Cas9 & Cpf1 secretion
and activity
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<img width="100%" style="margin-top: -105px;" src="https://static.igem.org/mediawiki/2017/0/03/Uugiel.jpg"> | <img width="100%" style="margin-top: -105px;" src="https://static.igem.org/mediawiki/2017/0/03/Uugiel.jpg"> | ||
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<b>Background</b> Bio Inspired Innovation<br> | <b>Background</b> Bio Inspired Innovation<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| − | <span | + | <span class="team-duties"> |
› Experimental - Secreted Cas9 and Cpf1<br> | › Experimental - Secreted Cas9 and Cpf1<br> | ||
› Human Practices - Application | › Human Practices - Application | ||
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<div class="team-bio" style="height: 85px;"> | <div class="team-bio" style="height: 85px;"> | ||
Hey, I'm Giel (22), a second-year Bio Inspired Innovation master student and one of the two team captains of this iGEM team. | Hey, I'm Giel (22), a second-year Bio Inspired Innovation master student and one of the two team captains of this iGEM team. | ||
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I did my bachelor Molecular Life Sciences in Groningen, where I first heard about the iGEM competition. However when I arrived in Utrecht, I learned that they never participated before. So this makes me extra proud to be a member of this first ever iGEM team! | I did my bachelor Molecular Life Sciences in Groningen, where I first heard about the iGEM competition. However when I arrived in Utrecht, I learned that they never participated before. So this makes me extra proud to be a member of this first ever iGEM team! | ||
| − | < | + | <div class="lb"></div> |
What I like about iGEM is that it's completely our own project and that we tackle a real-world problem... So if we work hard enough, we might actually make the world a little better! | What I like about iGEM is that it's completely our own project and that we tackle a real-world problem... So if we work hard enough, we might actually make the world a little better! | ||
| − | < | + | <div class="lb"></div> |
When I'm not busy with iGEM, or while we're waiting for our experiments, I like to read, draw, paint or, when the weather is nice, to go for a run! | When I'm not busy with iGEM, or while we're waiting for our experiments, I like to read, draw, paint or, when the weather is nice, to go for a run! | ||
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<img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/4/43/Uuouafa.jpg"> | <img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/4/43/Uuouafa.jpg"> | ||
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<b>Background</b> Biofabrication<br> | <b>Background</b> Biofabrication<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
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| − | › Experimental - MESA replication/Secreted Cas9 and Cpf1<br> | + | › Experimental - MESA<br> replication/Secreted Cas9 and Cpf1<br> |
› Funding & Sponsoring<br> | › Funding & Sponsoring<br> | ||
› Biobricks | › Biobricks | ||
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Hello, my name is Ouafa (23) and I’m a second-year master student in Biofabrication. | Hello, my name is Ouafa (23) and I’m a second-year master student in Biofabrication. | ||
| − | + | <div class="lb"></div> | |
I have the honour of being one of the team captains of Utrecht’s first iGEM team ever. For me, iGEM keeps science fun since we can apply our own skills and ideas in a completely original concept. Every day together with our diverse team has been such an amazing experience. I am super proud of the personal growth of each individual team member and I’m looking forward to see where our project will bring us! | I have the honour of being one of the team captains of Utrecht’s first iGEM team ever. For me, iGEM keeps science fun since we can apply our own skills and ideas in a completely original concept. Every day together with our diverse team has been such an amazing experience. I am super proud of the personal growth of each individual team member and I’m looking forward to see where our project will bring us! | ||
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<img width="100%" style="margin-top: -90px;" src="https://static.igem.org/mediawiki/2017/c/c4/Uulishi.jpg"> | <img width="100%" style="margin-top: -90px;" src="https://static.igem.org/mediawiki/2017/c/c4/Uulishi.jpg"> | ||
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<b>Background</b> Pharmacy<br> | <b>Background</b> Pharmacy<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
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| − | + | › Human Practices - Application/Safety/Design<br> | |
| − | + | › Experimental - Assembly/InterLab Study<br> | |
| − | + | › Funding & Sponsoring | |
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Hi! I'm Lishi (21), a first year master student in Pharmacy at Utrecht University. After that, I want to become a hospital pharmacist. | Hi! I'm Lishi (21), a first year master student in Pharmacy at Utrecht University. After that, I want to become a hospital pharmacist. | ||
| + | <div class="lb"></div> | ||
