Cas9 & Cpf1 secretion
and activity
Difference between revisions of "Template:Team:Utrecht/MainBody"
| Line 764: | Line 764: | ||
<br /> | <br /> | ||
<br /> | <br /> | ||
| + | <video style="width: 100%;" poster="" controls> | ||
| + | <source src="" type='video/mp4'/> | ||
| + | <source src="" type='video/ogg; codecs="theora, vorbis"'/> | ||
| + | <source src="" type='video/webm; codecs="vp8, vorbis"'/> | ||
| + | <a href=""><img border="0" src="" alt="Click to view on Youtube" width="100%"></a> | ||
| + | <p style="font-style:italic;color:red;border-style:solid;border-width:2px;border-color:red">Your browser either does not support HTML5 or cannot handle MediaWiki open video formats. Please consider upgrading your browser, installing the appropriate plugin or switching to a Firefox or Chrome install.</p> | ||
| + | </video> | ||
| + | |||
| + | <br><br> | ||
| + | |||
<h2 class="subhead" id="subhead-2">The problem</h2> | <h2 class="subhead" id="subhead-2">The problem</h2> | ||
Disease diagnosis is of great importance for healthcare. | Disease diagnosis is of great importance for healthcare. | ||
| Line 1,445: | Line 1,455: | ||
<script id="page-stakeholders" type="text/template"> | <script id="page-stakeholders" type="text/template"> | ||
| − | + | As OUTCASST is a system with a very broad application, we wanted to find the most suitable end users and focus our design on their needs and restrictions. To achieve this goal, we have interviewed multiple professionals from different backgrounds. In these conversations, we tried to discover strengths and weaknesses of our system and design with regard to existing techniques and whether the OUTCASST system would be useful in the professions of the interviewed individuals. If not, we tried to figure out in what direction it might prove more useful or what aspects needed to be improved. In addition, we also discussed safety issues and technical aspects of our toolkit design. | |
| − | + | <br><br> | |
| − | + | In the short video below, we show a summary of the journey we made to get to our end users. Besides this summary, more elaborate descriptions of each interview can be found below. | |
| − | + | <br><br> | |
| − | < | + | <video style="width: 100%;" poster="" controls> |
| + | <source src="" type='video/mp4'/> | ||
| + | <source src="" type='video/ogg; codecs="theora, vorbis"'/> | ||
| + | <source src="" type='video/webm; codecs="vp8, vorbis"'/> | ||
| + | <a href=""><img border="0" src="" alt="Click to view on Youtube" width="100%"></a> | ||
| + | <p style="font-style:italic;color:red;border-style:solid;border-width:2px;border-color:red">Your browser either does not support HTML5 or cannot handle MediaWiki open video formats. Please consider upgrading your browser, installing the appropriate plugin or switching to a Firefox or Chrome install.</p> | ||
| + | </video> | ||
<div class="shuffle-container" style="position: relative; margin-top: 30px; margin-bottom: 50px; width: 900px; margin-left: -75px;"> | <div class="shuffle-container" style="position: relative; margin-top: 30px; margin-bottom: 50px; width: 900px; margin-left: -75px;"> | ||
<div style="position: absolute; top: 200px; left: 350px; z-index: 1;"> | <div style="position: absolute; top: 200px; left: 350px; z-index: 1;"> | ||
| Line 2,566: | Line 2,582: | ||
<div class="team_members"> | <div class="team_members"> | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
| − | <div class="timeline-content timeline-card js--fadeInLeft"> | + | <div class="timeline-content timeline-card js--fadeInLeft"><div class="card"><div class="front"> |
<div class="timeline-img-header"> | <div class="timeline-img-header"> | ||
| − | <img width="100%" style="margin-top: - | + | <img width="100%" style="margin-top: -75px;" src=""> |
<h2>Niels Geijsen</h2> | <h2>Niels Geijsen</h2> | ||
</div> | </div> | ||
| − | < | + | <span style="display: block; font-size: 12px; padding: 0 20px;"> |
| − | <b>Background</b> | + | <b>Background</b> TODO<br> |
| − | < | + | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> |
| − | < | + | <span class="team-duties"> |
| − | </ | + | › a |
| + | </span> | ||
| + | |||
| + | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| + | <div class="team-bio" style="height: 61px;"> | ||
| + | Bio | ||
| + | <div class="team-bio-grad"></div> | ||
| + | </div> | ||
| + | <div class="team-expand">…</div> | ||
| + | <br> | ||
| + | </span> | ||
| + | </div> | ||
| + | <div class="back"></div></div> | ||
</div> | </div> | ||
| − | <div class="timeline-content timeline-card js--fadeInLeft"> | + | <div class="timeline-content timeline-card js--fadeInLeft"><div class="card"><div class="front"> |
<div class="timeline-img-header"> | <div class="timeline-img-header"> | ||
| − | <img width="100%" style="margin-top: - | + | <img width="100%" style="margin-top: -75px;" src=""> |
<h2>Roos Masereeuw</h2> | <h2>Roos Masereeuw</h2> | ||
</div> | </div> | ||
| − | < | + | <span style="display: block; font-size: 12px; padding: 0 20px;"> |
| − | <b>Background</b> | + | <b>Background</b> Professor of Experimental Pharmacology<br>Department of Pharmaceutical Sciences<br> |
| − | < | + | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> |
| − | < | + | <span class="team-duties"> |
| − | </ | + | › a |
| + | </span> | ||
| + | |||
| + | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| + | <div class="team-bio" style="height: 61px;"> | ||
| + | Bio | ||
| + | <div class="team-bio-grad"></div> | ||
| + | </div> | ||
| + | <div class="team-expand">…</div> | ||
| + | <br> | ||
| + | </span> | ||
| + | </div> | ||
