Difference between revisions of "Team:Harvard/Basic Parts"
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The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the <a href="https://salislab.net/software/"> Salis Lab RBS generator </a>. | The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the <a href="https://salislab.net/software/"> Salis Lab RBS generator </a>. | ||
| − | <h2>The RBS sequence was generated from a randomized library of RBSs using the <a href="https://salislab.net/software/">Salis Lab RBS generator</a>. Then, plasmids with desired RBS strengths were screened for and selected. Refer to our <a href="https://2017.igem.org/Team:Harvard/Protocols">protocol page</a> for details in procedure. </h2> | + | <h2>Design</h2> |
| + | The RBS sequence was generated from a randomized library of RBSs using the <a href="https://salislab.net/software/">Salis Lab RBS generator</a>. Then, plasmids with desired RBS strengths were screened for and selected. Refer to our <a href="https://2017.igem.org/Team:Harvard/Protocols">protocol page</a> for details in procedure. </h2> | ||
Revision as of 18:40, 31 October 2017
Parts
This year we submitted 5 new parts to the parts registry.
The parts feature varying strength RBS in front of csgG, the porous protein involved in the secretion of monomeric csgA in wild-type E. coli. This part can be stitched together with a standard promoter and compared with other similar parts with different RBSs to see some varying level of csgG expression.
Origins of Sequences
The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the Salis Lab RBS generator .Design
The RBS sequence was generated from a randomized library of RBSs using the Salis Lab RBS generator. Then, plasmids with desired RBS strengths were screened for and selected. Refer to our protocol page for details in procedure.