I joined the Utrecht iGEM team because it is a unique opportunity to learn new things. One of these things is that I get to work in the lab and do those things that I’ve only learned the theoretical parts of. I’m also part of the human practices group along with Stefan, Pam and Dorien. Human practices is the part where we have to figure out how our work affects the world and how the world affects our work. For example, we have interviewed many people to figure out what they think of our project and how we can use their knowledge to improve it. This makes the project very diverse and with this amazing team, we had a great time! | I joined the Utrecht iGEM team because it is a unique opportunity to learn new things. One of these things is that I get to work in the lab and do those things that I’ve only learned the theoretical parts of. I’m also part of the human practices group along with Stefan, Pam and Dorien. Human practices is the part where we have to figure out how our work affects the world and how the world affects our work. For example, we have interviewed many people to figure out what they think of our project and how we can use their knowledge to improve it. This makes the project very diverse and with this amazing team, we had a great time! | ||
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<img width="100%" style="margin-top: -80px;" src="https://static.igem.org/mediawiki/2017/6/66/Uusam.jpg"> | <img width="100%" style="margin-top: -80px;" src="https://static.igem.org/mediawiki/2017/6/66/Uusam.jpg"> | ||
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<b>Background</b> Pharmaceutical Sciences<br> | <b>Background</b> Pharmaceutical Sciences<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
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| − | + | › Wiki - design & coding<br> | |
| − | + | › Experimental - Assembly<br> | |
| − | + | › Biobricks<br> | |
| − | + | › Public Relations | |
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<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
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| − | Hey, I am Sam (24) and currently in my final year of the Drug Innovation master's program. Quite a switch from my original path to become a pharmacist but I quickly found out that spending the rest of my days in pharmacy would drive me crazy. Instead, I went to pursue research. I love creating (profitable) things and science gives me an outlet to go wild with ideas. And let’s be honest, what’s more exciting than creating new therapies for diseases? | + | Hey, I am Sam (24) and currently in my final year of the Drug Innovation master's program. Quite a switch from my original path to become a pharmacist but I quickly found out that spending the rest of my days in a pharmacy would drive me crazy. |
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| + | Instead, I went to pursue research. I love creating (profitable) things and science gives me an outlet to go wild with ideas. And let’s be honest, what’s more exciting than creating new therapies for diseases? | ||
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Since science to me is synonymous to creating, I found participating in iGEM to be a no-brainer. The idea is that you start from scratch and try to have something tangible in the end. So far, it has been a really fun journey! | Since science to me is synonymous to creating, I found participating in iGEM to be a no-brainer. The idea is that you start from scratch and try to have something tangible in the end. So far, it has been a really fun journey! | ||
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<img width="100%" style="margin-top: -90px;" src="https://static.igem.org/mediawiki/2017/b/be/Uumerel.jpg"> | <img width="100%" style="margin-top: -90px;" src="https://static.igem.org/mediawiki/2017/b/be/Uumerel.jpg"> | ||
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<b>Background</b> Regenerative Medicine and Technology<br> | <b>Background</b> Regenerative Medicine and Technology<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
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› Experimental - Assembly<br> | › Experimental - Assembly<br> | ||
› Biobricks<br> | › Biobricks<br> | ||
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Hey, my name is Merel (22) and I am a master student Regenerative Medicine and Technology in Utrecht. | Hey, my name is Merel (22) and I am a master student Regenerative Medicine and Technology in Utrecht. | ||
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I joined the iGEM Utrecht team because I am very excited about working on a solution to a real problem, with an awesome diverse team. For our project, I have brought my enthusiasm and mad pipetting skills with me to the lab. In my free time, I like to go horse riding, play “korfball” (it’s a Dutch thing) or bake cakes. | I joined the iGEM Utrecht team because I am very excited about working on a solution to a real problem, with an awesome diverse team. For our project, I have brought my enthusiasm and mad pipetting skills with me to the lab. In my free time, I like to go horse riding, play “korfball” (it’s a Dutch thing) or bake cakes. | ||
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<img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/1/1e/Uupamela.jpg"> | <img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/1/1e/Uupamela.jpg"> | ||
