Difference between revisions of "Team:ETH Zurich/Protocols"
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<summary>Chemical Transformation</summary> | <summary>Chemical Transformation</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" download>Download link</a> | ||
</details> | </details> | ||
Revision as of 13:16, 31 October 2017
Protocols
General Protocols
Chemical Transformation
Download linkColony PCR
Consumables
Cryostock of Bacteria
Electroporation
Gel Extraction
Gibson Assembly
Ligase Chain Reaction
Miniprep
PCR Clean-Up
Phusion PCR
Preparation of Chemically Competent Cells
Preparation of Electrocompetent Cells
Restriction Ligation
Tumor Sensor
Quorum Sensing End-Point Characterization
AND-gate Without Quorum Sensing
AND-gate With Quorum Sensing
MRI Contrast Agent
Fluorescence Measurement to Obtain the AHL Dose-Response Curve
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle
Anti-Cancer Toxin
Fluorescence Measurement to Obtain the AHL Dose-Response Curve
SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28
Heat Sensor
Heat-Induction of TlpA-Repressed GFP Expression
Cell Lysis
