Difference between revisions of "Team:BNU-China"
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<link type="text/css" rel="stylesheet" href="https://2017.igem.org/Template:BNU-China/CSS/final?action=raw&ctype=text/css | <link type="text/css" rel="stylesheet" href="https://2017.igem.org/Template:BNU-China/CSS/final?action=raw&ctype=text/css | ||
" /> | " /> | ||
| − | |||
<style> | <style> | ||
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear, .center {display: none;} /*-- hides default wiki settings --*/ | #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear, .center {display: none;} /*-- hides default wiki settings --*/ | ||
| Line 38: | Line 37: | ||
#HQ_page p { | #HQ_page p { | ||
text-align: justify!important; | text-align: justify!important; | ||
| + | } | ||
| + | |||
| + | #w_navMenu>p { | ||
| + | text-align: right!important; | ||
} | } | ||
| Line 67: | Line 70: | ||
var result767=window.matchMedia('(max-width: 767px)'); | var result767=window.matchMedia('(max-width: 767px)'); | ||
$(document).ready(function(){ | $(document).ready(function(){ | ||
| + | $("#w_Project").hover(function(){ | ||
| + | $(this).append('<div style="position:absolute;display:inline;width:100%;left:0px;"><ul style="background-color:rgba(224,64,28,0.6);"><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Description ">Background</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Design ">Design</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Demonstrate">Results</a></li></ul></div>'); | ||
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| + | },function(){ | ||
| + | $("#w_nav>ul>li>div").remove(); | ||
| + | }); | ||
| + | $("#w_Modeling").hover(function(){ | ||
| + | $(this).append('<div style="position:absolute;display:inline;width:100%;left:0px;"><ul style="background-color:rgba(224,64,28,0.6);"><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model">Overview</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model/microtubule">Microtubule</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model/flagellin">Flagellar Filament</a></li></ul></div>'); | ||
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| + | },function(){ | ||
| + | $("#w_nav>ul>li>div").remove(); | ||
| + | }); | ||
| + | $("#w_Practice").hover(function(){ | ||
| + | $(this).append('<div style="position:absolute;display:inline;width:100%;left:0px;"><ul style="background-color:rgba(224,64,28,0.6);"><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP">Overview</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP/Silver">Silver</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP/Gold_Integrated">Gold</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Engagement">Engagement</a></li></ul></div>'); | ||
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| + | },function(){ | ||
| + | $("#w_nav>ul>li>div").remove(); | ||
| + | }); | ||
| + | $("#w_Achievement").hover(function(){ | ||
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| + | },function(){ | ||
| + | $("#w_nav>ul>li>div").remove(); | ||
| + | }); | ||
| + | $("#w_Lab").hover(function(){ | ||
| + | $(this).append('<div style="position:absolute;display:inline;width:100%;left:0px;"><ul style="background-color:rgba(224,64,28,0.6);"><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Team">Team</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Notebook">Notebook</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Experiments">Protocol</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Attributions">Attributions</a></li><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Acknowlegement">Acknowlegement</a></li></ul></div>'); | ||
| + | $("#w_nav>ul>li>div").css({"paddingTop":-1*parseFloat($("#w_nav>ul").css("top"))}); | ||
| + | },function(){ | ||
| + | $("#w_nav>ul>li>div").remove(); | ||
| + | }); | ||
| + | $("#w_Safety").hover(function(){ | ||
| + | $(this).append('<div style="position:absolute;display:inline;width:100%;left:0px;"><ul style="background-color:rgba(224,64,28,0.6);"><li><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Safety">Safety</a></li></ul></div>'); | ||
| + | $("#w_nav>ul>li>div").css({"paddingTop":-1*parseFloat($("#w_nav>ul").css("top"))}); | ||
| + | },function(){ | ||
| + | $("#w_nav>ul>li>div").remove(); | ||
| + | }); | ||
$("#top_title").hide(); | $("#top_title").hide(); | ||
$("#sideMenu").hide(); | $("#sideMenu").hide(); | ||
