Results

Over the course of the 2017 iGEM season, we have had some downs, but many more ups.

Project Achievements

Successes

• Developed a pipeline to identify, or "mine", the porcupine metagenomic sequencing to discover novel enzymes.
• Identified 8? novel enzymes with variable percent identity.
• Synthesized 5 of those enzymes, and successfully cloned 4 of them into psB1AK3.
• Optimized our previous biobrick Endoglucanse(BBa_K2160000)by adding a C termincal HIS-tag and N terminal PelB sequence (Improve).
• Successfully completed a fluorophore cleavage assay from the Hallam lab.
• Isolated high molecular weight DNA from porcupine fecal samples.
• Obtained efficient ligation and digestion with pJC8 controls.
• Produced phage plaque with the phage packaging extract lamba DNA controls.

Successes
DNA Extraction
Project Achievements You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer. A list of linked bullet points of the successful results during your project A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.