|
|
Line 143: |
Line 143: |
| care of to improve the efficiency of the reaction.</h3></section> | | care of to improve the efficiency of the reaction.</h3></section> |
| <br/> | | <br/> |
| + | <section><h3>It has been already documented that bacteria culture can be grown on paper even in a |
| + | cordinated manner with the help of inkjet paper [1]. To do this, paper is completely |
| + | immersed in toluene solution of polystyrene and then completely drying for making the |
| + | hydrophobic surface [1]. Then, small patches are made by inkjet paper with toluene to |
| + | form hydrophilic patches [1]. Agar is hydrolysed by sulphuric acid for appropriate viscosity |
| + | and dispensed with inkjet printer [1]. Sodium hydroxide can be used to neutralise the agar |
| + | and sodium sulphate can be removed by electrodialysis [1]. The agar is printed on the |
| + | hydrophilic patches and can be used to grow bacteria [1]. The paper is folded in a specific |
| + | manner to be accommodated inside the device as shown in the image.</h3> |
| + | </section> |
| + | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/0/0b/T--IISER-Mohali-INDIA--hardware1.png" width="100%"></td></tr></table></center> |
| + | |
| + | |
| + | |
| <section> | | <section> |
| <h3>References:</h3> | | <h3>References:</h3> |
| <h4> | | <h4> |
| + | 1. Srimongkon, T., Ishida, T., Igarashi, K., & Enomae, T. (2014). Development of a bacterial |
| + | culture system using a paper platform to accommodate media and an ink-jet printing to |
| + | dispense bacteria. Am. J. Biochem. Biotechnol, 10, 81-87. |
| + | </h4><h4> |
| Schmidt, A., Frensch, M., Schleheck, D., Schink, B., & Müller, N. (2014).Degradation of Acetaldehyde and Its Precursors by <i>Pelobactercarbinolicus</i> and <i>P. acetylenicus. PloS one</i>, 9(12), e115902.</h4> | | Schmidt, A., Frensch, M., Schleheck, D., Schink, B., & Müller, N. (2014).Degradation of Acetaldehyde and Its Precursors by <i>Pelobactercarbinolicus</i> and <i>P. acetylenicus. PloS one</i>, 9(12), e115902.</h4> |
| <h4> | | <h4> |