This has been the first time we participate in igem competition and engage in the interlab study. The purpose of this years study is to figure out the fine difference between the plate readers all around the world. We are excited about the experiment and these are some datas we collected from interlab.
OD600 referencing point
Fluorensein Standard Curve
Florence Measurement
Collaboration
We get in touch with team Light Harvester in June through our instructor. Even though we’ve go though the HighSchool student handbook, we’re still lack of some biotechnology lab skill. And since our lab is specifically established for the igem project in this year, we are lack of equipment resources. As a result, we learned extraction and transformation of plasmids, standardized aseptic manipulation, the extraction and purification of DNA, and how to operate various instruments and so on. With all the knowledges we needed for completing the interlab, our team start the interlab in August 8th. We finished our final measurement of interlab with their assistants about plate reader and their help upon the cultivation of final. In the first week, we dilute the fluorescein and measure the fluorescein solution’s standard curve. In the week after that, we measured OD600 Reference point and fluorescein fluorescence standard curve using protocols. Other than communicate about the experimental design between two groups, our team help them to check english grammar use.
Our team is composed of a group of young and motivated students with helpful advisors from Shanghai United International School Wanyuan Campus. We all love synthetic biology. We aim to make a contribution for the society and improve the food safety issue.
