Difference between revisions of "Team:TUDelft/Main-Parts"
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| Line 153: | Line 153: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| + | <th>Fave Part</th> | ||
<th>Name</th> | <th>Name</th> | ||
<th>Type</th> | <th>Type</th> | ||
| Line 161: | Line 162: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> | ||
| + | |||
<td>Basic</td> | <td>Basic</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 68234</td> | <td>Secretory-abundant heat soluble protein (SAHS) 68234</td> | ||
| Line 168: | Line 171: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>♥</td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> | ||
| + | |||
<td>Basic</td> | <td>Basic</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 33020</td> | <td>Secretory-abundant heat soluble protein (SAHS) 33020</td> | ||
| Line 175: | Line 180: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| Line 182: | Line 188: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| Line 189: | Line 196: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306000 target="_blank">BBa_K2300600</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306000 target="_blank">BBa_K2300600</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| Line 196: | Line 204: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306012 target="_blank">BBa_K2306012</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306012 target="_blank">BBa_K2306012</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| Line 203: | Line 212: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306014 target="_blank">BBa_K2306014</a></td> | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306014 target="_blank">BBa_K2306014</a></td> | ||
| Line 211: | Line 221: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| Line 222: | Line 233: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| + | <th>Fave Part</th> | ||
<th>Name</th> | <th>Name</th> | ||
<th>Type</th> | <th>Type</th> | ||
| Line 230: | Line 242: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 237: | Line 250: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>♥</td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> | ||
| + | |||
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator</td> | <td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 244: | Line 259: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 251: | Line 267: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 258: | Line 275: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306001 target="_blank">BBa_K2306001</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306001 target="_blank">BBa_K2306001</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 265: | Line 283: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 277: | Line 296: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| + | <th>Fave Part</th> | ||
<th>Name</th> | <th>Name</th> | ||
<th>Type</th> | <th>Type</th> | ||
| Line 285: | Line 305: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 292: | Line 313: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>♥</td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> | ||
| + | <td>♥</td> | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator</td> | <td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator</td> | ||
| Line 299: | Line 322: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 306: | Line 330: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 313: | Line 338: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| Line 321: | Line 347: | ||
<tr> | <tr> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> | ||
| + | <td>♥</td> | ||
<td>Basic</td> | <td>Basic</td> | ||
<td>Secretory-abundant heat soluble protein (SAHS) 33020</td> | <td>Secretory-abundant heat soluble protein (SAHS) 33020</td> | ||
| Line 327: | Line 354: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| Line 334: | Line 362: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td></td> | ||
<td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
Revision as of 09:44, 31 October 2017
Parts
When constructing our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts we synthesised contain the coding sequence, RBS, promoter and terminators, if applicable. Therefore, these parts are composite. The different basic parts comprising the composite parts are documented separately, and also exist as separate biobricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. These basic parts were generated by using PCR and restriction ligation on the synthesised composite parts. We created a part collection comprised of 4 composite parts encoding for different Tardigrade Proteins (TDPs) and the 4 basic parts of these genes, all with elaborate characterisation so the user may choose the best protein for their project accordingly. For the composite parts of the TDPs we designed a new, strong RBS. This part does not exist separately. Additionally, we include the documentation of a basic part encoding the gene for Cas13a, derived from the plasmid pC011 from Gootenberg et al (2017). This biobrick does not exist separately. Finally, we submitted a composite part containing a nonsense spacer with flanking double repeats (with constitutive promoter), appropriate for use for Cas13a. This part is designed so that the user can easily change the spacer according to the sequence they wish to target. Find more info on this cool part on its page in the registry! We also documented this as a basic part without a promoter. This part does not exist separately.
In our project, we added to the characterisation of an existing Biobrick, the TolR, Periplasmic domain design by Gavin Sutton from iGEM Australia 2016. Additionally, our TolR-GFP composite part was based on the biobrick created by NTU Trontheim with some alterations, such as the removal of the RFP sequence. This enabled us to build on their knowledge and improve the function.
