Difference between revisions of "Team:BNU-China"
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<html> | <html> | ||
| − | + | <head> | |
| − | + | <meta charset="utf-8"> | |
| − | + | <title>BNU-China</title> | |
| − | + | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | |
<meta name="Team" content="BNU-China" /> | <meta name="Team" content="BNU-China" /> | ||
| − | + | <meta name="keywords" content="iGEM 2017" /> | |
| − | + | <meta name="author" content="Ziyu Liu（刘梓钰）,Zhaodong Wang（王兆栋）,Xizong Zhang（张溪棕）"/> | |
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| + | <link type="text/css" rel="stylesheet" href="https://2017.igem.org/Template:BNU-China/CSS/final?action=raw&ctype=text/css | ||
| + | " /> | ||
<style> | <style> | ||
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| Line 26: | Line 28: | ||
#globalWrapper,#content{ | #globalWrapper,#content{ | ||
| − | + | width: 100%; | |
| − | + | height: 100%; | |
| − | + | border: 0px; | |
| − | + | margin: 0px; | |
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} | } | ||
#HQ_page p { | #HQ_page p { | ||
| − | + | text-align: justify!important; | |
| + | } | ||
| + | |||
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} | } | ||
| Line 42: | Line 48: | ||
#content{ | #content{ | ||
| − | + | line-height:normal; | |
} | } | ||
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</style> | </style> | ||
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| − | + | var navFontSize; | |
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| − | + | $(this).append('<div style="position:absolute;display:inline;width:100%;left:0px;"><ul style="background-color:rgba(224,64,28,0.6);"><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Description ">Background</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Design ">Design</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Demonstrate">Results</a></li></ul></div>'); | |
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| − | + | ||
| − | + | ||
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var x=$("#eye").width()/2; | var x=$("#eye").width()/2; | ||
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$("#eye").css({"left":parseFloat(String((e.pageX-x)/100)),"top":parseFloat(String((e.pageY-$(window).scrollTop()-y)/100))}); | $("#eye").css({"left":parseFloat(String((e.pageX-x)/100)),"top":parseFloat(String((e.pageY-$(window).scrollTop()-y)/100))}); | ||
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| + | <body style="background-color:rgb(247,237,183);"> | ||
| + | <div id="w_nav" style="top:15px;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/6/60/T--BNU-China--index2.1.png" alt="Sorry, the image is not spupported by your browser."> | ||
<img id="eye" src="https://static.igem.org/mediawiki/2017/5/53/T--BNU-China--index1.jpg" alt="Sorry, the image is not spupported by your browser." style="width:100;position:absolute;top:-3px;"> | <img id="eye" src="https://static.igem.org/mediawiki/2017/5/53/T--BNU-China--index1.jpg" alt="Sorry, the image is not spupported by your browser." style="width:100;position:absolute;top:-3px;"> | ||
<ul> | <ul> | ||
| Line 211: | Line 279: | ||
<li id="w_Modeling"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model">Modeling</a></li> | <li id="w_Modeling"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model">Modeling</a></li> | ||
<li id="w_Practice"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP">Practice</a></li> | <li id="w_Practice"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP">Practice</a></li> | ||
| − | <li id="w_home"><a class="w_a" style="opacity:0;" href="https://2017.igem.org/Team:BNU-China | + | <li id="w_home"><a class="w_a" style="opacity:0;" href="https://2017.igem.org/Team:BNU-China">Home</a></li> |
<li id="w_Achievement"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Judging">Achievement</a></li> | <li id="w_Achievement"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Judging">Achievement</a></li> | ||
<li id="w_Lab"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Team">Lab</a></li> | <li id="w_Lab"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Team">Lab</a></li> | ||
<li id="w_Safety"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Safety">Safety</a></li> | <li id="w_Safety"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
| − | + | <div id="w_navMenu" style="text-align:right;"> | |
| − | + | <p>Menu▼</p> | |
| − | + | <ul id="w_navMenuContent"> | |
| − | + | <li id="w_Project"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Description">Project</a></li> | |
| − | + | <li id="w_Modeling"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model">Modeling</a></li> | |
| − | + | <li id="w_Practice"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP">Practice</a></li> | |
| − | + | <li id="w_Achievement"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Judging">Achievement</a></li> | |
| − | + | <li id="w_Lab"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Team">Lab</a></li> | |
| − | + | <li id="w_Safety"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Safety">Safety</a></li> | |
</ul> | </ul> | ||
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="w_content" style="text-align:center;"> | |
| − | + | <div id="story0" class="w_indexPhoto" style="z-index:210;margin: 14px 0px 0px 0px;"> | |
| − | + | <div id="abstract"> | |
| − | + | <h1 style="line-height:normal;color:rgb(247,237,183);">Abstract</h1> | |
| − | + | <p style="text-align:justify;"> | |
| − | + | Our project aims to improve the loading capacity of yeast surface display system by displaying microtubules and flagellar filaments, respectively, onto the yeast surface. To start with, by using the agglutinin system, we anchored subunits of fibrous polymers which were represented here by microtubulin subunits or FilC, on the cell wall of yeast. Since the particular polymers' self-assembly could take place outside the cell, we provided the engineered yeast with an environment rich of pre-secreted polymers’ subunits. In this way, an extracellular tridimensional display system could be formed, which enlarged the loading capacity by roughly two orders of magnitude. Moreover, we remodified the structure of FliC, substituting its D3 domain by an enzyme of interest. By this means of displaying a fusion prtein of FilC-enzymes, the co-display ability was significantly enhanced. As a result, a highly efficient, whole-cell biocatalyst system was established. | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div id="story1" class="w_indexPhoto" style="z-index:209;margin: 14px 0px 0px 0px;"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/7/7e/T--BNU-China--story1.jpg" alt="Sorry, the image is not spupported by your browser."> | |
| − | + | </div> | |
| − | + | <div id="story2" class="w_indexPhoto" style="z-index:208;margin: 14px 0px 0px 0px;"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/0/07/T--BNU-China--story2.jpg" alt="Sorry, the image is not spupported by your browser."> | |
| − | + | </div> | |
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Latest revision as of 14:48, 31 October 2017
Abstract
Our project aims to improve the loading capacity of yeast surface display system by displaying microtubules and flagellar filaments, respectively, onto the yeast surface. To start with, by using the agglutinin system, we anchored subunits of fibrous polymers which were represented here by microtubulin subunits or FilC, on the cell wall of yeast. Since the particular polymers' self-assembly could take place outside the cell, we provided the engineered yeast with an environment rich of pre-secreted polymers’ subunits. In this way, an extracellular tridimensional display system could be formed, which enlarged the loading capacity by roughly two orders of magnitude. Moreover, we remodified the structure of FliC, substituting its D3 domain by an enzyme of interest. By this means of displaying a fusion prtein of FilC-enzymes, the co-display ability was significantly enhanced. As a result, a highly efficient, whole-cell biocatalyst system was established.
