Difference between revisions of "Team:Jilin China/Improve"

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<div class="banner"><div class="menu">Improvement</div><img src="https://static.igem.org/mediawiki/2017/b/b1/T--Jilin_China--_sec_bg_t.jpg"></div>
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<div class="column full_size">
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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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    <div class="h1_title">1.Improvement in dector method in DmpR’s response intensity.</div>
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<div class="h1_content">
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<h3>Gold Medal Criterion #2</h3>
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<p>(1) We realize the quantitative measurement in DmpR’s response intensity using Reallia Luciferase. Compared to GFP <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/DmpR">team Peking-2013</a> use, we can detect DmpR’s inducing strength more accurately.
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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<div class="pic_box center">
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    <img src="https://static.igem.org/mediawiki/2017/4/4f/T--Jilin_China--improvement01.png"  /><br />
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<br><br>
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    <img src="https://static.igem.org/mediawiki/2017/1/1d/T--Jilin_China--improvement02.png"  /><br />
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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    Figure 1. DmpR’s circuit. (A) Peking-2013 (B) Jilin-2017
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    </div>
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</p>
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<p>(2) Renilla Luciferase enable us conduct the measurement in vivo, which provide a easier and faster method in detecting.</p>
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</div>
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<div class="h1_title">2.Improvement in response intensity toward certain phenolics.</div>
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<div class="h1_content">
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<p>Team Jilin China in 2017 do several mutagenesis in sensor domain of DmpR. To compare the difference between <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/DmpR">Peking-2013 DmpR</a> and our mutated DmpR downstream of the same constitutive promoter, we chose phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol as the inducer. Expression level of Renilla Luciferase downstream of dmp operon (P0 promoter) was used to indicate the response intensity towards different phenolics. Comparisons are based on ratio of different DmpR’s response and mock’s toward phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol respectively.
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<div class="pic_box center">
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    <img src="https://static.igem.org/mediawiki/2017/b/be/T--Jilin_China--improvement03.png"  /><br />
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    Figure 1. The device we constructed to do comparison between peking-2013 dmpR and our mutated DmpR
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    </div>
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</p><br />
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<p>
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It turned out that both DmpR can response to phenol, 2-CP, pyrocatechol. However, compared to Peking-DmpR, our DmR showed better response to phenol and pyrocatechol.
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<div class="pic_box center">
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    <img src="https://static.igem.org/mediawiki/2017/7/7c/T--Jilin_China--improvement04.png"  /><br />
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    Figure 2. DmpR’s response sensitivity toward different phenolics. Jilin-2017-DmpR showed better response to phenol and pyrocatechol.
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{{Jilin_footer}}

Revision as of 10:46, 31 October 2017

1.Improvement in dector method in DmpR’s response intensity.

(1) We realize the quantitative measurement in DmpR’s response intensity using Reallia Luciferase. Compared to GFP team Peking-2013 use, we can detect DmpR’s inducing strength more accurately.



Figure 1. DmpR’s circuit. (A) Peking-2013 (B) Jilin-2017

(2) Renilla Luciferase enable us conduct the measurement in vivo, which provide a easier and faster method in detecting.

2.Improvement in response intensity toward certain phenolics.

Team Jilin China in 2017 do several mutagenesis in sensor domain of DmpR. To compare the difference between Peking-2013 DmpR and our mutated DmpR downstream of the same constitutive promoter, we chose phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol as the inducer. Expression level of Renilla Luciferase downstream of dmp operon (P0 promoter) was used to indicate the response intensity towards different phenolics. Comparisons are based on ratio of different DmpR’s response and mock’s toward phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol respectively.


Figure 1. The device we constructed to do comparison between peking-2013 dmpR and our mutated DmpR


It turned out that both DmpR can response to phenol, 2-CP, pyrocatechol. However, compared to Peking-DmpR, our DmR showed better response to phenol and pyrocatechol.


Figure 2. DmpR’s response sensitivity toward different phenolics. Jilin-2017-DmpR showed better response to phenol and pyrocatechol.