Difference between revisions of "Team:NTHU Taiwan"

 
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Line 1: Line 1:
 +
{{:Team:NTHU_Taiwan/MenuBar}}
 
<html>
 
<html>
<head>
 
<title>NTHU iGEM</title>
 
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
 
<meta name="description" content="" />
 
<meta name="keywords" content="" />
 
<style  type="text/css">
 
@import url("https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css");
 
@import url("http://fonts.googleapis.com/css?family=Open+Sans:300,400,600,700,800");
 
  
 +
<style>
  
/* Basic */
+
.slider_container {
 +
}
  
body {
+
.slider_container div {
background: #222222;
+
    position: absolute;
}
+
    opacity: 0;
 +
    filter: alpha(opacity=0);
 +
}
 +
.slider_container div {
 +
    -webkit-animation: round 5s linear infinite;
 +
            animation: round 5s linear infinite;
 +
}
  
body.loading * {
+
@-webkit-keyframes round {
-moz-transition: none !important;
+
    20% {
-webkit-transition: none !important;
+
        opacity: 1;
-o-transition: none !important;
+
        filter: alpha(opacity=100);
-ms-transition: none !important;
+
        /* 0 - 1秒 淡入*/
transition: none !important;
+
    }
-moz-animation: none !important;
+
    40% {
-webkit-animation: none !important;
+
        opacity: 1;
-o-animation: none !important;
+
        filter: alpha(opacity=100);
-ms-animation: none !important;
+
        /* 1- 5秒靜止*/
animation: none !important;
+
    }
}
+
    80% {
 +
        opacity: 0;
 +
        filter: alpha(opacity=0);
 +
        /* 5-6秒淡出*/
 +
    }
 +
}
 +
@keyframes round {
 +
    20% {
 +
        opacity: 1;
 +
        filter: alpha(opacity=100);
 +
        /* 0 - 1秒 淡入*/
 +
    }
 +
    40% {
 +
        opacity: 1;
 +
        filter: alpha(opacity=100);
 +
        /* 1- 5秒靜止*/
 +
    }
 +
    80% {
 +
        opacity: 0;
 +
        filter: alpha(opacity=0);
 +
        /* 5-6秒淡出*/
 +
    }
 +
}
 +
.slider_container div:nth-child(2) {
 +
    -webkit-animation-delay: 0s;
 +
            animation-delay: 0s;
 +
}
  
body, input, select, textarea {
+
.slider_container div:nth-child(1) {
color: #555555;
+
    -webkit-animation-delay: 3s;
font-family: 'Open Sans', sans-serif;
+
            animation-delay: 3s;
font-size: 12pt;
+
}
font-weight: 300;
+
line-height: 1.65em;
+
}
+
  
a {
+
</style>
color: #82b440;
+
text-decoration: underline;
+
}
+
  
strong, b {
+
<div class="content">
font-weight: 700;
+
<div class="slider_container">
}
+
 
+
em, i {
+
font-style: italic;
+
}
+
 
+
p, ul, ol, dl, table, blockquote {
+
margin: 0 0 2em 0;
+
}
+
 
+
p {
+
line-height: 1.8em;
+
}
+
 
+
p.medium {
+
font-size: 1.4em;
+
}
+
 
+
h1, h2, h3, h4, h5, h6 {
+
color: inherit;
+
font-weight: 700;
+
}
+
 
+
h1 a, h2 a, h3 a, h4 a, h5 a, h6 a {
+
color: inherit;
+
text-decoration: none;
+
}
+
 
+
h2 {
+
font-size: 1.5em;
+
}
+
 
+
h3 {
+
font-size: 1.25em;
+
}
+
 
+
sub {
+
font-size: 0.8em;
+
position: relative;
+
top: 0.5em;
+
}
+
 
+
sup {
+
font-size: 0.8em;
+
position: relative;
+
top: -0.5em;
+
}
+
 
+
hr {
+
border-top: solid 1px #82b440;
+
border: 0;
+
margin-bottom: 1.5em;
+
}
+
 
+
blockquote {
+
border-left: solid 0.5em #82b440;
+
font-style: italic;
+
padding: 1em 0 1em 2em;
+
}
+
 
+
section.special, article.special {
+
text-align: center;
+
}
+
 
+
header.major {
+
padding-bottom: 3em;
+
text-align: center;
+
text-transform: uppercase;
+
}
+
 
