Difference between revisions of "Team:Cadets2Vets/Parts"

 
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{{Team:Cadets2Vets/SideMenu}}
 
{{Team:Cadets2Vets/SideMenu}}
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<html xmlns="http://www.w3.org/1999/xhtml">
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<html>
 
<head>
 
<head>
 
<title>
 
<title>
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Parts - USE THIS ONE!!!</title>
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Parts - Home</title>
 
<!-- META -->
 
<!-- META -->
 
<meta charset="UTF-8">
 
<meta charset="UTF-8">
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<meta name="keywords" content="Cadets2Vets, IGEM, igem, RAIN, biology">
 
 
 
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<link href="https://2017.igem.org/Team:Cadets2Vets/css/common?action=raw&ctype=text/css" rel="stylesheet">
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                <link href="https://2017.igem.org/Team:Cadets2Vets/css/all_css?action=raw&ctype=text/css" rel="stylesheet">
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<script src="https://www.youtube.com/iframe_api"></script>
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<link rel="icon" type="image/png" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
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<!-- CSS -->
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<link rel="apple-touch-icon" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
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<link rel="stylesheet" href="//edit.www.imcreator.com/css/common.css?v=1.4.1a">
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        <link href="https://2017.igem.org/Team:Cadets2Vets/css/parts/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
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<link rel="stylesheet" href="//edit.www.imcreator.com/js/lib/sweetalert/sweetalert.css" />
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<link href="https://2017.igem.org/Team:Cadets2Vets/css/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
−
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/all_css.css?v=1.4.1a" />
+
        <link href="https://2017.igem.org/Team:Cadets2Vets/css/fonts?action=raw&ctype=text/css" rel="stylesheet">
−
 
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                <link href="https://2017.igem.org/Team:Cadets2Vets/css/effects?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/lightbox?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/spimeview?action=raw&ctype=text/css" rel="stylesheet">
 +
        <link href="https://2017.igem.org/Team:Cadets2Vets/css/parts/static_style?action=raw&ctype=text/css" rel="stylesheet">
 +
<!-- SCRIPT -->
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/xprs_helper?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/all_js?action=raw&ctype=text/javascript"></script>
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                <script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lib/touchswipe/jquery_mobile_custom_min?action=raw&ctype=text/javascript"></script>
 
 
 
 
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<link rel="icon" type="image/png" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
 
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<link rel="apple-touch-icon" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
 
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<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
 
−
 
−
<link id="style-59a5b-k3uctfaa" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-k3uctfaa&viewer=xprs&parent_style=style-59a5b-e4hjr8q9&v=1.4.1a">
 
−
<link id="style-59a5b-lbaqmrpi" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-lbaqmrpi&viewer=xprs&parent_style=style-59a5b-e4hjr8q9&v=1.4.1a">
 
−
<link id="style-59a5b-jbqq3wgo" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-jbqq3wgo&viewer=xprs&parent_style=style-59a5b-e4hjr8q9&v=1.4.1a">
 
−
<link id="style-ebb8e3a7-iv52gu6d" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-ebb8e3a7-iv52gu6d&viewer=xprs&parent_style=style-59a5b-e4hjr8q9&v=1.4.1a">
 
−
<link id="style-59a5b-e4hjr8q9" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-e4hjr8q9&viewer=xprs&parent_style=style-59a5b-e4hjr8q9&v=1.4.1a">
 
−
<link id="style-59a5b-yamofliu" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-yamofliu&viewer=xprs&parent_style=style-59a5b-e4hjr8q9&v=1.4.1a">
 
−
<link id="vbid-59a5b-xtfijafb-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-59a5b-xtfijafb-STRIPE_DATA&v=1.4.1a">
 
−
 
−
<link id="vbid-59a5b-fnxcttvm-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-59a5b-fnxcttvm-STRIPE_DATA&v=1.4.1a">
 
−
 
−
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/fonts.css?v=1.4.1a" />
 
−
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/effects.css?v=1.4.1a" />
 
−
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/lightbox.css?v=1.4.1a">
 
−
 
−
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/spimeview.css?v=1.4.1a">
 
−
 
−
 
−
<!-- SCRIPT -->
 
−
<script src="https://code.jquery.com/jquery-2.x-git.min.js" type="text/javascript"></script>
 
−
<script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script>
 
−
 
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<script src="//edit.www.imcreator.com/js/xprs_helper.js?v=1.4.1a" type="text/javascript"></script>
 
−
 
−
<script src="//edit.www.imcreator.com/js/lib/jquery.cookie.min.js" type="text/javascript"></script>
 
−
<script src="//edit.www.imcreator.com/js/lib/sweetalert/sweetalert.min.js" type="text/javascript"></script>
 
−
<script src="//edit.www.imcreator.com/translation_js?lang=en" type="text/javascript"></script>
 
−
 
−
<!-- User Head Code -->
 
−
 
−
 
−
<script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.1a" type="text/javascript"></script>
 
