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− | </br>Results</br> | + | </br><h1><font color= "#C1D35D">Results</font></h1></br> |
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− | + | Over the course of the 2017 iGEM season, we have had some downs, but many more ups. | |
− | + | <font color= "#C1D35D"><h3>Project Achievements<h3></font></br> | |
− | + | <font color= "#C1D35D">Successes</font></br> | |
− | + | ||
− | + | <ul> | |
− | + | <li>Developed a pipeline to identify, or "mine", the porcupine metagenomic sequencing to discover novel enzymes.</li> | |
− | + | <li>Identified 8? novel enzymes with variable percent identity.</li> | |
+ | <li>Synthesized 5 of those enzymes, and successfully cloned 4 of them into psB1AK3.</li> | ||
+ | |||
+ | <li>Optimized our previous biobrick Endoglucanse(<a href="http://parts.igem.org/Part:BBa_K2160000" style="color: #C1D35D">BBa_K2160000</a>)by adding a C termincal HIS-tag and N terminal PelB sequence (<a href="https://2017.igem.org/Team:Dalhousie/Improve" style="color:#C1D35D">Improve</a>).</li> | ||
+ | <li>Successfully completed a fluorophore cleavage assay from the Hallam lab.</li> | ||
+ | <li>Isolated high molecular weight DNA from porcupine fecal samples.</li> | ||
+ | <li>Obtained efficient ligation and digestion with pJC8 controls. </li> | ||
+ | <li>Produced phage plaque with the phage packaging extract lamba DNA controls. </li> | ||
+ | </ul> | ||
+ | |||
+ | <font color= "#C1D35D">Successes</font></br> | ||
+ | |||
+ | <font color= "#C1D35D">DNA Extraction</font></br> | ||
+ | |||
Project Achievements | Project Achievements |
Revision as of 20:17, 31 October 2017
Results
Results
Over the course of the 2017 iGEM season, we have had some downs, but many more ups.Project Achievements
Successes- Developed a pipeline to identify, or "mine", the porcupine metagenomic sequencing to discover novel enzymes.
- Identified 8? novel enzymes with variable percent identity.
- Synthesized 5 of those enzymes, and successfully cloned 4 of them into psB1AK3.
- Optimized our previous biobrick Endoglucanse(BBa_K2160000)by adding a C termincal HIS-tag and N terminal PelB sequence (Improve).
- Successfully completed a fluorophore cleavage assay from the Hallam lab.
- Isolated high molecular weight DNA from porcupine fecal samples.
- Obtained efficient ligation and digestion with pJC8 controls.
- Produced phage plaque with the phage packaging extract lamba DNA controls.