Difference between revisions of "Team:Dalhousie/Results"

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</br>Results</br>
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</br><h1><font color= "#C1D35D">Results</font></h1></br>
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What should this page contain?
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-Clearly and objectively describe the results of your work.
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Over the course of the 2017 iGEM season, we have had some downs, but many more ups.  
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-Future plans for the project.
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<font color= "#C1D35D"><h3>Project Achievements<h3></font></br>
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-Considerations for replicating the experiments.
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<font color= "#C1D35D">Successes</font></br>
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-You should also describe what your results mean:
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Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
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<ul>
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Show data, but remember all measurement and characterization data must be on part pages in the Registry.
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<li>Developed a pipeline to identify, or "mine", the porcupine metagenomic sequencing to discover novel enzymes.</li>
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Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.
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<li>Identified 8? novel enzymes with variable percent identity.</li>
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<li>Synthesized 5 of those enzymes, and successfully cloned 4 of them into psB1AK3.</li>
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<li>Optimized our previous biobrick Endoglucanse(<a href="http://parts.igem.org/Part:BBa_K2160000" style="color: #C1D35D">BBa_K2160000</a>)by adding a C termincal HIS-tag and N terminal PelB sequence (<a href="https://2017.igem.org/Team:Dalhousie/Improve" style="color:#C1D35D">Improve</a>).</li>
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<li>Successfully completed a fluorophore cleavage assay from the Hallam lab.</li>
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<li>Isolated high molecular weight DNA from porcupine fecal samples.</li>
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<li>Obtained efficient ligation and digestion with pJC8 controls. </li>
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<li>Produced phage plaque with the phage packaging extract lamba DNA controls. </li>
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</ul>
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<font color= "#C1D35D">Successes</font></br>
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<font color= "#C1D35D">DNA Extraction</font></br>
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Project Achievements
 
Project Achievements

Revision as of 20:17, 31 October 2017

Results

Results


Over the course of the 2017 iGEM season, we have had some downs, but many more ups.

Project Achievements


Successes
  • Developed a pipeline to identify, or "mine", the porcupine metagenomic sequencing to discover novel enzymes.
  • Identified 8? novel enzymes with variable percent identity.
  • Synthesized 5 of those enzymes, and successfully cloned 4 of them into psB1AK3.
  • Optimized our previous biobrick Endoglucanse(BBa_K2160000)by adding a C termincal HIS-tag and N terminal PelB sequence (Improve).
  • Successfully completed a fluorophore cleavage assay from the Hallam lab.
  • Isolated high molecular weight DNA from porcupine fecal samples.
  • Obtained efficient ligation and digestion with pJC8 controls.
  • Produced phage plaque with the phage packaging extract lamba DNA controls.
Successes
DNA Extraction
Project Achievements You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer. A list of linked bullet points of the successful results during your project A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.