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<b>Background</b> Biofabrication<br> | <b>Background</b> Biofabrication<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
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› Human Practices - Design/Safety/Application<br> | › Human Practices - Design/Safety/Application<br> | ||
› InterLab study | › InterLab study | ||
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<div class="team-bio" style="height: 61px;"> | <div class="team-bio" style="height: 61px;"> | ||
Hi everyone! My name is Pamela (22) and I am a master student Biofabrication in Utrecht! I am proud to call myself member of the first iGEM team Utrecht has ever had! To experience a project from the first mind-twisting brainstorm sessions to the final presentations in Boston is, in my eyes, a once in a life-time opportunity!: During the meetings, it is fun to see how the creativity and knowledge of such a diverse group results in really cool ideas! | Hi everyone! My name is Pamela (22) and I am a master student Biofabrication in Utrecht! I am proud to call myself member of the first iGEM team Utrecht has ever had! To experience a project from the first mind-twisting brainstorm sessions to the final presentations in Boston is, in my eyes, a once in a life-time opportunity!: During the meetings, it is fun to see how the creativity and knowledge of such a diverse group results in really cool ideas! | ||
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Facts you should know about me: In my free time I like to be sporty (yoga/running) and have drinks with friends! I love to travel and experience new cultures and for me the best holiday is an outdoor hike/canoeing/trekking trip! Cakes and desserts are the best food in this world, especially Tompouchen! | Facts you should know about me: In my free time I like to be sporty (yoga/running) and have drinks with friends! I love to travel and experience new cultures and for me the best holiday is an outdoor hike/canoeing/trekking trip! Cakes and desserts are the best food in this world, especially Tompouchen! | ||
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<img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/d/d8/Uuali.jpg"> | <img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/d/d8/Uuali.jpg"> | ||
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<b>Background</b> Pharmacy<br> | <b>Background</b> Pharmacy<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
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| − | + | › Experimental - Cas9 & Cpf1 secreti…<br> | |
| − | + | › Funding & Sponsoring | |
</span> | </span> | ||
<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
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Hi! My name is Ali (23) and I am a third year graduate student Pharmacy. I'm currently finishing my last internships and hope to kick off a great career being an industrial pharmacist next year. | Hi! My name is Ali (23) and I am a third year graduate student Pharmacy. I'm currently finishing my last internships and hope to kick off a great career being an industrial pharmacist next year. | ||
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I think freshman-year-me would never have guessed I’d be part of an interdisciplinary team of students competing in an international synthetic biology competition. But forget about him. For me, iGEM is a great way to learn new things. The aspects that really appeal to me are the novelty of the field, the integration of engineering and biology and the interdisciplinarity. It has been a pleasure being part of Utrecht's very first iGEM team and I am proud of the great things we have accomplished. | I think freshman-year-me would never have guessed I’d be part of an interdisciplinary team of students competing in an international synthetic biology competition. But forget about him. For me, iGEM is a great way to learn new things. The aspects that really appeal to me are the novelty of the field, the integration of engineering and biology and the interdisciplinarity. It has been a pleasure being part of Utrecht's very first iGEM team and I am proud of the great things we have accomplished. | ||
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<img width="100%" style="margin-top: -95px;" src="https://static.igem.org/mediawiki/2017/b/b4/Uudorien.jpg"> | <img width="100%" style="margin-top: -95px;" src="https://static.igem.org/mediawiki/2017/b/b4/Uudorien.jpg"> | ||
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<b>Background</b> Regenerative Medicine and Technology<br> | <b>Background</b> Regenerative Medicine and Technology<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
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| − | + | › Experimental - MESA replication<br> | |
| − | + | › Human Practices - Application/Safety/Design | |
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Hi, I am Dorien (21), a master student in Regenerative Medicine and Technology. This is an inspiring master which shows me all aspects of this interdisciplinary field. Aside from lab skills, I am taught how to communicate, present and cooperate with others. Skills that have improved during iGEM. | Hi, I am Dorien (21), a master student in Regenerative Medicine and Technology. This is an inspiring master which shows me all aspects of this interdisciplinary field. Aside from lab skills, I am taught how to communicate, present and cooperate with others. Skills that have improved during iGEM. | ||
