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− | <img id="TopPicture" width="960" src="https://static.igem.org/mediawiki/2017/ | + | <img id="TopPicture" width="960" src="https://static.igem.org/mediawiki/2017/c/c2/T--Munich--FrontPagePicture_QuakeValve.jpg"> |
<table width="960" border=0 cellspacing=0 cellpadding=10> | <table width="960" border=0 cellspacing=0 cellpadding=10> | ||
<tr> | <tr> | ||
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<td colspan = 6 align=center valign=center> | <td colspan = 6 align=center valign=center> | ||
<p class="introduction"> | <p class="introduction"> | ||
− | We envision a portable,fully automated, fluidic device that can process biological samples in the field. We considered that every part of our device that gets in contact with our sample needs to be disposable and replaceable. Therefore we designed a replaceable fluidic chip out of PDMS that can be controlled via an external device. | + | We envision a portable, fully automated, fluidic device that can process biological samples in the field. We considered that every part of our device that gets in contact with our sample needs to be disposable and replaceable. Therefore we designed a replaceable fluidic chip out of PDMS that can be controlled via an external device. |
</p> | </p> | ||
<p> | <p> | ||
− | To achieve this we build so called Quake valves that are controlled via externally applied air pressure. To pump fluids we use an air balloon which is a super cheap supply for low pressure. To control our Quake valves we use a bicycle tube as a cheap and refillable supply for air pressure up to 5 bar. We use electrically powered air valves to control the Quake valves and build an electric circuit to control the air valves with a microcontroller. The valves can be easily downscaled and require no special equipment for their manufacture. We constructed the Quake valves by using 3D printed negative via soft lithography. A detailed protocol for manufacturing macroscopic fluidic chips with 3D Printed negatives can be found <a class="myLink" href="https://2017.igem.org/Team:Munich/Protocols">here</a> at the subitem "Soft lithography". | + | To achieve this we build so called Quake valves<sup><a class="myLink" href="#ref_1">1</a></sup> that are controlled via externally applied air pressure. To pump fluids we use an air balloon which is a super cheap supply for low pressure. To control our Quake valves we use a bicycle tube as a cheap and refillable supply for air pressure up to 5 bar. We use electrically powered air valves to control the Quake valves and build an electric circuit to control the air valves with a microcontroller. The valves can be easily downscaled and require no special equipment for their manufacture. We constructed the Quake valves by using 3D printed negative via soft lithography. A detailed protocol for manufacturing macroscopic fluidic chips with 3D Printed negatives can be found <a class="myLink" href="https://2017.igem.org/Team:Munich/Protocols">here</a> at the subitem "Soft lithography". |
</p> | </p> | ||
<h3>Function and Composition of a Quake Valve</h3> | <h3>Function and Composition of a Quake Valve</h3> | ||
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</p> | </p> | ||
<p> | <p> | ||
− | An image of an operational quake valve can be seen in the image below. For better contrast, we filled the flow channel with | + | An image of an operational quake valve can be seen in the image below. For better contrast, we filled the flow channel with black ink and the pressure chamber with yellow inc.</p> |
<div class="captionPicture"> | <div class="captionPicture"> | ||
− | <img width = | + | <img width = 400 src="https://static.igem.org/mediawiki/2017/4/4e/T--Munich--Hardware_Valvereal.png"> |
<p> | <p> | ||
Operational Quake valve. | Operational Quake valve. | ||
</p> | </p> | ||
+ | </div> | ||
<h3>Control Circuit for the air valves</h3> | <h3>Control Circuit for the air valves</h3> | ||
<p> | <p> | ||
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</tr> | </tr> | ||
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+ | <tr><td colspan=6 align=center valign=center> | ||
+ | <h3>References</h3> | ||
+ | <p> | ||
+ | <ol style="text-align: left"> | ||
+ | <li id="ref_1">Unger, Marc A., et al. "Monolithic microfabricated valves and pumps by multilayer soft lithography." Science 288.5463 (2000): 113-116. | ||
+ | APA </li> | ||
+ | </ol> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
Latest revision as of 16:10, 31 October 2017
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