Cas9 & Cpf1 secretion
and activity
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<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
<span class="team-duties"> | <span class="team-duties"> | ||
| − | › Human Practices - Application/Safety/Design<br> | + | › Human Practices -<br> Application/Safety/Design<br> |
› Experimental - Assembly/InterLab Study<br> | › Experimental - Assembly/InterLab Study<br> | ||
› Funding & Sponsoring | › Funding & Sponsoring | ||
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<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
<span class="team-duties"> | <span class="team-duties"> | ||
| − | › Human Practices - Design/Safety/Application<br> | + | › Human Practices -<br> Design/Safety/Application<br> |
› InterLab study | › InterLab study | ||
</span> | </span> | ||
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<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
<span class="team-duties"> | <span class="team-duties"> | ||
| − | › Experimental - Cas9 & Cpf1 | + | › Experimental - Cas9 & Cpf1 secretion<br> and activity<br> |
› Funding & Sponsoring | › Funding & Sponsoring | ||
</span> | </span> | ||
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<span class="team-duties"> | <span class="team-duties"> | ||
› Experimental - MESA replication<br> | › Experimental - MESA replication<br> | ||
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<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
<span class="team-duties"> | <span class="team-duties"> | ||
| − | › Human Practices - Design/Safety/Application | + | › Human Practices -<br> Design/Safety/Application |
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<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
<span class="team-duties"> | <span class="team-duties"> | ||
| − | › Experimental - MESA replication/Assembly/Secreted Cpf1 and Cas9<br> | + | › Experimental - MESA<br> replication/Assembly/Secreted Cpf1<br> and Cas9<br> |
› Public Relations<br> | › Public Relations<br> | ||
› Funding & Sponsoring<br> | › Funding & Sponsoring<br> | ||
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<div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
<span class="team-duties"> | <span class="team-duties"> | ||
| − | › Experimental - MESA replication/Secreted Cpf1 and Cas9<br> | + | › Experimental - MESA<br> replication/Secreted Cpf1 and Cas9<br> |
› Funding & Sponsoring<br> | › Funding & Sponsoring<br> | ||
› Public Relations | › Public Relations | ||
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<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
<div class="team-bio" style="height: 61px;"> | <div class="team-bio" style="height: 61px;"> | ||
| − | Hi, I am Glenn (). I'm a student of Utrecht's Molecular & Cellular Life Science master-programme, currently doing a project at the Theoretical Biology group. I work on temperate phage decision-making by constructing an individual based model. Yes, most of my time is spent staring at a computer screen, wondering why code doesn't work. Small surprise; the group has me working on the wiki. | + | Hi, I am Glenn (22). I'm a student of Utrecht's Molecular & Cellular Life Science master-programme, currently doing a project at the Theoretical Biology group. I work on temperate phage decision-making by constructing an individual based model. Yes, most of my time is spent staring at a computer screen, wondering why code doesn't work. Small surprise; the group has me working on the wiki. |
<div class="lb"></div> | <div class="lb"></div> | ||
I did my BSc in Groningen and, honestly, I occasionally miss working in a lab, a void easily filled by participating in the iGEM. In the team, I tend to be the cynic who takes notes. Outside of work, I often find myself writing. I like stories, particularly short SF and heroic fantasy, but I read far less than I'd like. | I did my BSc in Groningen and, honestly, I occasionally miss working in a lab, a void easily filled by participating in the iGEM. In the team, I tend to be the cynic who takes notes. Outside of work, I often find myself writing. I like stories, particularly short SF and heroic fantasy, but I read far less than I'd like. | ||
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Revision as of 11:32, 31 October 2017
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Comparison of endonuclease activity for Cas9 and Cpf1 that has been produced in, and excreted by, HEK293 cells.
MESA two-component system replication
Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.
OUTCASST system production
Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.
Modeling and
mathematics
mathematics
Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.
InterLab study participation
Results and details of our measurements for the iGEM 2017 InterLab Study.
Stakeholders & opinions
Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.
Risks & safety-issues
Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.
Design & integration
OUTCASST toolkit and product design with factors such as bio-safety and user-friendliness taken into account.
Outreach
Videos we made for the dutch public, together with 'de Kennis van Nu'.
Meet our team
About us, our interests and roles in the team and our supervisors.
Sponsors
A listing of our sponsors, how they assisted us and our gratitude for their assistance.
Collaborations
Read about our exchanges with other iGEM teams and government agencies.
Achievements
A short description of all that we have achieved during our participation in the iGEM.
Attributions
A thank-you for everyone that assited us, both in and outside the lab.
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