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During my time at the university, I discovered that we need a more efficient way to use the knowledge that is discovered in the lab. Knowledge should be translated in order for it to be used in the wider world. This way, actual patients benefit from the knowledge obtained in the lab. I think it is important that what is researched is of use to society and that is why I joined iGEM. Here, we apply fundamental research by developing a DNA biosensor while considering how our biosensor affects society. We hope our sensor can be used as an easy tool to detect serious diseases in an early stage. It is awesome to join this multidisciplinary team of very passionate students and supervisors, all with creative minds, and I believe this will bring us closer to reaching our final goal. | During my time at the university, I discovered that we need a more efficient way to use the knowledge that is discovered in the lab. Knowledge should be translated in order for it to be used in the wider world. This way, actual patients benefit from the knowledge obtained in the lab. I think it is important that what is researched is of use to society and that is why I joined iGEM. Here, we apply fundamental research by developing a DNA biosensor while considering how our biosensor affects society. We hope our sensor can be used as an easy tool to detect serious diseases in an early stage. It is awesome to join this multidisciplinary team of very passionate students and supervisors, all with creative minds, and I believe this will bring us closer to reaching our final goal. | ||
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<b>Background</b> Molecular and Cellular Life Sciences<br> | <b>Background</b> Molecular and Cellular Life Sciences<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| − | <span | + | <span class="team-duties"> |
| − | + | › Experimental - Modeling<br> | |
| − | + | › Public Relations<br> | |
| − | + | › Design & Art | |
</span> | </span> | ||
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Hey, I am Leander (21), first-year Molecular and Cellular Life Sciences Master-student and one of the two non-dutchies in the team. My scientific journey began in Berlin where I studied everything from molecular biology to the mating sounds of tree frogs as part of my bachelors. After that, I decided to follow my passion for molecular biology and biochemistry by doing two internships in synthetic biology and enzyme design. I discovered that molecules are just my thing but at the same time realized that lab work is more tedious than my chemistry books suggested, which is why I currently spend my days studying protein-peptide interactions using nothing but a computer and highly concentrated coffee. | Hey, I am Leander (21), first-year Molecular and Cellular Life Sciences Master-student and one of the two non-dutchies in the team. My scientific journey began in Berlin where I studied everything from molecular biology to the mating sounds of tree frogs as part of my bachelors. After that, I decided to follow my passion for molecular biology and biochemistry by doing two internships in synthetic biology and enzyme design. I discovered that molecules are just my thing but at the same time realized that lab work is more tedious than my chemistry books suggested, which is why I currently spend my days studying protein-peptide interactions using nothing but a computer and highly concentrated coffee. | ||
| + | <div class="lb"></div> | ||
For me, iGEM is a unique opportunity to break the cycle of doing research and a simulation to test my own research skills in a fun environment. Finally, at the risk of sounding sappy, I am super happy to be part of this large and truly diverse team and I am amazed by the effort everybody puts into this. To be fair, the Dutch weather is lousy so there is no incentive whatsoever to go outside. | For me, iGEM is a unique opportunity to break the cycle of doing research and a simulation to test my own research skills in a fun environment. Finally, at the risk of sounding sappy, I am super happy to be part of this large and truly diverse team and I am amazed by the effort everybody puts into this. To be fair, the Dutch weather is lousy so there is no incentive whatsoever to go outside. | ||
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<img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/c/ca/Uuquincy.jpg"> | <img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/c/ca/Uuquincy.jpg"> | ||
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<b>Background</b> Molecular and Cellular Life Sciences<br> | <b>Background</b> Molecular and Cellular Life Sciences<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| − | <span | + | <span class="team-duties"> |
| − | + | › Experimental - Secreted Cpf1 and Cas9<br> | |
| − | + | › Design & Art<br> | |
| − | + | › Wiki - vector art | |
</span> | </span> | ||
