Difference between revisions of "Team:ETH Zurich/Protocols"

 
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<h1 class="headline">Protocols</h1>
 
<h1 class="headline">Protocols</h1>
  
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<section>
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<section class="first">
 
<h1>General Protocols</h1>
 
<h1>General Protocols</h1>
  
 +
<div class="multi-summary protocols">
 
<details>
 
<details>
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        <summary>Chemical Transformation</summary>
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<summary>Chemical Transformation</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Colony PCR</summary>
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<summary>Colony PCR</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Consumables</summary>
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<summary>Consumables</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Cryostock of Bacteria</summary>
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<summary>Cryostock of Bacteria</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Electroporation</summary>
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<summary>Electroporation</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Gel Extraction</summary>
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<summary>Gel Extraction</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Gibson Assembly</summary>
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<summary>Gibson Assembly</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Ligase Chain Reaction</summary>
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<summary>Ligase Chain Reaction</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>Miniprep</summary>
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<summary>Miniprep</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>PCR Clean-Up</summary>
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<summary>PCR Clean-Up</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Phusion PCR</summary>
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<summary>Phusion PCR</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Preparation of Chemically Competent Cells</summary>
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<summary>Preparation of Chemically Competent Cells</summary>
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        <embed src="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
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        <summary>Preparation of Electrocompetent Cells</summary>
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<summary>Preparation of Electrocompetent Cells</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>Restriction Ligation</summary>
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<summary>Restriction Ligation</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" width="100%" height="700px"/>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download>Download</a>
 
</details>
 
</details>
 +
</div>
 
</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>Tumor Sensor</h1>
 
<h1>Tumor Sensor</h1>
 +
<div class="multi-summary protocols">
 
<details>
 
<details>
−
        <summary>Quorum Sensing End-Point Characterization</summary>
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<summary>Quorum Sensing End-Point Characterization</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/4/49/T--ETH_Zurich--Quorum_Sensing_End_Point_Characterization.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/4/49/T--ETH_Zurich--Quorum_Sensing_End_Point_Characterization.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>AND-gate Without Quorum Sensing</summary>
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<summary>AND-gate Without Quorum Sensing</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/f/f5/T--ETH_Zurich--AND-gate_without_Quorum_Sensing.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/f/f5/T--ETH_Zurich--AND-gate_without_Quorum_Sensing.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>AND-gate With Quorum Sensing</summary>
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<summary>AND-gate With Quorum Sensing</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/d/d2/T--ETH_Zurich--AND-gate_with_Quorum_Sensing.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/d/d2/T--ETH_Zurich--AND-gate_with_Quorum_Sensing.pdf" download>Download</a>
 
</details>
 
</details>
 +
</div>
 
</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>MRI Contrast Agent</h1>
 
<h1>MRI Contrast Agent</h1>
 +
<div class="multi-summary protocols">
 
<details>
 
<details>
−
        <summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
+
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary>
+
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <i>E. coli</i> Nissle</summary>
+
<summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <span class="bacterium">E. coli</span> Nissle</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" download>Download</a>
 
</details>
 
</details>
−
 
+
</div>
 
</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>Anti-Cancer Toxin</h1>
 
<h1>Anti-Cancer Toxin</h1>
 +
<div class="multi-summary protocols">
 
<details>
 
<details>
−
        <summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
+
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
        <summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary>
+
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 
</details>
 
</details>
 +
 +
<details>
 +
<summary>Bacterial Lysate Production</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/f/fb/T--ETH_Zurich--bacterial_lysate.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/f/fb/T--ETH_Zurich--bacterial_lysate.pdf" download>Download</a>
 +
</details>
 +
 +
<details>
 +
<summary>Cultivation of Mamalian Cell Lines </summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/d/dd/T--ETH_Zurich--cultivation_of_mamalian_cells.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/d/dd/T--ETH_Zurich--cultivation_of_mamalian_cells.pdf" download>Download</a>
 +
</details>
 +
 +
<details>
 +
<summary>Calcein AM Staining Assay </summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" download>Download</a>
 +
</details>
 +
 +
<details>
 +
<summary>Killing Curve of Different Cells Lines with 5-Fluouracil</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/2a/T--ETH_Zurich--fluouracil_killing_curve.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/2/2a/T--ETH_Zurich--fluouracil_killing_curve.pdf" download>Download</a>
 +
</details>
 +
 +
<details>
 +
<summary>Defining the Seeding Cell Concentration</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/7/73/T--ETH_Zurich--seeding_experiment.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/7/73/T--ETH_Zurich--seeding_experiment.pdf" download>Download</a>
 +
</details>
 +
 +
<details>
 +
<summary>Lysate Tolerance of Different Cell Lines</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/7/78/T--ETH_Zurich--lysate_tolerance.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/7/78/T--ETH_Zurich--lysate_tolerance.pdf" download>Download</a>
 +
</details>
 +
 +
 +
<details>
 +
<summary>Killing Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/b/b3/T--ETH_Zurich--azurin_killing_curve.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/b/b3/T--ETH_Zurich--azurin_killing_curve.pdf" download>Download</a>
 +
</details>
 +
 +
 +
 +
 +
</div>
 
</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>Heat Sensor</h1>
 
<h1>Heat Sensor</h1>
 +
<div class="multi-summary protocols">
 
<details>
 
<details>
−
        <summary>Heat-Induction of TlpA-Repressed GFP Expression</summary>
+
<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
−
        <embed src="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" width="100%" height="700px"/>
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" download>Download</a>
 
</details>
 
</details>
 +
</div>
 
</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>Cell Lysis</h1>
 
<h1>Cell Lysis</h1>
 +
<div class="multi-summary protocols">
 +
<details>
 +
<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" width="800"/>
 +
<a href="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" download>Download</a>
 +
</details>
 +
</div>
 
</section>
 
</section>
  
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    <h1>What should this page contain?</h1>
 
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    <p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments. Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.</p>
 
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        <li>Protocols</li>
 
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    <h1>Inspiration</h1>
 
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        <li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia</a></li>
 
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        <li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial</a></li>
 
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        <li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech</a></li>
 
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Latest revision as of 22:52, 31 October 2017

Protocols

General Protocols

Chemical Transformation Download
Colony PCR Download
Consumables Download
Cryostock of Bacteria Download
Electroporation Download
Gel Extraction Download
Gibson Assembly Download
Ligase Chain Reaction Download
Miniprep Download
PCR Clean-Up Download
Phusion PCR Download
Preparation of Chemically Competent Cells Download
Preparation of Electrocompetent Cells Download
Restriction Ligation Download

Tumor Sensor

Quorum Sensing End-Point Characterization Download
AND-gate Without Quorum Sensing Download
AND-gate With Quorum Sensing Download

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin Download
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle Download

Anti-Cancer Toxin

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28 Download
Bacterial Lysate Production Download
Cultivation of Mamalian Cell Lines Download
Calcein AM Staining Assay Download
Killing Curve of Different Cells Lines with 5-Fluouracil Download
Defining the Seeding Cell Concentration Download
Lysate Tolerance of Different Cell Lines Download
Killing Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate Download

Heat Sensor

Heat-Induction of TlpA-controlled GFP Expression Download

Cell Lysis

Heat-Induction of GFP Release by TlpA-controlled Protein E Expression Download