Difference between revisions of "Team:ETH Zurich/Protocols"
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<summary>Chemical Transformation</summary> | <summary>Chemical Transformation</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="100%" height="700px"/> | ||
| − | <a href="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" download>Download | + | <a href="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" download>Download</a> |
</details> | </details> | ||
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<summary>Colony PCR</summary> | <summary>Colony PCR</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Consumables</summary> | <summary>Consumables</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Cryostock of Bacteria</summary> | <summary>Cryostock of Bacteria</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Electroporation</summary> | <summary>Electroporation</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Gel Extraction</summary> | <summary>Gel Extraction</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Gibson Assembly</summary> | <summary>Gibson Assembly</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Ligase Chain Reaction</summary> | <summary>Ligase Chain Reaction</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Miniprep</summary> | <summary>Miniprep</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>PCR Clean-Up</summary> | <summary>PCR Clean-Up</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Phusion PCR</summary> | <summary>Phusion PCR</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Preparation of Chemically Competent Cells</summary> | <summary>Preparation of Chemically Competent Cells</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Preparation of Electrocompetent Cells</summary> | <summary>Preparation of Electrocompetent Cells</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Restriction Ligation</summary> | <summary>Restriction Ligation</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download>Download</a> | ||
</details> | </details> | ||
</section> | </section> | ||
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<summary>Quorum Sensing End-Point Characterization</summary> | <summary>Quorum Sensing End-Point Characterization</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>AND-gate Without Quorum Sensing</summary> | <summary>AND-gate Without Quorum Sensing</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>AND-gate With Quorum Sensing</summary> | <summary>AND-gate With Quorum Sensing</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a> | ||
</details> | </details> | ||
</section> | </section> | ||
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<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary> | <summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary> | <summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <i>E. coli</i> Nissle</summary> | <summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <i>E. coli</i> Nissle</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary> | <summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a> | ||
</details> | </details> | ||
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<summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary> | <summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a> | ||
</details> | </details> | ||
</section> | </section> | ||
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<summary>Heat-Induction of TlpA-Repressed GFP Expression</summary> | <summary>Heat-Induction of TlpA-Repressed GFP Expression</summary> | ||
<embed src="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" width="100%" height="700px"/> | <embed src="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" download>Download</a> | ||
</details> | </details> | ||
</section> | </section> | ||
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<section> | <section> | ||
<h1>Cell Lysis</h1> | <h1>Cell Lysis</h1> | ||
| + | <details> | ||
| + | <summary>Heat-Induction of TlpA-Repressed GFP Expression</summary> | ||
| + | <embed src="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" width="100%" height="700px"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" download>Download</a> | ||
| + | </details> | ||
</section> | </section> | ||
Revision as of 13:21, 31 October 2017
Protocols
General Protocols
Chemical Transformation
DownloadColony PCR
DownloadConsumables
DownloadCryostock of Bacteria
DownloadElectroporation
DownloadGel Extraction
DownloadGibson Assembly
DownloadLigase Chain Reaction
DownloadMiniprep
DownloadPCR Clean-Up
DownloadPhusion PCR
DownloadPreparation of Chemically Competent Cells
DownloadPreparation of Electrocompetent Cells
DownloadRestriction Ligation
DownloadTumor Sensor
Quorum Sensing End-Point Characterization
DownloadAND-gate Without Quorum Sensing
DownloadAND-gate With Quorum Sensing
DownloadMRI Contrast Agent
Fluorescence Measurement to Obtain the AHL Dose-Response Curve
DownloadSDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin
DownloadMagnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle
DownloadAnti-Cancer Toxin
Fluorescence Measurement to Obtain the AHL Dose-Response Curve
DownloadSDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28
DownloadHeat Sensor
Heat-Induction of TlpA-Repressed GFP Expression
DownloadCell Lysis
Heat-Induction of TlpA-Repressed GFP Expression
Download
