|
|
| (390 intermediate revisions by 9 users not shown) |
| Line 1: |
Line 1: |
| | | | |
| − | {{Freiburg/CSS}}
| |
| − | {{Freiburg/Navbar}}
| |
| − |
| |
| − | <html>
| |
| − | <head>
| |
| − | <link href="https://fonts.googleapis.com/css?family=Josefin+Sans:100" rel="stylesheet">
| |
| − | <link rel="stylesheet" type="text/css"
| |
| − | href="https://2017.igem.org/Template:Team:Freiburg/CSS?action=raw&ctype=text/css"/>
| |
| − | <link rel="stylesheet" type="text/css"
| |
| − | href="https://2017.igem.org/Template:Team:Freiburg/CSS:bootstrap?action=raw&ctype=text/css"/>
| |
| − | <script type="text/javascript" src="https://2017.igem.org/Template:Team:Freiburg/JS:bootstrap?action=raw&ctype=text/javascript"></script>
| |
| − |
| |
| − | <style>
| |
| − | font-family: 'Josefin Sans', serif;
| |
| − |
| |
| − | .item-methods {
| |
| − | background-color: white;
| |
| − | width:500;
| |
| − | height: auto;
| |
| − | margin: 10px;
| |
| − | overflow: hidden;
| |
| − | }
| |
| − | .item-methods p{
| |
| − | font-family: 'Roboto', sans-serif!important;
| |
| − | font-size: 18px!important;
| |
| − | overflow: visible;
| |
| − | }
| |
| − | .item-methods1 {
| |
| − | background-color: white;
| |
| − | width:500;
| |
| − | height: auto;
| |
| − | margin: 10px;
| |
| − | padding-left: 50px;
| |
| − | overflow: hidden;
| |
| − | }
| |
| − | .item-methods1 p{
| |
| − | font-family: 'Roboto', sans-serif!important;
| |
| − | font-size: 18px!important;
| |
| − | overflow: visible;
| |
| − | }
| |
| − | p.italic {
| |
| − | font-family: 'Roboto', sans-serif;
| |
| − | font-style: italic;
| |
| − | font-weight: 300;
| |
| − | }
| |
| − |
| |
| − |
| |
| − | pfeil{
| |
| − | text-align: center;
| |
| − | color: black;
| |
| − | }
| |
| − |
| |
| − | </style>
| |
| − | </head>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <body>
| |
| − | <div class="container">
| |
| − | <div class="row">
| |
| − | <div class="col-md-12 text-center">
| |
| − | <div class="flex-container">
| |
| − |
| |
| − | <h1 align="center">Cell Culture</h1>
| |
| − |
| |
| − | <div class="item-methods">
| |
| − | <h2>Transfection</h2>
| |
| − | <p>Mammalian cell transfection is used to introduce foreign nucleic acids into cells. Depending on cell line and desired effect, there are different ways to transfect mammalian cells. These methods can be broadly classified into two groups: transient and stable transfections. Normally, a selection marker is needed to identify cells that are transfected.</p>
| |
| − | </div>
| |
| − | <div class="item-methods">
| |
| − | <h2>Transient Transfection</h2>
| |
| − | <p>Transient transfections generate short-term impact of alterations in gene and protein expression. In this case, the expression of the introduced gene is temporary because the foreign nucleic acids are not integrated into the host cell genome and therefore will not be passed on by cell division. Transient transfection of cells was achieved by two different methods:
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="item-methods1">
| |
| − | <h2>PEI transfection</h2>
| |
| − | <p>Polyethylenimine (PEI) is used as a polymeric transfection. PEI condenses DNA into positively charged particles, which bind to anionic cell surface residues and are brought into the cell via endocytosis. Using this method transient transfected HEK cells were generated.</p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="item-methods1">
| |
| − | <h2>Electroporation</h2>
| |
| − | <p>Electroporation is a method, which is used to introduce target genes into cells transiently in a physical way. To achieve this, an electrical field is generated around the cells to increase the permeability of the cell membrane, so that DNA can enter the cells. During our project we used electroporation to generate transient transfected Jurkat cells.</p>
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </body>
| |
| − |
| |
| − |
| |
| − | </html>
| |
| − |
| |
| − | {{Freiburg/Footer}}
| |
