Difference between revisions of "Team:ETH Zurich/Protocols"

 
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<main role="main">
 
<main role="main">
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<h1 class="headline">Protocols</h1>
  
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<section class="first">
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<h1>General Protocols</h1>
  
 +
<div class="multi-summary protocols">
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<details>
 +
<summary>Chemical Transformation</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" download>Download</a>
 +
</details>
  
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<h1 class="headline">Protocols</h1>
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<details>
 +
<summary>Colony PCR</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" download>Download</a>
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</details>
  
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<section>
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<details>
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<h1>General Protocols</h1>
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<summary>Consumables</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" download>Download</a>
 +
</details>
  
 
<details>
 
<details>
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        <summary>Chemical Transformation</summary>
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<summary>Cryostock of Bacteria</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Colony PCR</summary>
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<summary>Electroporation</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Consumables</summary>
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<summary>Gel Extraction</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Cryostock of Bacteria</summary>
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<summary>Gibson Assembly</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Electroporation</summary>
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<summary>Ligase Chain Reaction</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Gel Extraction</summary>
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<summary>Miniprep</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>Gibson Assembly</summary>
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<summary>PCR Clean-Up</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Ligase Chain Reaction</summary>
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<summary>Phusion PCR</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Miniprep</summary>
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<summary>Preparation of Chemically Competent Cells</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>PCR Clean-Up</summary>
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<summary>Preparation of Electrocompetent Cells</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>Phusion PCR</summary>
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<summary>Restriction Ligation</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download>Download</a>
 
</details>
 
</details>
 +
</div>
 +
</section>
  
 +
<section>
 +
<h1>Tumor Sensor</h1>
 +
<div class="multi-summary protocols">
 
<details>
 
<details>
−
        <summary>Preparation of Chemically Competent Cells</summary>
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<summary>Quorum Sensing End-Point Characterization</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/4/49/T--ETH_Zurich--Quorum_Sensing_End_Point_Characterization.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/4/49/T--ETH_Zurich--Quorum_Sensing_End_Point_Characterization.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>Preparation of Electrocompetent Cells</summary>
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<summary>AND-gate Without Quorum Sensing</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/f/f5/T--ETH_Zurich--AND-gate_without_Quorum_Sensing.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/f/f5/T--ETH_Zurich--AND-gate_without_Quorum_Sensing.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>Restriction Ligation</summary>
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<summary>AND-gate With Quorum Sensing</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/d/d2/T--ETH_Zurich--AND-gate_with_Quorum_Sensing.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/d/d2/T--ETH_Zurich--AND-gate_with_Quorum_Sensing.pdf" download>Download</a>
 
</details>
 
</details>
 +
</div>
 +
</section>
  
 +
<section>
 +
<h1>MRI Contrast Agent</h1>
 +
<div class="multi-summary protocols">
 +
<details>
 +
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 +
</details>
 +
 +
<details>
 +
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 +
</details>
 +
 +
<details>
 +
<summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <span class="bacterium">E. coli</span> Nissle</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" download>Download</a>
 +
</details>
 +
</div>
 
</section>
 
</section>
  
 +
<section>
 +
<h1>Anti-Cancer Toxin</h1>
 +
<div class="multi-summary protocols">
 +
<details>
 +
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 +
</details>
  
 +
<details>
 +
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 +
</details>
  
−
<!--
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<details>
−
<section class="first">
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<summary>Bacterial Lysate Production</summary>
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    <h1>What should this page contain?</h1>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/f/fb/T--ETH_Zurich--bacterial_lysate.pdf" width="800"/>
−
    <p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments. Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.</p>
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<a href="https://static.igem.org/mediawiki/2017/f/fb/T--ETH_Zurich--bacterial_lysate.pdf" download>Download</a>
−
    <ul>
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</details>
−
        <li>Protocols</li>
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−
        <li>Experiments</li>
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<details>
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        <li>Documentation of the development of your project</li>
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<summary>Cultivation of Mamalian Cell Lines </summary>
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    </ul>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/d/dd/T--ETH_Zurich--cultivation_of_mamalian_cells.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/d/dd/T--ETH_Zurich--cultivation_of_mamalian_cells.pdf" download>Download</a>
 +
</details>
 +
 
 +
<details>
 +
<summary>Calcein AM Staining Assay </summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" download>Download</a>
 +
</details>
 +
 
 +
<details>
 +
<summary>Killing Curve of Different Cells Lines with 5-Fluouracil</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/2a/T--ETH_Zurich--fluouracil_killing_curve.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/2/2a/T--ETH_Zurich--fluouracil_killing_curve.pdf" download>Download</a>
 +
</details>
 +
 
 +
<details>
 +
<summary>Defining the Seeding Cell Concentration</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/7/73/T--ETH_Zurich--seeding_experiment.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/7/73/T--ETH_Zurich--seeding_experiment.pdf" download>Download</a>
 +
</details>
 +
 
 +
<details>
 +
<summary>Lysate Tolerance of Different Cell Lines</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/7/78/T--ETH_Zurich--lysate_tolerance.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/7/78/T--ETH_Zurich--lysate_tolerance.pdf" download>Download</a>
 +
</details>
 +
 
 +
 
 +
<details>
 +
<summary>Killing Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/b/b3/T--ETH_Zurich--azurin_killing_curve.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/b/b3/T--ETH_Zurich--azurin_killing_curve.pdf" download>Download</a>
 +
</details>
 +
 
 +
 
 +
 
 +
 
 +
</div>
 
</section>
 
</section>
 +
 
<section>
 
<section>
−
    <h1>Inspiration</h1>
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<h1>Heat Sensor</h1>
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    <ul>
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<div class="multi-summary protocols">
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        <li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia</a></li>
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<details>
−
        <li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial</a></li>
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<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
−
        <li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech</a></li>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" width="800"/>
−
    </ul>
+
<a href="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" download>Download</a>
 +
</details>
 +
</div>
 
</section>
 
</section>
−
-->
+
 
 +
<section>
 +
<h1>Cell Lysis</h1>
 +
<div class="multi-summary protocols">
 +
<details>
 +
<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" download>Download</a>
 +
</details>
 +
</div>
 +
</section>
 +
 
 
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Latest revision as of 22:52, 31 October 2017

Protocols

General Protocols

Chemical Transformation Download
Colony PCR Download
Consumables Download
Cryostock of Bacteria Download
Electroporation Download
Gel Extraction Download
Gibson Assembly Download
Ligase Chain Reaction Download
Miniprep Download
PCR Clean-Up Download
Phusion PCR Download
Preparation of Chemically Competent Cells Download
Preparation of Electrocompetent Cells Download
Restriction Ligation Download

Tumor Sensor

Quorum Sensing End-Point Characterization Download
AND-gate Without Quorum Sensing Download
AND-gate With Quorum Sensing Download

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin Download
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle Download

Anti-Cancer Toxin

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28 Download
Bacterial Lysate Production Download
Cultivation of Mamalian Cell Lines Download
Calcein AM Staining Assay Download
Killing Curve of Different Cells Lines with 5-Fluouracil Download
Defining the Seeding Cell Concentration Download
Lysate Tolerance of Different Cell Lines Download
Killing Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate Download

Heat Sensor

Heat-Induction of TlpA-controlled GFP Expression Download

Cell Lysis

Heat-Induction of GFP Release by TlpA-controlled Protein E Expression Download