+
header.major h2 {
+
font-size: 2.6em;
+
font-weight: 700;
+
}
+
 
+
header.major .byline {
+
display: block;
+
padding-top: 1em;
+
letter-spacing: 1px;
+
font-size: 1.4em;
+
}
+
 
+
footer > :last-child {
+
margin-bottom: 0;
+
}
+
 
+
footer.major {
+
padding-top: 3em;
+
}
+
 
+
/* Form */
+
 
+
input[type="text"],
+
input[type="password"],
+
input[type="email"],
+
textarea {
+
-moz-appearance: none;
+
-webkit-appearance: none;
+
-o-appearance: none;
+
-ms-appearance: none;
+
appearance: none;
+
background: none;
+
border: solid 1px #82b440;
+
color: inherit;
+
display: block;
+
outline: 0;
+
padding: 0.75em;
+
text-decoration: none;
+
width: 100%;
+
}
+
 
+
input[type="text"]:focus,
+
input[type="password"]:focus,
+
input[type="email"]:focus,
+
textarea:focus {
+
border-color: #82b440;
+
}
+
 
+
input[type="text"],
+
input[type="password"],
+
input[type="email"] {
+
line-height: 1em;
+
}
+
 
+
::-webkit-input-placeholder {
+
color: inherit;
+
opacity: 0.5;
+
position: relative;
+
top: 3px;
+
}
+
 
+
:-moz-placeholder {
+
color: inherit;
+
opacity: 0.5;
+
}
+
 
+
::-moz-placeholder {
+
color: inherit;
+
opacity: 0.5;
+
}
+
 
+
:-ms-input-placeholder {
+
color: inherit;
+
opacity: 0.5;
+
}
+
 
+
.formerize-placeholder {
+
color: rgba(85, 85, 85, 0.5) !important;
+
}
+
 
+
/* Image */
+
 
+
.image {
+
border: 0;
+
position: relative;
+
}
+
 
+
.image.fit {
+
display: block;
+
}
+
 
+
.image.fit img {
+
display: block;
+
width: 100%;
+
}
+
 
+
.image.feature {
+
display: block;
+
margin: 0 0 2em 0;
+
}
+
 
+
.image.feature img {
+
display: block;
+
width: 100%;
+
}
+
 
+
/* Icon */
+
 
+
.icon {
+
position: relative;
+
}
+
 
+
.icon:before {
+
content: "";
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
.icon > .label {
+
display: none;
+
}
+
 
+
/* Lists */
+
 
+
ol.default {
+
list-style: decimal;
+
padding-left: 1.25em;
+
}
+
 
+
ol.default li {
+
padding-left: 0.25em;
+
}
+
 
+
ul.default {
+
margin: 0;
+
padding: 0;
+
list-style: none;
+
}
+
 
+
ul.default li {
+
padding: 0.40em 0em;
+
}
+
 
+
ul.icons {
+
cursor: default;
+
padding-bottom: 2em;
+
}
+
 
+
ul.icons li {
+
display: inline-block;
+
line-height: 1em;
+
padding: 0.5em 1em;
+
}
+
 
+
ul.icons li:first-child {
+
padding-left: 0;
+
}
+
 
+
ul.icons li span {
+
display: none;
+
}
+
 
+
ul.icons li a {
+
text-decoration: none;
+
font-size: 2em;
+
color: inherit;
+
opacity: 0.2;
+
-moz-transition: all 0.35s ease-in-out;
+
-webkit-transition: all 0.35s ease-in-out;
+
-o-transition: all 0.35s ease-in-out;
+
-ms-transition: all 0.35s ease-in-out;
+
transition: all 0.35s ease-in-out;
+
}
+
 
+
ul.icons li a:hover {
+
color: inherit;
+
opacity: 1;
+
}
+
 
+
ul.actions {
+
cursor: default;
+
}
+
 
+
ul.actions:last-child {
+
margin-bottom: 0;
+
}
+
 
+
ul.actions li {
+
display: inline-block;
+
padding: 0 0 0 1.5em;
+
}
+
 
+
ul.actions li:first-child {
+
padding: 0;
+
}
+
 
+
ul.actions.vertical li {
+
display: block;
+
padding: 1.5em 0 0 0;
+
}
+
 
+
ul.actions.vertical li:first-child {
+
padding: 0;
+
}
+
 
+
ul.special-icons {
+
margin: 0em;
+
padding: 0em;
+
}
+
 
+
ul.special-icons > li {
+
position: relative;
+
padding: 0.50em 0em 0.50em 0em;
+
}
+
 