♥
Improved BioBrick / Further Characterisation
All Parts
Fave Part
Name
Type
Description
Designer
Length
BBa_K2306000
Coding
TorA-GFP fusion
Gabriella Tany
846
BBa_K2306001
Composite
TorA-GFP fusion (LacI promotor)
Gabriella Tany
1212
BBa_K2306002
Coding
Secretory-abundant heat soluble protein 68234 (SAHS 68234)
Guillermo Serena Ruiz
525
BBa_K2306003
Coding
Secretory-abundant heat soluble protein 33020 (SAHS 33020)
Guillermo Serena Ruiz
509
BBa_K2306005
Coding
Cytosolic-abundant heat soluble protein 106094 (CAHS 106094)
Guillermo Serena Ruiz
684
BBa_K2306006
Coding
Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)
Guillermo Serena Ruiz
681
BBa_K2306007
Composite
Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
768
♥
BBa_K2306008
Composite
Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
752
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
927
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
924
BBa_K2306012
Coding
Cas13a
Jeroen Jacques
3920
BBa_K2306013
Composite
Cas13a spacer with flanking double repeats, constitutive promoter
Jeroen Jacques and Jasper Veerman
134
BBa_K2306014
RBS
RBS from bacteriophage T7 gene 10 and Shine Dalgarno
Guillermo Serena Ruiz
30
BBa_K2306015
Other
Cas13a spacer with flanking double repeats
Jeroen Jacques and Jasper Veerman
99
Basic Parts
Fave Part
Name
Type
Description
Designer
Size (bp)
BBa_K2306002
Basic
Secretory-abundant heat soluble protein (SAHS) 68234
Guillermo Serena Ruiz
525
♥
BBa_K2306003
Basic
Secretory-abundant heat soluble protein (SAHS) 33020
Guillermo Serena Ruiz
509
BBa_K2306005
Basic
Cytosolic-abundant heat soluble protein (CAHS) 106094
Guillermo Serena Ruiz
684
BBa_K2306006
Basic
Cytosolic-abundant heat soluble protein (CAHS) 94205
Guillermo Serena Ruiz
681
BBa_K2300600
Basic
TolR GFP
Gabriella Tany
681
BBa_K2306012
Basic
Cas13a
Jeroen Jacques
3456
BBa_K2306014
RBS
RBS from bacteriophage T7 gene 10 and Shine Dalgarno
Guillermo Serena Ruiz
30
BBa_K2306015
Basic
Spacer with flanking double repeats for Cas13a
Jeroen Jacques, Jasper Veerman
99
Composite Parts
Fave Part
Name
Type
Description
Designer
Size (bp)
BBa_K2306007
Composite
Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
833
♥
BBa_K2306008
Composite
Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
817
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
992
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
989
BBa_K2306001
Composite
TolR GFP fusion with inducible LacI promotor, RBS and double terminator
Gabriella Tany
681
BBa_K2306015
Composite
Cas13a spacer with flanking double repeats, constitutive promoter
Jeroen Jacques, Jasper Veerman
134
Part Collection
Fave Part
Name
Type
Description
Designer
Size (bp)
BBa_K2306007
Composite
Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
833
♥
BBa_K2306008
♥
Composite
Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
817
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
992
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promotor, RBS and double terminator
Guillermo Serena Ruiz
989
BBa_K2306002
Basic
Secretory-abundant heat soluble protein (SAHS) 68234
Guillermo Serena Ruiz
525
BBa_K2306003
♥
Basic
Secretory-abundant heat soluble protein (SAHS) 33020
Guillermo Serena Ruiz
509
BBa_K2306005
Basic
Cytosolic-abundant heat soluble protein (CAHS) 106094
Guillermo Serena Ruiz
684
BBa_K2306006
Basic
Cytosolic-abundant heat soluble protein (CAHS) 94205
Guillermo Serena Ruiz
681