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Hey! My name is Quincy (23) and I am currently doing the master programme “Molecular and Cellular Life Sciences” to further my career in the fields of cell biology, molecular biology, and microbiology. | Hey! My name is Quincy (23) and I am currently doing the master programme “Molecular and Cellular Life Sciences” to further my career in the fields of cell biology, molecular biology, and microbiology. | ||
I have an uncanny fascination for mushrooms and fungi and I spend much time with identifying these beautiful life-forms. So, if you are in Utrecht and you see a guy randomly crawling on the forest floor to hunt for mushrooms, then that’ll probably be me. Because of that, some tend to call me “funguy”. I love drawing and doing creative or artsy projects. | I have an uncanny fascination for mushrooms and fungi and I spend much time with identifying these beautiful life-forms. So, if you are in Utrecht and you see a guy randomly crawling on the forest floor to hunt for mushrooms, then that’ll probably be me. Because of that, some tend to call me “funguy”. I love drawing and doing creative or artsy projects. | ||
| + | <div class="lb"></div> | ||
Participating in the iGEM competition is a unique opportunity to participate in an interdisciplinary and international collaboration of motivated students. Although my primary focus is fundamental research, it’s really nice to also be involved in the implementation and human practices side of research. Also, I’m really proud to be part of the funniest and quirkiest iGEM team of 2017. Really, it’s true, it’s a fact. Period. | Participating in the iGEM competition is a unique opportunity to participate in an interdisciplinary and international collaboration of motivated students. Although my primary focus is fundamental research, it’s really nice to also be involved in the implementation and human practices side of research. Also, I’m really proud to be part of the funniest and quirkiest iGEM team of 2017. Really, it’s true, it’s a fact. Period. | ||
| − | < | + | <div class="lb"></div> |
PS: Bananas are actually berries | PS: Bananas are actually berries | ||
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<img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/8/88/Uustefan.jpg"> | <img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/8/88/Uustefan.jpg"> | ||
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<b>Background</b> Biofabrication<br> | <b>Background</b> Biofabrication<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| − | <span | + | <span class="team-duties"> |
| − | + | › Human Practices - Design/Safety/Application | |
</span> | </span> | ||
<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| − | <div class="team-bio"> | + | <div class="team-bio" style="height: 85px;"> |
Hey there. My name is Stefan (24) and I am in the second year of my Biofabrication MSc study – along with our team captain Ouafa. I am the second non-Dutchie of the Utrecht iGEM team. | Hey there. My name is Stefan (24) and I am in the second year of my Biofabrication MSc study – along with our team captain Ouafa. I am the second non-Dutchie of the Utrecht iGEM team. | ||
| − | + | <div class="lb"></div> | |
Working on iGEM has been quite a unique and wonderful experience. What I really enjoy about the project is that it pushes you to think about far more than just the science side of your project. For a great project it is important that the team has thought about the real societal and human impacts such a project would have. This means that it is important to have public outreach and dialogue with people that would be affected by your genetically engineered machine! This aspect of iGEM is what makes the iGEM project far bigger than just another research project, and for me, very fascinating. | Working on iGEM has been quite a unique and wonderful experience. What I really enjoy about the project is that it pushes you to think about far more than just the science side of your project. For a great project it is important that the team has thought about the real societal and human impacts such a project would have. This means that it is important to have public outreach and dialogue with people that would be affected by your genetically engineered machine! This aspect of iGEM is what makes the iGEM project far bigger than just another research project, and for me, very fascinating. | ||
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<b>Background</b> Biology<br> | <b>Background</b> Biology<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| − | <span | + | <span class="team-duties"> |
| − | + | › Experimental - Secreted cas9 and cpf1<br> | |
| − | + | › Public Relations | |
</span> | </span> | ||
<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| − | <div class="team-bio"> | + | <div class="team-bio" style="height: 85px;"> |
Hey, my name is Kewin (21) and I’m a third-year student of the Biology bachelor in Utrecht. To be fair, I find it pretty hard to find a direction to follow within the broadness of biology, but iGEM gave me a spark and a chance to not only meet the field of synthetic biology, but also to actually apply it. It’s really cool to start from the bottom up, to get together and discuss what we are going to do, how we will do it and then also actually achieve it, hopefully. | Hey, my name is Kewin (21) and I’m a third-year student of the Biology bachelor in Utrecht. To be fair, I find it pretty hard to find a direction to follow within the broadness of biology, but iGEM gave me a spark and a chance to not only meet the field of synthetic biology, but also to actually apply it. It’s really cool to start from the bottom up, to get together and discuss what we are going to do, how we will do it and then also actually achieve it, hopefully. | ||
