Difference between revisions of "Team:ETH Zurich/Protocols"

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<h1>General Protocols</h1>
 
<h1>General Protocols</h1>
  
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<details>
 
<summary>Chemical Transformation</summary>
 
<summary>Chemical Transformation</summary>
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<a download="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf">Download</a>
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<a href="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
 
<summary>Colony PCR</summary>
 
<summary>Colony PCR</summary>
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<a href="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Consumables</summary>
 
<summary>Consumables</summary>
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<a href="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Cryostock of Bacteria</summary>
 
<summary>Cryostock of Bacteria</summary>
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<a href="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Electroporation</summary>
 
<summary>Electroporation</summary>
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<a href="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Gel Extraction</summary>
 
<summary>Gel Extraction</summary>
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<a href="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Gibson Assembly</summary>
 
<summary>Gibson Assembly</summary>
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<a href="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Ligase Chain Reaction</summary>
 
<summary>Ligase Chain Reaction</summary>
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<a href="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Miniprep</summary>
 
<summary>Miniprep</summary>
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<a href="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>PCR Clean-Up</summary>
 
<summary>PCR Clean-Up</summary>
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<a href="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Phusion PCR</summary>
 
<summary>Phusion PCR</summary>
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<a href="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Preparation of Chemically Competent Cells</summary>
 
<summary>Preparation of Chemically Competent Cells</summary>
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<details>
 
<details>
 
<summary>Preparation of Electrocompetent Cells</summary>
 
<summary>Preparation of Electrocompetent Cells</summary>
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<details>
 
<details>
 
<summary>Restriction Ligation</summary>
 
<summary>Restriction Ligation</summary>
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<a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download>Download</a>
 
</details>
 
</details>
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</div>
 
</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>Tumor Sensor</h1>
 
<h1>Tumor Sensor</h1>
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<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Quorum Sensing End-Point Characterization</summary>
 
<summary>Quorum Sensing End-Point Characterization</summary>
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<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
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<a href="https://static.igem.org/mediawiki/2017/4/49/T--ETH_Zurich--Quorum_Sensing_End_Point_Characterization.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
 
<summary>AND-gate Without Quorum Sensing</summary>
 
<summary>AND-gate Without Quorum Sensing</summary>
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<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
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<a href="https://static.igem.org/mediawiki/2017/f/f5/T--ETH_Zurich--AND-gate_without_Quorum_Sensing.pdf" download>Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
 
<summary>AND-gate With Quorum Sensing</summary>
 
<summary>AND-gate With Quorum Sensing</summary>
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<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
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<a href="https://static.igem.org/mediawiki/2017/d/d2/T--ETH_Zurich--AND-gate_with_Quorum_Sensing.pdf" download>Download</a>
 
</details>
 
</details>
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</div>
 
</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>MRI Contrast Agent</h1>
 
<h1>MRI Contrast Agent</h1>
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<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
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<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
</details>
 
</details>
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<details>
 
<details>
 
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary>
 
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin</summary>
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<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <span class="bacterium">E. coli</span> Nissle</summary>
 
<summary>Magnetic Resonance Imagine of Bacterioferritin-Expressing <span class="bacterium">E. coli</span> Nissle</summary>
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<a href="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/a/a8/T--ETH_Zurich--protocolfb3.pdf" download>Download</a>
 
</details>
 
</details>
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</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>Anti-Cancer Toxin</h1>
 
<h1>Anti-Cancer Toxin</h1>
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<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
 
<summary>Fluorescence Measurement to Obtain the AHL Dose-Response Curve</summary>
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<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/a/ac/T--ETH_Zurich--protocolfb1.pdf" download>Download</a>
 
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<details>
 
<details>
 
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary>
 
<summary>SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28</summary>
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<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/8/89/T--ETH_Zurich--protocolfb2.pdf" download>Download</a>
 
</details>
 
</details>
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<details>
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<summary>Bacterial Lysate Production</summary>
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<a href="https://static.igem.org/mediawiki/2017/f/fb/T--ETH_Zurich--bacterial_lysate.pdf" download>Download</a>
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</details>
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<details>
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<summary>Cultivation of Mamalian Cell Lines </summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/d/dd/T--ETH_Zurich--cultivation_of_mamalian_cells.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/d/dd/T--ETH_Zurich--cultivation_of_mamalian_cells.pdf" download>Download</a>
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</details>
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<details>
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<summary>Calcein AM Staining Assay </summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" download>Download</a>
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</details>
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<details>
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<summary>Killing Curve of Different Cells Lines with 5-Fluouracil</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/2a/T--ETH_Zurich--fluouracil_killing_curve.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/2/2a/T--ETH_Zurich--fluouracil_killing_curve.pdf" download>Download</a>
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</details>
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<details>
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<summary>Defining the Seeding Cell Concentration</summary>
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</details>
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<details>
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<summary>Lysate Tolerance of Different Cell Lines</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/7/78/T--ETH_Zurich--lysate_tolerance.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/7/78/T--ETH_Zurich--lysate_tolerance.pdf" download>Download</a>
 +
</details>
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<details>
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<summary>Killing Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/b/b3/T--ETH_Zurich--azurin_killing_curve.pdf" width="800"/>
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<a href="https://static.igem.org/mediawiki/2017/b/b3/T--ETH_Zurich--azurin_killing_curve.pdf" download>Download</a>
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</details>
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</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>Heat Sensor</h1>
 
<h1>Heat Sensor</h1>
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<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
 
<summary>Heat-Induction of TlpA-controlled GFP Expression</summary>
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<a href="https://static.igem.org/mediawiki/2017/9/96/T--ETH_Zurich--protocolfd1.pdf" download>Download</a>
 
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</details>
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</section>
 
</section>
  
 
<section>
 
<section>
 
<h1>Cell Lysis</h1>
 
<h1>Cell Lysis</h1>
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<div class="multi-summary protocols">
 
<details>
 
<details>
 
<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>
 
<summary>Heat-Induction of GFP Release by TlpA-controlled Protein E Expression</summary>
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<a href="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" download>Download</a>
 
<a href="https://static.igem.org/mediawiki/2017/e/e9/T-ETH_Zurich--Fe-protocol.pdf" download>Download</a>
 
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</section>
 
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Latest revision as of 22:52, 31 October 2017

Protocols

General Protocols

Chemical Transformation Download
Colony PCR Download
Consumables Download
Cryostock of Bacteria Download
Electroporation Download
Gel Extraction Download
Gibson Assembly Download
Ligase Chain Reaction Download
Miniprep Download
PCR Clean-Up Download
Phusion PCR Download
Preparation of Chemically Competent Cells Download
Preparation of Electrocompetent Cells Download
Restriction Ligation Download

Tumor Sensor

Quorum Sensing End-Point Characterization Download
AND-gate Without Quorum Sensing Download
AND-gate With Quorum Sensing Download

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin Download
Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle Download

Anti-Cancer Toxin

Fluorescence Measurement to Obtain the AHL Dose-Response Curve Download
SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28 Download
Bacterial Lysate Production Download
Cultivation of Mamalian Cell Lines Download
Calcein AM Staining Assay Download
Killing Curve of Different Cells Lines with 5-Fluouracil Download
Defining the Seeding Cell Concentration Download
Lysate Tolerance of Different Cell Lines Download
Killing Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate Download

Heat Sensor

Heat-Induction of TlpA-controlled GFP Expression Download

Cell Lysis

Heat-Induction of GFP Release by TlpA-controlled Protein E Expression Download