Difference between revisions of "Team:WLC-Milwaukee/Description"
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<h1>Description</h1> | <h1>Description</h1> | ||
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<h2>Step one</h2> | <h2>Step one</h2> | ||
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<h2>Step three</h2> | <h2>Step three</h2> | ||
<p>Substrate is added to the filter which will be cleaved by the enzyme. Color change occurs if <i>E. coli</i> is present. Note, each <i>E. coli</i> cell can bind hundreds of phage tail and each tail may bind several enzyme, which allows for high sensitivity. </p> | <p>Substrate is added to the filter which will be cleaved by the enzyme. Color change occurs if <i>E. coli</i> is present. Note, each <i>E. coli</i> cell can bind hundreds of phage tail and each tail may bind several enzyme, which allows for high sensitivity. </p> | ||
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| + | <img src="https://static.igem.org/mediawiki/2017/6/6d/T--WLC-Milwaukee--Filter_Paper.gif> | ||
Revision as of 22:45, 31 October 2017
Description
Step two
The solution is syringe filtered through a 0.45 micrometer filter to trap bacteria bound to phage tails and allow any unbound phage tail through. This prevents false positives by ridding the solution of excess phage tail for step three.
Step three
Substrate is added to the filter which will be cleaved by the enzyme. Color change occurs if E. coli is present. Note, each E. coli cell can bind hundreds of phage tail and each tail may bind several enzyme, which allows for high sensitivity.
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