| − | + | <div class="lb"></div> | |
Having an awesome group of mixed backgrounds shows me how you can tackle the same idea from different viewpoints and I think we complement each other very well. This experience is completely different from normal coursework, because it matters what and why you do the things you do. Not only for the successful outcome of the experiments, but you also need to consider societal implications, something I didn’t really learn in my bachelor. | Having an awesome group of mixed backgrounds shows me how you can tackle the same idea from different viewpoints and I think we complement each other very well. This experience is completely different from normal coursework, because it matters what and why you do the things you do. Not only for the successful outcome of the experiments, but you also need to consider societal implications, something I didn’t really learn in my bachelor. | ||
| − | + | <div class="lb"></div> | |
And the best part is: They sent us buttons and stickers! | And the best part is: They sent us buttons and stickers! | ||
| − | So yeah, hurray for iGEM, woohoo! | + | <div class="lb"></div> |
| + | So yeah, hurray for iGEM, woohoo! | ||
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<img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/c/cb/Uuteun.jpg"> | <img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/c/cb/Uuteun.jpg"> | ||
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<b>Background</b> Molecular and Cellular Life Sciences<br> | <b>Background</b> Molecular and Cellular Life Sciences<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| − | <span | + | <span class="team-duties"> |
| − | + | › Experimental - MESA replication/Assembly/Secreted Cpf1 and Cas9<br> | |
| − | + | › Public Relations<br> | |
| − | + | › Funding & Sponsoring<br> | |
| − | + | › Biobricks | |
</span> | </span> | ||
<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| − | <div class="team-bio"> | + | <div class="team-bio" style="height: 37px;"> |
Hey, I’m Theun (23). I am a first year master student Molecular and Cellular Life Sciences. I’ve always been interested in biology, but where my interest started as an obsession with animals, I am now more interested in cells and how I can make them do something useful and/or cool. That’s why I was instantly sold when I first heard about iGEM. It gave me the opportunity to start a project from scratch that, maybe, will actually be useful someday. As a bonus, it also allows me to satiate my inner Dr Frankenstein! | Hey, I’m Theun (23). I am a first year master student Molecular and Cellular Life Sciences. I’ve always been interested in biology, but where my interest started as an obsession with animals, I am now more interested in cells and how I can make them do something useful and/or cool. That’s why I was instantly sold when I first heard about iGEM. It gave me the opportunity to start a project from scratch that, maybe, will actually be useful someday. As a bonus, it also allows me to satiate my inner Dr Frankenstein! | ||
| − | + | <div class="lb"></div> | |
When I’m not looking up protocols and articles, I like to read, watch way too much Netflix, attempt to play guitar, practice jiu jitsu and I can make the best tiramisu you’ll ever taste. | When I’m not looking up protocols and articles, I like to read, watch way too much Netflix, attempt to play guitar, practice jiu jitsu and I can make the best tiramisu you’ll ever taste. | ||
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<img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/6/6d/Uurawan.JPG"> | <img width="100%" style="margin-top: -75px;" src="https://static.igem.org/mediawiki/2017/6/6d/Uurawan.JPG"> | ||
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<b>Background</b> Pharmacy<br> | <b>Background</b> Pharmacy<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| − | <span | + | <span class="team-duties"> |
| − | + | › Experimental - MESA replication/Secreted Cpf1 and Cas9<br> | |
| − | + | › Funding & Sponsoring<br> | |
| − | + | › Public Relations | |
</span> | </span> | ||
<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| − | <div class="team-bio"> | + | <div class="team-bio" style="height: 109px;"> |
I am Rawan, and currently in the final year of my master’s in pharmacy. The field of synthetic biology is partly new ground for me, but that does not make it any less fun. What struck me immediately about iGEM was the collaborative effort with people from multiple disciplines. Since this is very limited in my program, I thought it was an awesome opportunity to be part of the first iGEM team in Utrecht. | I am Rawan, and currently in the final year of my master’s in pharmacy. The field of synthetic biology is partly new ground for me, but that does not make it any less fun. What struck me immediately about iGEM was the collaborative effort with people from multiple disciplines. Since this is very limited in my program, I thought it was an awesome opportunity to be part of the first iGEM team in Utrecht. | ||
