Difference between revisions of "Team:Shanghaitech/Experiment and Modeling"
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| + | <h2>Testing Fluorescent AHL Receivers</h2> | ||
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| + | <h2>Orthogonality test</h2> | ||
| + | Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct. | ||
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| + | [[File:T--Shanghaitech--LasRorthogonality.png|thumb|center|800px|<b>Fig. 2 Orthogonality test of LasR-pLas-GFP </b> (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL ]] | ||
Revision as of 00:44, 1 November 2017
Experiment and Modeling
Testing Fluorescent AHL Receivers
Orthogonality test
Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct. [[File:T--Shanghaitech--LasRorthogonality.png|thumb|center|800px|Fig. 2 Orthogonality test of LasR-pLas-GFP (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL ]]