Difference between revisions of "Team:Shanghaitech/Experiment and Modeling"

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<h2>Orthogonality test</h2>
 
<h2>Orthogonality test</h2>
 
Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct.
 
Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct.
<img src='File:T--Shanghaitech--LasRorthogonality.png' alt='<b>Fig. 2 Orthogonality test of LasR-pLas-GFP </b> (i):Fluorescent response to cognate and  non-cognate AHLs (ii)Dose-Response curves for cognate and  non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL' /></p>
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<img src='https://static.igem.org/mediawiki/parts/7/78/T--Shanghaitech--LasRorthogonality.png'alt='<b>Fig. 2 Orthogonality test of LasR-pLas-GFP </b> (i):Fluorescent response to cognate and  non-cognate AHLs (ii)Dose-Response curves for cognate and  non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL' /></p>

Revision as of 00:47, 1 November 2017

Experiment and Modeling

Testing Fluorescent AHL Receivers

Orthogonality test

Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct. <b>Fig. 2 Orthogonality test of LasR-pLas-GFP </b> (i):Fluorescent response to cognate and  non-cognate AHLs (ii)Dose-Response curves for cognate and  non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL