Difference between revisions of "Team:ETH Zurich/Protocols"
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| − | <summary>Cultivation of | + | <summary>Cultivation of Mamalian Cell Lines </summary> |
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<a href="https://static.igem.org/mediawiki/2017/d/dd/T--ETH_Zurich--cultivation_of_mamalian_cells.pdf" download>Download</a> | <a href="https://static.igem.org/mediawiki/2017/d/dd/T--ETH_Zurich--cultivation_of_mamalian_cells.pdf" download>Download</a> | ||
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| − | <summary>Calcein AM | + | <summary>Calcein AM Staining Assay </summary> |
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<a href="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" download>Download</a> | <a href="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" download>Download</a> | ||
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<summary>Defining the Seeding Cell Concentration</summary> | <summary>Defining the Seeding Cell Concentration</summary> | ||
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| − | <a href="https://static.igem.org/mediawiki/2017/ | + | <a href="https://static.igem.org/mediawiki/2017/7/73/T--ETH_Zurich--seeding_experiment.pdf" download>Download</a> |
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Revision as of 20:42, 31 October 2017
Protocols
General Protocols
Chemical Transformation
DownloadColony PCR
DownloadConsumables
DownloadCryostock of Bacteria
DownloadElectroporation
DownloadGel Extraction
DownloadGibson Assembly
DownloadLigase Chain Reaction
DownloadMiniprep
DownloadPCR Clean-Up
DownloadPhusion PCR
DownloadPreparation of Chemically Competent Cells
DownloadPreparation of Electrocompetent Cells
DownloadRestriction Ligation
DownloadTumor Sensor
MRI Contrast Agent
Anti-Cancer Toxin
Fluorescence Measurement to Obtain the AHL Dose-Response Curve
DownloadSDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28
DownloadBacterial Lysate Production
DownloadCultivation of Mamalian Cell Lines
DownloadCalcein AM Staining Assay
DownloadKilling Curve of Different Cells Lines with 5-Fluouracil
DownloadDefining the Seeding Cell Concentration
DownloadLysate Tolerance of Different Cell Lines
DownloadKilling Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate
DownloadHeat Sensor
Heat-Induction of TlpA-controlled GFP Expression
DownloadCell Lysis
Heat-Induction of GFP Release by TlpA-controlled Protein E Expression
Download
