Difference between revisions of "Team:Tel-Hai/Notebook"

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<p class="text-center c-red">Red - working on RF cloning</p>
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<h4>30.8.17</h4>
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<p class="text-center c-green">Green - working on new genes</p>
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<p class="text-center c-blue">Blue - working on yeast cells</p>
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<p class="text-center c-orange">Orange - working on Brett</p>
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<p class="text-center c-purple">Purple - working on improve BioBrick</p>
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<h4>27.8.17</h4>
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<p class="c-blue">S.cerevisiae 3095 with URA- mutation were obtained from Dr. Itay Onn (faculty of medicine of Bar Ilan University) and were isolated from YEPD plates.</p>
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<p class="c-blue">We got pRS306/316 from Dr. Itay Onn.</p>
 
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<p class="c-orange">Isolation streak of Brett on selection medium- specific YEPD media.</p>
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<p class="c-blue">Transformation into TOP-10 competent cells:</p>
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<h4>29.8.17</h4>
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<p class="c-blue">Preparation of competent TOP10 e.coli bacteria for heat-shock transformation (we used this protocol – לתת לינק לדף פרוטוקול מספר 9)</p>
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<p class="c-orange">Colonies from Brett isolation appeared. </p>
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<p class="c-blue">T--Tel-Hai--dana-in-the-labTransformation for plasmids pRS306 + pRS316 into competent cells was performed using Heat-shock protocol then incubated O.N.</p>
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<p class="c-orange">Colonies that appeared were tested using PCR with specific primers (from Prof. Martin Goldway lab) for Brett detection- PB2, RUX, DB, and NLS. We got negative results- no PCR product.</p>
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<img src="https://static.igem.org/mediawiki/2017/f/f4/T--Tel-Hai--dana-in-the-lab.JPG" alt="dana in the lab">
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<small>Fig.1- Dana having fun with Brett colonies in the lab</small>
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Revision as of 15:13, 31 October 2017

Notebook & Result

30.8.17

  • We got pRS306/316 from Dr. Itay Onn.

  • Transformation into TOP-10 competent cells:

    • Preparation of competent TOP10 e.coli bacteria for heat-shock transformation (we used this protocol – לתת לינק לדף פרוטוקול מספר 9)

    • T--Tel-Hai--dana-in-the-labTransformation for plasmids pRS306 + pRS316 into competent cells was performed using Heat-shock protocol then incubated O.N.

Tel-Hai