+
ul.special-icons > li:before {
+
position: absolute;
+
left: 0;
+
top: 2em;
+
display: block;
+
background: none;
+
font-size: 2em;
+
border-radius: 5px;
+
}
+
 
+
ul.special-icons > li:first-child {
+
border-top: none;
+
}
+
 
+
ul.special-icons h3 {
+
margin-bottom: 0.80em;
+
line-height: 2em;
+
text-transform: uppercase;
+
font-weight: 700;
+
font-size: 1.2em;
+
}
+
 
+
ul.special-icons span {
+
line-height: 190%;
+
}
+
 
+
ul.special-icons .fa {
+
float: left;
+
display: inline-block;
+
padding-right: 1em;
+
font-size: 1.4em;
+
color: #82b440;
+
}
+
 
+
ul.special-icons p {
+
padding-left: 2.7em;
+
}
+
 
+
/* Tables */
+
 
+
table {
+
width: 100%;
+
}
+
 
+
table.default {
+
width: 100%;
+
}
+
 
+
table.default tbody tr {
+
border-bottom: solid 1px #82b440;
+
}
+
 
+
table.default td {
+
padding: 0.5em 1em 0.5em 1em;
+
}
+
 
+
table.default th {
+
font-weight: 700;
+
padding: 0.5em 1em 0.5em 1em;
+
text-align: left;
+
}
+
 
+
table.default thead {
+
background: #555555;
+
color: #fff;
+
}
+
 
+
/* Button */
+
 
+
input[type="submit"],
+
input[type="reset"],
+
input[type="button"],
+
.button {
+
-moz-appearance: none;
+
-webkit-appearance: none;
+
-o-appearance: none;
+
-ms-appearance: none;
+
appearance: none;
+
background: none;
+
border-radius: none;
+
border: 2px solid;
+
border-color: #82b440;
+
color: #82b440;
+
cursor: pointer;
+
display: inline-block;
+
padding: 0.90em 1.2em;
+
letter-spacing: 1px;
+
text-align: center;
+
text-decoration: none;
+
text-transform: uppercase;
+
font-size: 1.4em;
+
-moz-transition: all 0.35s ease-in-out;
+
-webkit-transition: all 0.35s ease-in-out;
+
-o-transition: all 0.35s ease-in-out;
+
-ms-transition: all 0.35s ease-in-out;
+
transition: all 0.35s ease-in-out;
+
}
+
 
+
input[type="submit"]:hover,
+
input[type="reset"]:hover,
+
input[type="button"]:hover,
+
.button:hover {
+
background: #82b440;
+
color: white;
+
}
+
 
+
input[type="submit"].alt,
+
input[type="reset"].alt,
+
input[type="button"].alt,
+
.button.alt {
+
border-color: inherit;
+
color: inherit;
+
}
+
 
+
input[type="submit"].alt:hover,
+
input[type="reset"].alt:hover,
+
input[type="button"].alt:hover,
+
.button.alt:hover {
+
background: white;
+
color: #82b440;
+
}
+
 
+
input[type="submit"].fit,
+
input[type="reset"].fit,
+
input[type="button"].fit,
+
.button.fit {
+
width: 100%;
+
}
+
 
+
input[type="submit"].small,
+
input[type="reset"].small,
+
input[type="button"].small,
+
.button.small {
+
font-size: 0.8em;
+
}
+
 
+
/* Wrapper */
+
 
+
.wrapper {
+
padding: 6em 0em 4em 0em;
+
}
+
 
+
.wrapper.style1 {
+
padding: 0em;
+
background: #222222 url(../images/banner.jpg) no-repeat;
+
background-size: cover;
+
}
+
 
+
.wrapper.style2 {
+
background: #f2f2f2;
+
}
+
 
+
.wrapper.style2 .major {
+
text-align: left !important;
+
}
+
 
+
.wrapper.style2 .major h2 {
+
display: block;
+
margin-bottom: 0.70em;
+
letter-spacing: 1px;
+
line-height: 1.4em;
+
text-transform: uppercase;
+
font-size: 1.8em;
+
font-weight: 400;
+
}
+
 