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$("#story0").show(); | $("#story0").show(); | ||
} | } | ||
| − | + | var fDownArrow=function(){ | |
if(idStory<idStoryMax){ | if(idStory<idStoryMax){ | ||
$("html").animate({"scrollTop":$(document).height()-$(window).height()},400); | $("html").animate({"scrollTop":$(document).height()-$(window).height()},400); | ||
| Line 89: | Line 128: | ||
$("html,body").animate({"scrollTop":0},400,function(){ | $("html,body").animate({"scrollTop":0},400,function(){ | ||
}); | }); | ||
| − | if(idStory==idStoryMax) $("#storyArrow"). | + | if(idStory==idStoryMax){ |
| − | }); | + | $("#storyArrow>img").attr('src', "https://static.igem.org/mediawiki/2017/a/a9/T--BNU-China--upArrow.jpg"); |
| + | $("#storyArrow>img").attr('alt', "↑"); | ||
| + | $("#storyArrow").unbind("click"); | ||
| + | $("#storyArrow").attr('id', 'upArrow'); | ||
| + | $("#upArrow").bind("click",fUpArrow); | ||
| + | } | ||
| + | }); | ||
} | } | ||
| − | }); | + | } |
| + | $("#storyArrow").bind("click",fDownArrow); | ||
| + | |||
| + | var fUpArrow=function(){ | ||
| + | $("html,body").animate({"scrollTop":0},800); | ||
| + | idStory=0; | ||
| + | $("#story"+String(idStory)).slideDown(400,'swing',function(){ | ||
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| + | $("#upArrow>img").attr('src', "https://static.igem.org/mediawiki/2017/3/33/T--BNU-China--index3.jpg"); | ||
| + | $("#upArrow>img").attr('alt', "↓"); | ||
| + | $("#upArrow").unbind("click"); | ||
| + | $("#upArrow").attr('id', 'storyArrow'); | ||
| + | $("#storyArrow").bind("click",fDownArrow); | ||
| + | }); | ||
| + | } | ||
| + | |||
$("#storyArrow").hover(function(){ | $("#storyArrow").hover(function(){ | ||
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window.onmousewheel =0; | window.onmousewheel =0; | ||
$("html,body").animate({"scrollTop":0}); | $("html,body").animate({"scrollTop":0}); | ||
| − | $("#story"+String(idStory)).slideUp( | + | $("#story"+String(idStory)).slideUp(400,'swing',function(){window.onmousewheel = handleMouseScroll;}); |
idStory=idStory+1; | idStory=idStory+1; | ||
$("#story"+String(idStory)).show(); | $("#story"+String(idStory)).show(); | ||
| − | if(idStory==idStoryMax) $("#storyArrow"). | + | if(idStory==idStoryMax){ |
| + | $("#storyArrow>img").attr('src', 'https://static.igem.org/mediawiki/2017/a/a9/T--BNU-China--upArrow.jpg'); | ||
| + | $("#storyArrow>img").attr('alt', "↑"); | ||
| + | $("#storyArrow").unbind("click"); | ||
| + | $("#storyArrow").attr('id', 'upArrow'); | ||
| + | $("#upArrow").bind("click",fUpArrow); | ||
| + | } | ||
} | } | ||
} | } | ||
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$("html,body").animate({"scrollTop":0},800); | $("html,body").animate({"scrollTop":0},800); | ||
idStory=idStory-1; | idStory=idStory-1; | ||
| − | $("#story"+String(idStory)).slideDown( | + | $("#story"+String(idStory)).slideDown(400,'swing',function(){$("#story"+String(idStory+1)).hide();window.onmousewheel = handleMouseScroll;}); |
| + | } | ||
| + | if(idStory==idStoryMax-1){ | ||
| + | $("#upArrow>img").attr('src', 'https://static.igem.org/mediawiki/2017/3/33/T--BNU-China--index3.jpg'); | ||
| + | $("#upArrow>img").attr('alt', "↓"); | ||
| + | $("#upArrow").unbind("click"); | ||
| + | $("#upArrow").attr('id', 'storyArrow'); | ||
| + | $("#storyArrow").bind("click",fDownArrow); | ||
} | } | ||
| − | |||
} | } | ||
} | } | ||
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$("#storyArrow").hide(); | $("#storyArrow").hide(); | ||
} | } | ||
| − | else | + | else{ |
| − | $("#story0").height(document.body.scrollHeight-parseFloat($("#story0").css("padding-top"))-parseFloat($("#story0").css("top"))-parseFloat($("#story0").css("margin-top"))); | + | $("#story0").height(document.body.scrollHeight-parseFloat($("#story0").css("padding-top"))-parseFloat($("#story0").css("top"))-parseFloat($("#story0").css("margin-top"))+30); |
| + | } | ||
<!--导航栏自适应--> | <!--导航栏自适应--> | ||
if($(window).innerWidth()>=480){ | if($(window).innerWidth()>=480){ | ||
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$("#w_nav>div>ul").hide(); | $("#w_nav>div>ul").hide(); | ||
} | } | ||
| − | |||
$("#w_nav>ul").css({"top":$(window).width()/1280*(-41)}); | $("#w_nav>ul").css({"top":$(window).width()/1280*(-41)}); | ||
| − | $("#w_nav | + | $("#w_nav>ul>li>a").css({"width":$(window).width()/7-10,"font-size":$(window).width()/1280*25,"top":0}); |
