Difference between revisions of "Team:Tel-Hai/Notebook"

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<p class="text-center c-purple">Purple - working on improve BioBrick</p>
 
<p class="text-center c-purple">Purple - working on improve BioBrick</p>
 
<h4>27.8.17</h4>
 
<h4>27.8.17</h4>
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<p class="c-blue">S.cerevisiae 3095 with URA- mutation were obtained from Dr. Itay Onn (faculty of medicine of Bar Ilan University) and were isolated from YEPD plates.</p>
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<p class="c-orange">Isolation streak of Brett on selection medium- specific YEPD media.</p>
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<h4>29.8.17</h4>
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<p class="c-orange">Colonies from Brett isolation appeared. </p>
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<p class="c-orange">Colonies that appeared were tested using PCR with specific primers (from Prof. Martin Goldway lab) for Brett detection- PB2, RUX, DB, and NLS. We got negative results- no PCR product.</p>
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<img src="https://static.igem.org/mediawiki/2017/f/f4/T--Tel-Hai--dana-in-the-lab.JPG" alt="dana in the lab">
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<small>Fig.1- Dana having fun with Brett colonies in the lab</small>
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Revision as of 15:02, 31 October 2017

Notebook & Result

Red - working on RF cloning

Green - working on new genes

Blue - working on yeast cells

Orange - working on Brett

Purple - working on improve BioBrick

27.8.17

  • S.cerevisiae 3095 with URA- mutation were obtained from Dr. Itay Onn (faculty of medicine of Bar Ilan University) and were isolated from YEPD plates.

  • Isolation streak of Brett on selection medium- specific YEPD media.

29.8.17

  • Colonies from Brett isolation appeared.

  • Colonies that appeared were tested using PCR with specific primers (from Prof. Martin Goldway lab) for Brett detection- PB2, RUX, DB, and NLS. We got negative results- no PCR product.

    dana in the lab Fig.1- Dana having fun with Brett colonies in the lab

Tel-Hai