+
.wrapper.style2 .major .byline {
+
letter-spacing: normal;
+
line-height: 1.6em;
+
text-transform: capitalize;
+
font-size: 1.4em;
+
}
+
 
+
.wrapper.style2 h3 {
+
display: block;
+
margin-bottom: 1em;
+
letter-spacing: 1px;
+
line-height: 1.4em;
+
text-transform: uppercase;
+
font-size: 1.6em;
+
font-weight: 400;
+
}
+
 
+
.wrapper.style3 {
+
padding-bottom: 6em;
+
background: #82b440;
+
text-align: center;
+
color: white;
+
}
+
 
+
.wrapper.style3 .container {
+
padding-left: 6em;
+
padding-right: 6em;
+
}
+
 
+
.wrapper.style3 p {
+
font-size: 1.6em;
+
}
+
 
+
.wrapper.style4 {
+
background: white;
+
}
+
 
+
.wrapper.style5 {
+
background: #82b440;
+
text-align: center;
+
color: white;
+
}
+
 
+
.wrapper.style5 .image {
+
display: block;
+
width: 60%;
+
margin: 0em auto 2em auto;
+
}
+
 
+
.wrapper.style5 .image img {
+
border-radius: 50%;
+
border: 10px solid;
+
border-color: white;
+
}
+
 
+
/* Header */
+
 
+
#header {
+
color: white;
+
height: 4em;
+
background: rgba(0, 0, 0, 0.5);
+
}
+
 
+
#header .container {
+
position: relative;
+
}
+
 
+
.homepage #logo {
+
display: none;
+
}
+
 
+
.homepage #nav {
+
position: static;
+
right: none;
+
text-align: center;
+
}
+
 
+
#logo {
+
line-height: 2em;
+
letter-spacing: 2px;
+
text-transform: uppercase;
+
font-size: 2em;
+
font-weight: 400;
+
}
+
 
+
#logo h1 {
+
display: inline-block;
+
margin: 0;
+
padding: 0;
+
}
+
 
+
#logo a {
+
color: inherit;
+
}
+
 
+
#nav {
+
position: absolute;
+
top: 0;
+
right: 0;
+
}
+
 
+
#nav > ul {
+
margin: 0;
+
}
+
 
+
#nav > ul > li {
+
border-radius: 4px;
+
display: inline-block;
+
margin-left: 0.5em;
+
padding: 0 0.5em;
+
}
+
 
+
#nav > ul > li a {
+
color: inherit;
+
line-height: 4em;
+
letter-spacing: 2px;
+
text-decoration: none;
+
text-transform: uppercase;
+
font-weight: 400;
+
font-size: 1em;
+
}
+
 
+
#nav > ul > li:first-child {
+
margin-left: 0;
+
}
+
 
+
#nav > ul > li.active a {
+
color: white;
+
}
+
 
+
#nav > ul > li > ul {
+
display: none;
+
}
+
 
+
/* Dropotron */
+
 
+
.dropotron {
+
top: 2em;
+
background: rgba(32, 32, 32, 0.75);
+
border-radius: 4px;
+
color: inherit;
+
min-width: 12em;
+
padding: 1em 0;
+
color: white;
+
}
+
 
+
.dropotron > li {
+
line-height: 2em;
+
padding: 0 1em;
+
}
+
 
+
.dropotron > li > a {
+
color: inherit;
+
letter-spacing: 2px;
+
text-decoration: none;
+
text-transform: uppercase;
+
}
+
 
+
.dropotron > li.active > a, .dropotron > li:hover > a {
+
color: inherit;
+
}
+
 
+
.dropotron.level-0 {
+
border-radius: 0 0 4px 4px;
+
font-size: 1em;
+
margin-left: -0.5em;
+
}
+
 
+
/* Banner */
+
 
+
#banner {
+
padding: 6em 0em 3em 0em;
+
text-align: center;
+
text-transform: uppercase;
+
color: white;
+
}
+
 
+
#banner .container {
+
padding: 0em 8em;
+
}
+
 
+
#banner h2 {
+
display: inline-block;
+
padding: 0.50em 0.30em;
+
background: #82b440;
+
font-size: 3em;
+
font-weight: 400;
+
}
+
 