| + | <div class="lb"></div> | ||
Being able to work on both science as well as other activities, such as funding and human practices, completes the experience and is very useful in the future. Doing new things is very inspiring to me, and I feel like we are doing exactly that with iGEM. Having the opportunity to work on this project with a group of like-minded students and inspiring supervisors is an exciting experience. | Being able to work on both science as well as other activities, such as funding and human practices, completes the experience and is very useful in the future. Doing new things is very inspiring to me, and I feel like we are doing exactly that with iGEM. Having the opportunity to work on this project with a group of like-minded students and inspiring supervisors is an exciting experience. | ||
| + | <div class="lb"></div> | ||
Outside of the iGEM, I like to read, do sports, travel, listen to music and hang out with friends. Looking forward to see what the project brings! | Outside of the iGEM, I like to read, do sports, travel, listen to music and hang out with friends. Looking forward to see what the project brings! | ||
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<b>Background</b> Molecular and Cellular Life Sciences<br> | <b>Background</b> Molecular and Cellular Life Sciences<br> | ||
<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| − | <span | + | <span class="team-duties"> |
| − | + | › Human Practices - Design/Safety<br> | |
| − | + | › Wiki - content editor<br> | |
| − | + | › Experimental - Modeling<br> | |
| − | + | › Biobricks<br> | |
| − | + | › Lab management | |
</span> | </span> | ||
<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
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| − | Hi, I am Glenn. I'm a student of Utrecht's Molecular & Cellular Life Science master-programme, currently doing a project at the Theoretical Biology group. I work on temperate phage decision-making by constructing an individual based model. Yes, most of my time is spent staring at a computer screen, wondering why code doesn't work. Small surprise; the group has me working on the wiki. | + | Hi, I am Glenn (). I'm a student of Utrecht's Molecular & Cellular Life Science master-programme, currently doing a project at the Theoretical Biology group. I work on temperate phage decision-making by constructing an individual based model. Yes, most of my time is spent staring at a computer screen, wondering why code doesn't work. Small surprise; the group has me working on the wiki. |
| − | + | <div class="lb"></div> | |
I did my BSc in Groningen and, honestly, I occasionally miss working in a lab, a void easily filled by participating in the iGEM. In the team, I tend to be the cynic who takes notes. Outside of work, I often find myself writing. I like stories, particularly short SF and heroic fantasy, but I read far less than I'd like. | I did my BSc in Groningen and, honestly, I occasionally miss working in a lab, a void easily filled by participating in the iGEM. In the team, I tend to be the cynic who takes notes. Outside of work, I often find myself writing. I like stories, particularly short SF and heroic fantasy, but I read far less than I'd like. | ||
| − | + | <div class="lb"></div> | |
All in all, I had a wonderful summer and look forward to meeting everyone in Boston. So, I hope to see everyone there! | All in all, I had a wonderful summer and look forward to meeting everyone in Boston. So, I hope to see everyone there! | ||
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| + | $$(".team_members .card.flipped").each(function(v, k) | ||
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Revision as of 11:05, 31 October 2017
<!DOCTYPE html>
Comparison of endonuclease activity for Cas9 and Cpf1 that has been produced in, and excreted by, HEK293 cells.
MESA two-component system replication
Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.
OUTCASST system production
Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.
Modeling and
mathematics
mathematics
Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.
InterLab study participation
Results and details of our measurements for the iGEM 2017 InterLab Study.
Stakeholders & opinions
Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.
Risks & safety-issues
Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.
Design & integration
OUTCASST toolkit and product design with factors such as bio-safety and user-friendliness taken into account.
Outreach
Videos we made for the dutch public, together with 'de Kennis van Nu'.
Meet our team
About us, our interests and roles in the team and our supervisors.
Sponsors
A listing of our sponsors, how they assisted us and our gratitude for their assistance.
Collaborations
Read about our exchanges with other iGEM teams and government agencies.
Achievements
A short description of all that we have achieved during our participation in the iGEM.
Attributions
A thank-you for everyone that assited us, both in and outside the lab.
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