Difference between revisions of "Team:ETH Zurich/Protocols"

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<h1>General Protocols</h1>
 
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<a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download>Download</a>
 
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<h1>Tumor Sensor</h1>
 
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<summary>Quorum Sensing End-Point Characterization</summary>
 
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<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
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<h1>MRI Contrast Agent</h1>
 
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<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 
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<a href="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" download>Download</a>
 
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<h1>Anti-Cancer Toxin</h1>
 
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<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 
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<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 
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<h1>Heat Sensor</h1>
 
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<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
 
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<a href="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" download>Download</a>
 
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<h1>Cell Lysis</h1>
 
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<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>
 
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<a href="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" download>Download</a>
 
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Revision as of 14:27, 31 October 2017

Protocols

General Protocols

Chemical Transformation
Colony PCR Download
Consumables Download
Cryostock of Bacteria Download
Electroporation Download
Gel Extraction Download
Gibson Assembly Download
Ligase Chain Reaction Download
Miniprep Download
PCR Clean-Up Download
Phusion PCR Download
Preparation of Chemically Competent Cells Download
Preparation of Electrocompetent Cells Download
Restriction Ligation Download

Tumor Sensor

Quorum Sensing End-Point Characterization Download
AND-gate Without Quorum Sensing Download
AND-gate With Quorum Sensing Download

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin Download
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle Download

Anti-Cancer Toxin

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28 Download

Heat Sensor

Heat-Induction of TlpA-controlled GFP Expression Download

Cell Lysis

Heat-Induction of GFP Release by TlpA-controlled Protein E Expression Download