navWidth=$(window).width(); | navWidth=$(window).width(); | ||
navFontSize=$("#w_nav>ul>li>a").css("font-size"); | navFontSize=$("#w_nav>ul>li>a").css("font-size"); | ||
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$("#w_nav>div").hide(); | $("#w_nav>div").hide(); | ||
$("#w_nav>ul").show(); | $("#w_nav>ul").show(); | ||
| − | $("#w_nav>ul>li>a").css({"width":$(window).width()/7-10,"font-size":parseFloat(navFontSize | + | $("#w_nav>ul>li>a").css({"width":$(window).width()/7-10,"font-size":parseFloat(navFontSize)*parseFloat(String($(window).width()))/parseFloat(String(navWidth))}); |
$("#w_nav>ul").css({"top":parseFloat(navTop)*parseFloat(String($(window).width()))/parseFloat(String(navWidth))}); | $("#w_nav>ul").css({"top":parseFloat(navTop)*parseFloat(String($(window).width()))/parseFloat(String(navWidth))}); | ||
| − | |||
navWidth=$(window).width(); | navWidth=$(window).width(); | ||
navFontSize=$("#w_nav>ul>li>a").css("font-size"); | navFontSize=$("#w_nav>ul>li>a").css("font-size"); | ||
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<li id="w_Modeling"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model">Modeling</a></li> | <li id="w_Modeling"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model">Modeling</a></li> | ||
<li id="w_Practice"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP">Practice</a></li> | <li id="w_Practice"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP">Practice</a></li> | ||
| − | <li id="w_home"><a class="w_a" style="opacity:0;" href="https://2017.igem.org/Team:BNU-China | + | <li id="w_home"><a class="w_a" style="opacity:0;" href="https://2017.igem.org/Team:BNU-China">Home</a></li> |
<li id="w_Achievement"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Judging">Achievement</a></li> | <li id="w_Achievement"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Judging">Achievement</a></li> | ||
<li id="w_Lab"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Team">Lab</a></li> | <li id="w_Lab"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Team">Lab</a></li> | ||
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<h1 style="line-height:normal;color:rgb(247,237,183);">Abstract</h1> | <h1 style="line-height:normal;color:rgb(247,237,183);">Abstract</h1> | ||
<p style="text-align:justify;"> | <p style="text-align:justify;"> | ||
| − | Our project aims to improve the loading capacity of yeast surface display system by displaying | + | Our project aims to improve the loading capacity of yeast surface display system by displaying microtubules and flagellar filaments, respectively, onto the yeast surface. To start with, by using the agglutinin system, we anchored subunits of fibrous polymers which were represented here by microtubulin subunits or FilC, on the cell wall of yeast. Since the particular polymers' self-assembly could take place outside the cell, we provided the engineered yeast with an environment rich of pre-secreted polymers’ subunits. In this way, an extracellular tridimensional display system could be formed, which enlarged the loading capacity by roughly two orders of magnitude. Moreover, we remodified the structure of FliC, substituting its D3 domain by an enzyme of interest. By this means of displaying a fusion prtein of FilC-enzymes, the co-display ability was significantly enhanced. As a result, a highly efficient, whole-cell biocatalyst system was established. |
</p> | </p> | ||
</div> | </div> | ||
Latest revision as of 14:48, 31 October 2017
Abstract
Our project aims to improve the loading capacity of yeast surface display system by displaying microtubules and flagellar filaments, respectively, onto the yeast surface. To start with, by using the agglutinin system, we anchored subunits of fibrous polymers which were represented here by microtubulin subunits or FilC, on the cell wall of yeast. Since the particular polymers' self-assembly could take place outside the cell, we provided the engineered yeast with an environment rich of pre-secreted polymers’ subunits. In this way, an extracellular tridimensional display system could be formed, which enlarged the loading capacity by roughly two orders of magnitude. Moreover, we remodified the structure of FliC, substituting its D3 domain by an enzyme of interest. By this means of displaying a fusion prtein of FilC-enzymes, the co-display ability was significantly enhanced. As a result, a highly efficient, whole-cell biocatalyst system was established.
