Difference between revisions of "Team:Cardiff Wales/basicparts"

 
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<h1> Basic Parts </h1>
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<h1> Basic Parts used in our Project </h1>
 
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<p> Our team used several basic parts to create our gene constructs. Here is an overview  of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them. </p>
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<p align="center" style="background-color:#ffffff;" > <br><br>Our team used several basic parts to create our gene constructs. Here is an overview  of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.<br>Unfortunately we did not add part information to our <a href="https://igem.org/2017_Judging_Form?id=2404">Judging Form</a>, but our contributions are outlined below.<br><br><br> </p>
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  <tr>
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    <th>Registry link</th>
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    <th>Part</th>
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    <th>Plasmid</th>
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    <th>Summary</th>
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    <th>Length</th>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2404000">BBa_K2404000</a></td>
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    <td>TSH</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a> </td>
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    <td>A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does not</b> have His-tags for purification </td>
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<td>780bp</td>
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  </tr>
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  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2404001">BBa_K2404001</a></td>
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    <td>TSHH</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td> A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does</b> have His-tags for purification</td>
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<td>832bp</td>
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  </tr>
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  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2404002">BBa_K2404002</a></td>
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    <td>PDF1.2</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td>An inducible promoter that responds to the presence of jasmonic acid in the cell</td>
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<td>522bp</td>
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  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2404003">BBa_K2404003</a></td>
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    <td>PR2</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
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<td>519bp</td>
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  </tr>
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  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2404004">BBa_K2404004</a></td>
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    <td>GST6</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
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<td>519bp</td>
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  </tr>
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<tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2404005">BBa_K2404005</a></td>
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    <td>WRKY30</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td>An inducible promoter that responds to the presence of cellulose-derived oligomers<br><i>After submission to the registry we discovered that this promotor sequence was incorrect</i></td>
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<td>507bp</td>
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  </tr>
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  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2404006">BBa_K2404006</a></td>
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    <td>Luc+</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td>A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression. <i>Prior to submission to the registry we discovered that this coding sequence was incorrect so it was not submitted. In order to generate the level 1 clones containing LUC+ (<a href="http://parts.igem.org/Part:BBa_K2404013">BBa_K2404013</a>, <a href="http://parts.igem.org/Part:BBa_K2404014">BBa_K240414</a>) we used a previously characterised LUC+ protein that is contained within a different (not P10500) level 0 plasmid</i> </td>
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<td>1653bp</td>
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  </tr>
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  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_P10401">BBa_P10401</a></td>
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    <td>NosT</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td>A terminator from the nopaline synthase gene which is native to the  <i> Agrobacterium </i> tumor-inducing plasmid pTiT37</td>
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<td>285bp</td>
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  </tr>
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  <tr>
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        <td><a href="http://parts.igem.org/Part:BBa_P10000">BBa_P10000</a></td>
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    <td>LexA</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td>An estradiol inducible promoter from the LexA gene which is native to <i>Escherichia coli </i> </td>
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<td>362bp</td>
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  </tr>
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  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_P10003">BBa_P10003</a></td>
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    <td>35S-OTMV</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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    <td>A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus</td>
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<td>1446bp</td>
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  </tr>
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</table>
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Latest revision as of 15:30, 31 October 2017




Basic Parts used in our Project






Our team used several basic parts to create our gene constructs. Here is an overview of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.
Unfortunately we did not add part information to our Judging Form, but our contributions are outlined below.




Registry link Part Plasmid Summary Length
BBa_K2404000 TSH P10500 A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification 780bp
BBa_K2404001 TSHH P10500 A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification 832bp
BBa_K2404002 PDF1.2 P10500 An inducible promoter that responds to the presence of jasmonic acid in the cell 522bp
BBa_K2404003 PR2 P10500 An inducible promoter that responds to the presence of salicylic acid in the cell 519bp
BBa_K2404004 GST6 P10500 An inducible promoter that responds to the presence of salicylic acid in the cell 519bp
BBa_K2404005 WRKY30 P10500 An inducible promoter that responds to the presence of cellulose-derived oligomers
After submission to the registry we discovered that this promotor sequence was incorrect		 507bp
BBa_K2404006 Luc+ P10500 A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression. Prior to submission to the registry we discovered that this coding sequence was incorrect so it was not submitted. In order to generate the level 1 clones containing LUC+ (BBa_K2404013, BBa_K240414) we used a previously characterised LUC+ protein that is contained within a different (not P10500) level 0 plasmid 1653bp
BBa_P10401 NosT P10500 A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37 285bp
BBa_P10000 LexA P10500 An estradiol inducible promoter from the LexA gene which is native to Escherichia coli 362bp
BBa_P10003 35S-OTMV P10500 A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus 1446bp