Difference between revisions of "Team:ETH Zurich/Protocols"

(Fix width of first pdf.)
(Add class to summaries.)
Line 13: Line 13:
 
<h1>General Protocols</h1>
 
<h1>General Protocols</h1>
  
−
<div class="multi-summary">
+
<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Chemical Transformation</summary>
 
<summary>Chemical Transformation</summary>
Line 101: Line 101:
 
<section>
 
<section>
 
<h1>Tumor Sensor</h1>
 
<h1>Tumor Sensor</h1>
−
<div class="multi-summary">
+
<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Quorum Sensing End-Point Characterization</summary>
 
<summary>Quorum Sensing End-Point Characterization</summary>
Line 124: Line 124:
 
<section>
 
<section>
 
<h1>MRI Contrast Agent</h1>
 
<h1>MRI Contrast Agent</h1>
−
<div class="multi-summary">
+
<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
Line 147: Line 147:
 
<section>
 
<section>
 
<h1>Anti-Cancer Toxin</h1>
 
<h1>Anti-Cancer Toxin</h1>
−
<div class="multi-summary">
+
<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
Line 164: Line 164:
 
<section>
 
<section>
 
<h1>Heat Sensor</h1>
 
<h1>Heat Sensor</h1>
−
<div class="multi-summary">
+
<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
 
<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
Line 175: Line 175:
 
<section>
 
<section>
 
<h1>Cell Lysis</h1>
 
<h1>Cell Lysis</h1>
−
<div class="multi-summary">
+
<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>
 
<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>

Revision as of 14:32, 31 October 2017

Protocols

General Protocols

Chemical Transformation
Colony PCR Download
Consumables Download
Cryostock of Bacteria Download
Electroporation Download
Gel Extraction Download
Gibson Assembly Download
Ligase Chain Reaction Download
Miniprep Download
PCR Clean-Up Download
Phusion PCR Download
Preparation of Chemically Competent Cells Download
Preparation of Electrocompetent Cells Download
Restriction Ligation Download

Tumor Sensor

Quorum Sensing End-Point Characterization Download
AND-gate Without Quorum Sensing Download
AND-gate With Quorum Sensing Download

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin Download
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle Download

Anti-Cancer Toxin

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28 Download

Heat Sensor

Heat-Induction of TlpA-controlled GFP Expression Download

Cell Lysis

Heat-Induction of GFP Release by TlpA-controlled Protein E Expression Download