+
#banner span, #banner p {
+
display: block;
+
letter-spacing: 1px;
+
text-transform: uppercase;
+
font-size: 1.6em;
+
font-weight: 300;
+
}
+
 
+
#banner span {
+
padding: 1em 0em;
+
}
+
 
+
/* Main */
+
 
+
#main {
+
padding: 4em 0;
+
}
+
 
+
#main #content .major, #main #sidebar .major {
+
text-align: left;
+
}
+
 
+
#main #sidebar section {
+
margin-top: 4em;
+
}
+
 
+
#main #sidebar section:first-child {
+
margin-top: 0;
+
}
+
 
+
#main #sidebar .major {
+
padding-bottom: 2em;
+
}
+
 
+
#main #sidebar .major h2 {
+
font-size: 1.8em;
+
}
+
 
+
/* Footer */
+
 
+
#footer {
+
padding: 6em 0 2em 0;
+
text-align: center;
+
color: white;
+
}
+
 
+
#footer hr {
+
border-bottom: 1px solid;
+
border-color: inherit;
+
opacity: .05;
+
}
+
 
+
#footer .major h2 {
+
color: inherit;
+
}
+
 
+
#footer .major .byline {
+
color: inherit;
+
opacity: .4;
+
}
+
 
+
#footer .copyright {
+
margin-top: 3em;
+
text-align: center;
+
}
+
 
+
/* Copyright */
+
 
+
#copyright {
+
position: relative;
+
text-transform: uppercase;
+
text-align: center;
+
padding: 3em 0em 3em 0em;
+
color: inherit;
+
opacity: .40;
+
}
+
 
+
#copyright a {
+
text-decoration: none;
+
color: inherit;
+
}
+
 
+
/* Extra */
+
 
+
#extra1 h2 {
+
display: block;
+
margin-bottom: 1em;
+
letter-spacing: 1px;
+
line-height: 1.4em;
+
text-transform: uppercase;
+
font-size: 1.8em;
+
font-weight: 400;
+
}
+
 
+
#extra1 h3 {
+
display: block;
+
margin-bottom: 1em;
+
letter-spacing: 1px;
+
line-height: 1.4em;
+
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<li class="active"><a href="index.html">HOME</a></li>
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<a href="">TEAM</a>
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<ul>
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<li><a href="#">Team</a></li>
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<li><a href="#">Collaborations</a></li>
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</ul>
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</li>
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<li>
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<a href="">PROJECT</a>
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<ul>
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<li><a href="#">Description</a></li>
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<li><a href="#">Design</a></li>
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<li><a href="#">Experiments</a></li>
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<li><a href="#">Notebook</a></li>
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<li><a href="#">Silver HP</a></li>
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<li><a href="#">Integrated and Gold</a></li>
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<li><a href="#">Applied Design</a></li>
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<span>NTHU_Taiwan</span>
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<section class="container">
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<div class="row double">
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<div class="12u">
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<h3>Project Introduction</h3>
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<p>This year, our goal is to deal with EDCs (Endocrine Disruptor Chemicals), which can interfere with endocrine systems, causing cancerous tumors, birth defects, and other developmental disorders. These chemicals are mostly man-made and found in various materials such as pesticides, food containers and personal care products. In this project, we primarily focus on two common kinds of EDCs, BPA (Bisphenol A) and NP (Nonyl Phenol), in industrial waste water.</p>
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<p>There are mainly two objectives in our project, detection of EDCs and degradation of EDCs.</p>
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<p>For detection, we first modified E.coli to express EDC receptor, ER (estrogen receptor alpha), on the membrane of E.coli. Next, we modified E.coli to produce monobodies and assembled them on a gold electrode surface. Once EDCs are binded with ER, monobodies will capture bounded ER, making E.coli closer to gold electrode. This leads to impedance changes on gold electrode. Via electrochemical impedance spectroscopy (EIS), we can measure this change and further estimate the concentration of EDCs.</p>
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<p>For degradation, we modified E.coli to produce target enzymes which are able to degrade BPA and NP. To make our project more applicable, we integrated target enzymes with activated carbon, membrane and bioreactor system to eliminate these contaminants in waste water. We also employed feedback control loop, together with detection of EDCs, so as to enhance the efficiency of degradation.</p>
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<p>In the future, we hope to put our idea into practice in order to make our world a better place.</p>
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Latest revision as of 13:33, 31 October 2017

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