Difference between revisions of "Team:Dalhousie"

 
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      <li class="active" ><a href="https://2017.igem.org/Team:Dalhousie" style=" color: white;">Home</a></li>
  
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        <a class="dropdown-toggle" data-toggle="dropdown" href="#" style=" color: white;">Project
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          <li><a href="https://2017.igem.org/Team:Dalhousie/Description" >Description</a></li>
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          <li><a href="https://2017.igem.org/Team:Dalhousie/Design">Design</a></li>
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<li><a href="https://2017.igem.org/Team:Dalhousie/Requirements">Requirements</a></li>
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          <li><a href="https://2017.igem.org/Team:Dalhousie/InterLab">Interlab</a></li>
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          <li><a href="https://2017.igem.org/Team:Dalhousie/Team">Team</a></li>
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          <li><a href="https://2017.igem.org/Team:Dalhousie/Collaborations">Collaborations</a></li>
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          <li><a href="https://2017.igem.org/Team:Dalhousie/Attributions">Attributions</a></li>
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          <li><a href="https://2017.igem.org/Team:Dalhousie/HP/Silver">Science Communication</a></li>
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          <li><a href="https://2017.igem.org/Team:Dalhousie/HP/Gold_Integrated">Integrated and Gold</a></li>
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    <title>Dalhousie iGem</title>
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<div class="top" ><div class="title" >Dalhousie iGEM 2017</div></div>
  
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                        <a class="page-scroll" href="#about">The Project</a>
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                <h2>The Project</h2>
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Canada’s forests represent not only an established source of economic revenue, but also a potential source for biofuel substrate. Ligno-cellulosic by-products from pulp and paper processing are removed using chemicals, heat, and water. Microbial cellulose-degrading enzymes are high-value targets for industrial applications. Focused on innovative applications of synthetic biology, the undergraduate Dalhousie iGEM team has undertaken a multi-year project to harness the degradative capacity of microorganisms to convert cellulose into ethanol for biofuel applications. Advances in DNA sequencing technology and bioinformatics have revolutionized our ability to identify useful genes in complex biological samples. We hypothesize that the porcupine microbiome, which includes microorganisms capable of digesting bark and tree resin, will be a rich source of these useful genes. If our hypothesis is correct, mining the porcupine microbiome has the advantage of finding a suite of enzymes that have evolved to work in concert to efficiently degrade cellulose. </p>
 
 
<h4>The Project</h4>
 
<p style="text-align:center">
 
Using a combination of metagenomic library construction and bioinformatic analysis we aim to find known cellulolytic enzymes as well as discover novel enzymes.
 
Screening of our metagenomic library will allow us to look for a diverse selection of enzymes, including cellulolytic and lignolytic ones by plating on cellulose-only media. The clones that grow can be sequenced for confirmation and cloned into biobricks.
 
Our new bioinformatic pipeline now allows us to look for conserved domains rather than known enzymes. This is extremely powerful as it may allow us to discover new enzymes that share catalytic domains with cellulases but are otherwise totally novel.
 
The goal of this project is to construct a bioreactor where cellulose would be the input, D-glucose the output and E.coli expressing a suite of cellulolytic enzymes would be the workhorse, converting one to the other. Future goals would be to combine this in co-culture with yeast for the last step in bio-ethanol production.</p>
 
  
<h4>Human Practices </h4>
 
<p style="text-align:center">
 
As we progress through our project collaborating with industry, community and academia, we have noticed just how important science communication is. Selling our story has been important for getting funding, initiating community support and presenting to groups at Dalhousie University who aren’t versed in synthetic biology. As we reflected on how important science communication is, we broadened our scope to Halifax’s recent measles outbreaks and saw just how much pain science miscommunication can cause.
 
Our goal this year is to better understand the scope of scientific literacy within the general public through a survey asking ten short questions on how people read scientific media ( interested? Here’s the link). This information will allow us to draw some conclusions about people's reading and sharing habits online. This can then be used to interview science communicators, media outlets, scientists and members of the general public to get more fleshed out opinions on how science communication can improve, and how miscommunication can be spotted in the media. From this, we would like to create a webpage describing how to maneuver science communication and scientific media in the mainstream, with specific tips on how to spot fake articles. Our overarching goal is to discover ways science communication can progress to make science more accessible, and in the meantime create a document to help guide people through the maze of real science news and fake science news.
 
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<h1>
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<center><font color= "#ffffff">Mining the Microbiome</font></center>
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</h1>
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<center><img src="https://static.igem.org/mediawiki/parts/3/34/BIG_FRont_DESIGN.png" height="617" width="1097" style="margin-bottom:40px;"></center> 
 
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                    <h2>Contact us</h2>
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                    <p style="text-align:center">Feel free to contact us to collab, or to just say hello!</p>
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 +
width:100%;
 +
 
 +
}
 +
 
 +
#nav .inner {
 +
z-index:100;
 +
padding-bottom:40px;
 +
color: red;
 +
}
 +
 
 +
#nav ul {
 +
list-style: none;
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
 
 +
 
 +
#inner{
 +
z-index:101;
 +
 
 +
}
 +
 
 +
#panel1{
 +
background: transparent;
 +
height: 950px;
 +
width:100%;
 +
z-index:100;
 +
}
 +
#panel2{
 +
width:100%;
 +
height: 600px;
 +
margin-bottom:0px;
 +
background:  white;
 +
color: white;
 +
font-size: 40px;
 +
line-height: 40px;
 +
background-image: url("https://static.igem.org/mediawiki/2017/a/a5/Woodbackground.jpeg");
 +
height: 200%;
 +
}
 +
#panel3{
 +
width:100%;
 +
height: 100%;
 +
margin-bottom:-100px;
 +
background: rgba(35,47,19,0.9);
 +
}
 +
 
 +
 
 +
 
 +
 
 +
</style>
 +
<script>
 +
$( function() {
 +
//caches a jQuery object containing the header element
 +
var header = $('.noBackground');
 +
$(window).scroll(function() {
 +
var scroll = $(window).scrollTop();
 +
 
 +
if (scroll >= 100) {
 +
header.addClass('blackBackground');
 +
} else {
 +
header.removeClass('blackBackground');
 +
                     
 +
}
 +
});
 +
});
 +
</script>
 +
 
 +
</body>
 +
<html>

Latest revision as of 00:13, 1 November 2017

Dalhousie iGEM 2017

Mining the Microbiome