"><a href="https://item.taobao.com/item.htm?id=530445909316"><span style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">https://item.taobao.com/item.htm?id=530445909316</span></a></span><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS""><o:p></o:p></span></p>
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| − | <p class=MsoNormal><span lang=EN-US style='color:yellow'>Attaching syringe
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| − | with baud rate 250000 to control both. Now the assembled platform with the 3D
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