Difference between revisions of "Team:TU-Eindhoven/Team/Notebook"
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<br/><br/><br/><br/><br/><br/><br/><br/> | <br/><br/><br/><br/><br/><br/><br/><br/> | ||
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| + | <h1>Notebook</h1> | ||
| + | <h6>Throughout the past few months we have tracked our progress in our notebook. Below we give a short overview per month including what we have accomplished that month and what kind of experiments we did. For a more detailed overview of the labwork, click on the arrow.</h6><br/><br/> | ||
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| + | <h2>March - Building the Team</h2> | ||
| + | <h6>During this first month we divided the team roles and we started to brainstorm about project ideas and read a lot of literature for inspiration. Every week we had a meeting to discuss our progress and exchange ideas.</h6><br/><br/> | ||
| + | |||
| + | <h2>April - Constructing the Constructs</h2> | ||
| + | <h6>During this first month we divided the team roles and we started to brainstorm about project ideas and read a lot of literature for inspiration. Every week we had a meeting to discuss our progress and exchange ideas. | ||
| + | |||
| + | After a lot of brainstorm sessions we started to design our constructs and had meetings with our supervisors to finetune the idea. During the end of the month we started working on designing the DNA of the constructs. We also had a first session for our Human Practices as a preparation for SYNENERGENE at the end of the month.<br/><br/> | ||
| + | The first round of gBlocks were designed in such a way that they can be used with the gibson assembly method. We used the cloning guide from iGEM team Eindhoven 2015 as a starting point. For detailed information on our protein design click here.</h6> | ||
| + | <label class="collapse" for="_1"><img src="https://static.igem.org/mediawiki/2017/4/48/T--TU-Eindhoven--Arrow_Down.png" width="20" height="20" /></label> | ||
| + | <input id="_1" type="checkbox"> | ||
| + | <div><br /><h3>Week 1 - 03/04 - 07/04</h3><br/> | ||
| + | <h6>The first draft of our idea was made and the two constructs were designed:<br/> | ||
| + | a 14-3-3 tetramer with a n-term GFP and c-term ExoS peptide & CT33 fused to a strep-tag and mCherry.</h6><br/><br/> | ||
| + | |||
Revision as of 21:23, 31 October 2017
Notebook
Throughout the past few months we have tracked our progress in our notebook. Below we give a short overview per month including what we have accomplished that month and what kind of experiments we did. For a more detailed overview of the labwork, click on the arrow.
March - Building the Team
During this first month we divided the team roles and we started to brainstorm about project ideas and read a lot of literature for inspiration. Every week we had a meeting to discuss our progress and exchange ideas.
April - Constructing the Constructs
During this first month we divided the team roles and we started to brainstorm about project ideas and read a lot of literature for inspiration. Every week we had a meeting to discuss our progress and exchange ideas.
After a lot of brainstorm sessions we started to design our constructs and had meetings with our supervisors to finetune the idea. During the end of the month we started working on designing the DNA of the constructs. We also had a first session for our Human Practices as a preparation for SYNENERGENE at the end of the month.
The first round of gBlocks were designed in such a way that they can be used with the gibson assembly method. We used the cloning guide from iGEM team Eindhoven 2015 as a starting point. For detailed information on our protein design click here.
Week 1 - 03/04 - 07/04
The first draft of our idea was made and the two constructs were designed:
a 14-3-3 tetramer with a n-term GFP and c-term ExoS peptide & CT33 fused to a strep-tag and mCherry.