| + | <div class="back"></div></div> | ||
</div> | </div> | ||
| − | <div class="timeline-content timeline-card js--fadeInLeft"> | + | <div class="timeline-content timeline-card js--fadeInLeft"><div class="card"><div class="front"> |
<div class="timeline-img-header"> | <div class="timeline-img-header"> | ||
| − | <img width="100%" style="margin-top: - | + | <img width="100%" style="margin-top: -75px;" src=""> |
<h2>Guido van den Ackerveken</h2> | <h2>Guido van den Ackerveken</h2> | ||
</div> | </div> | ||
| − | < | + | <span style="display: block; font-size: 12px; padding: 0 20px;"> |
| − | <b>Background</b> & | + | <b>Background</b> Professor of Translational Plant & Microbial Biology, Dept. Biology, Utrecht University<br> |
| − | < | + | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> |
| − | < | + | <span class="team-duties"> |
| − | </ | + | › a |
| + | </span> | ||
| + | |||
| + | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| + | <div class="team-bio" style="height: 61px;"> | ||
| + | Bio | ||
| + | <div class="team-bio-grad"></div> | ||
| + | </div> | ||
| + | <div class="team-expand">…</div> | ||
| + | <br> | ||
| + | </span> | ||
| + | </div> | ||
| + | <div class="back"></div></div> | ||
</div> | </div> | ||
| − | <div class="timeline-content timeline-card js--fadeInLeft"> | + | <div class="timeline-content timeline-card js--fadeInLeft"><div class="card"><div class="front"> |
| + | <div class="timeline-img-header"> | ||
| + | <img width="100%" style="margin-top: -75px;" src=""> | ||
| + | <h2>Margot Koster</h2> | ||
| + | </div> | ||
| + | <span style="display: block; font-size: 12px; padding: 0 20px;"> | ||
| + | <b>Background</b> Assistant professor of Microbiology<br> | ||
| + | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> | ||
| + | <span class="team-duties"> | ||
| + | › a | ||
| + | </span> | ||
| + | |||
| + | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| + | <div class="team-bio" style="height: 61px;"> | ||
| + | Bio | ||
| + | <div class="team-bio-grad"></div> | ||
| + | </div> | ||
| + | <div class="team-expand">…</div> | ||
| + | <br> | ||
| + | </span> | ||
| + | </div> | ||
| + | <div class="back"></div></div> | ||
| + | </div> | ||
| + | |||
| + | <div class="timeline-content timeline-card js--fadeInLeft"><div class="card"><div class="front"> | ||
<div class="timeline-img-header"> | <div class="timeline-img-header"> | ||
| − | <img width="100%" style="margin-top: - | + | <img width="100%" style="margin-top: -75px;" src=""> |
<h2>Clara Martinez Mir</h2> | <h2>Clara Martinez Mir</h2> | ||
</div> | </div> | ||
| − | < | + | <span style="display: block; font-size: 12px; padding: 0 20px;"> |
| − | <b>Background</b> | + | <b>Background</b> Cancer, Stem Cells and Developmental Biology<br> |
| − | < | + | <div style="display: inline-block; font-weight: bold; padding-top: 10px;">Responsibilities</div><br> |
| − | < | + | <span class="team-duties"> |
| − | </ | + | › Experimental - Secreting<br> Cas9 and Cpf1/MESA replication/Assembly |
| + | </span> | ||
| + | |||
| + | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
| + | <div class="team-bio" style="height: 61px;"> | ||
| + | Hello, my name is Clara (23) and I’m a first year Cancer, Stem Cells and Developmental biology Master student in Utrecht. I finished a Bachelor’s degree in Genetics in Barcelona last year and came to the Netherlands with a fellowship for post-graduate internships abroad, which allowed me to join Niels Geijsen’s lab for six months. | ||
| + | <br><br> | ||
| + | I’m really happy to have been asked to write a piece as part of the team, even if I haven’t been there for the whole process. I joined the team during the summer period to help with some lab work that was being carried out in my former lab. It has been a great experience to help supervising the Utrecht iGEM team and its amazing people, full of joy and stress I have to admit. Now the deadline is getting closer and we are all preparing for the big final! Thank you, guys, for this opportunity! | ||
| + | <div class="team-bio-grad"></div> | ||
| + | </div> | ||
| + | <div class="team-expand">…</div> | ||
| + | <br> | ||
| + | </span> | ||
| + | </div> | ||
| + | <div class="back"></div></div> | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 22:07, 31 October 2017
<!DOCTYPE html>
Comparison of endonuclease activity for Cas9 and Cpf1 that has been produced in, and excreted by, HEK293 cells.
MESA two-component system replication
Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.
OUTCASST system production
Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.
Modeling and
mathematics
mathematics
Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.
InterLab study participation
Results and details of our measurements for the iGEM 2017 InterLab Study.
Stakeholders & opinions
Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.
Risks & safety-issues
Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.
Design & integration
OUTCASST toolkit and product design with factors such as bio-safety and user-friendliness taken into account.
Outreach
Videos we made for the dutch public, together with 'de Kennis van Nu'.
Meet our team
About us, our interests and roles in the team and our supervisors.
Sponsors
A listing of our sponsors, how they assisted us and our gratitude for their assistance.
Collaborations
Read about our exchanges with other iGEM teams and government agencies.
Achievements
A short description of all that we have achieved during our participation in the iGEM.
Attributions
A thank-you for everyone that assited us, both in and outside the lab.
×
