Difference between revisions of "Team:ETH Zurich/Protocols"

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<summary>Chemical Transformation</summary>
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<a download="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf">Download</a>
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<summary>Colony PCR</summary>
 
<summary>Colony PCR</summary>
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<a href="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" download>Download</a>
 
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<summary>Consumables</summary>
 
<summary>Consumables</summary>
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<a href="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" download>Download</a>
 
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<summary>Cryostock of Bacteria</summary>
 
<summary>Cryostock of Bacteria</summary>
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<a href="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" download>Download</a>
 
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<summary>Electroporation</summary>
 
<summary>Electroporation</summary>
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<a href="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" download>Download</a>
 
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<summary>Gel Extraction</summary>
 
<summary>Gel Extraction</summary>
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<summary>Gibson Assembly</summary>
 
<summary>Gibson Assembly</summary>
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<a href="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" download>Download</a>
 
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<summary>Ligase Chain Reaction</summary>
 
<summary>Ligase Chain Reaction</summary>
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<summary>Miniprep</summary>
 
<summary>Miniprep</summary>
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<summary>PCR Clean-Up</summary>
 
<summary>PCR Clean-Up</summary>
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<a href="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" download>Download</a>
 
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<summary>Phusion PCR</summary>
 
<summary>Phusion PCR</summary>
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<a href="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" download>Download</a>
 
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<summary>Preparation of Chemically Competent Cells</summary>
 
<summary>Preparation of Chemically Competent Cells</summary>
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<summary>Preparation of Electrocompetent Cells</summary>
 
<summary>Preparation of Electrocompetent Cells</summary>
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<a href="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" download>Download</a>
 
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<summary>Restriction Ligation</summary>
 
<summary>Restriction Ligation</summary>
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<a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download>Download</a>
 
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<summary>Quorum Sensing End-Point Characterization</summary>
 
<summary>Quorum Sensing End-Point Characterization</summary>
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<summary>AND-gate Without Quorum Sensing</summary>
 
<summary>AND-gate Without Quorum Sensing</summary>
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<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
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<summary>AND-gate With Quorum Sensing</summary>
 
<summary>AND-gate With Quorum Sensing</summary>
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<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
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<summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary>
 
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary>
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<summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <span class="bacterium">E. coli</span> Nissle</summary>
 
<summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <span class="bacterium">E. coli</span> Nissle</summary>
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<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
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<summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary>
 
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary>
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<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
 
<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
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<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>
 
<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>
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<a href="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" download>Download</a>
 
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Revision as of 14:21, 31 October 2017

/Head_N_Prefix}}

Protocols

General Protocols

Chemical Transformation
Colony PCR Download
Consumables Download
Cryostock of Bacteria Download
Electroporation Download
Gel Extraction Download
Gibson Assembly Download
Ligase Chain Reaction Download
Miniprep Download
PCR Clean-Up Download
Phusion PCR Download
Preparation of Chemically Competent Cells Download
Preparation of Electrocompetent Cells Download
Restriction Ligation Download

Tumor Sensor

Quorum Sensing End-Point Characterization Download
AND-gate Without Quorum Sensing Download
AND-gate With Quorum Sensing Download

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin Download
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle Download

Anti-Cancer Toxin

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28 Download

Heat Sensor

Heat-Induction of TlpA-controlled GFP Expression Download

Cell Lysis

Heat-Induction of GFP Release by TlpA-controlled Protein E Expression Download