−
 
−
<script type="text/javascript" src="//edit.www.imcreator.com/all_js.js?v=1.4.1a"></script>
 
−
<script src="//edit.www.imcreator.com/js/lib/touchswipe/jquery.mobile.custom.min.js"></script>
 
−
<script type="text/javascript">IMOS.pageView();</script>
 
−
 
−
 
−
 
−
 
 
 
 
<!-- User analytics -->
 
<!-- User analytics -->
−
 
−
<script type="text/javascript">
 
−
  var _gaq = _gaq || [];
 
−
  _gaq.push(['_setAccount', '']);
 
−
  _gaq.push(['_trackPageview']);
 
−
  (function() {
 
−
    var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true;
 
−
    ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js';
 
−
    var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s);
 
−
  })();
 
−
</script>
 
  
  
 +
<!-- Serving analytics -->
 +
<script>
 +
var currentDomain =  window.location.host;
 +
if (currentDomain != "localhost:7000"){
 +
var _gaq = _gaq || [];
 +
_gaq.push(['_setAccount', 'UA-58695071-1']);
 +
_gaq.push(['_setDomainName', currentDomain]);
 +
_gaq.push(['_setAllowLinker', true]);
 +
_gaq.push(['_trackPageview']);
 +
}
 +
</script>
 +
 
<!-- Label config-->
 
<!-- Label config-->
 
<script>
 
<script>
 
 
−
var LABEL_CONFIG = JSON.parse('{"LANDING_PAGE": "fast_homepage", "TIERS": {"silver": {"ALLOW_ECOMMERCE": "disallow", "ALLOW_BACKUPS": "allow", "ALLOW_BLOG": "allow", "PAGE_LIMIT": "unlimited"}, "gold": {"ALLOW_ECOMMERCE": "allow", "ALLOW_BACKUPS": "allow", "ALLOW_BLOG": "allow", "PAGE_LIMIT": "unlimited"}}, "BILLING": {"BUY_NOW_URL": "none", "BUY_NOW_IMAGE": "none", "PAYPAL_RECURRENCES": "1", "CUSTOM_CURRENCY": "USD", "HIDE_FREE_URL": "off", "PAYPAL_ID": "", "PAYPAL_AMOUNT": "100", "CUSTOM_TITLE": "Single Commercial License", "PAYPAL_PERIOD": "Y", "CUSTOM_EXPLAINED": "single domain connection for a single site. unlimited bandwidth, unlimited storage, premium support, e-commerce", "BILLING_TYPE": "abtest", "PAYPAL_OFFER_NAME": "Premium Subscription", "PAYPAL_EXPLAINED": "single domain connection for a single site. unlimited bandwidth, unlimited storage, premium support, e-commerce", "CUSTOM_CYCLE": "/month paid annualy", "PAYPAL_CURRENCY": "USD", "CUSTOM_PRICE": "100"}, "BRANDING": {"BRAND_THEMES_LOGO": "none", "BRAND_SECONDARY_COLOR": "rgb(255,255,255)", "BRAND_MAIN_COLOR": "#0099CC", "BRAND_BG": "https://lh3.googleusercontent.com/ZgLp2jeL7ynJJZ-aMv-LgcFLv1-wuV0E4mMxsQXqyPe9CDHiClIlQODrGLalwzQp4XNWjMBy8Z68369P4Q", "BUTTON_HOVER_COLOR": "#0099cc", "BRAND_SMALL_LOGO": "https://lh3.googleusercontent.com/PVw5qfegr3TcOhyx6UH2QShnmtTexxEIuzECuxiKnbfkzYPv28lrzSMSnelUe7nkoYjs-KLxOYzo1wgOSZk", "BRAND_LARGE_LOGO": "https://lh3.googleusercontent.com/BVPCuhW88_eSWMXpfFE8ZZh9IxRktAzfOp_rZ9vvhH2zLhLNoCJ6TjOleEnqppMxvYHdvsxDX-uXWfRrgQ"}, "SUPPORT": {"SUPPORT_URL": "http://support.imxprs.com/hc/en-us/requests/new", "VIDEO_ID": "K1ZnSLe-Rdk", "SUPPORT_ID": "", "KNOWLEDGE_CENTER_URL": "http://support.imxprs.com/hc/en-us"}, "ANALYTICS_ID": "UA-64970204-1", "META": {"OWNER": "xprsxprs", "DOMAIN": "imcreator.com", "CREATION_DATE": "28-07-2015 13:34:42", "PRODUCT_NAME": "XPRS", "NAME": "xprs"}, "DOMAIN_BILLING": {"BUY_NOW_URL": "none", "PAYPAL_RECURRENCES": "1", "CUSTOM_CURRENCY": "USD", "PAYPAL_PERIOD": "Y", "PAYPAL_AMOUNT": "100", "PAYPAL_ID": "", "CUSTOM_EXPLAINED": "1-Year domain subscription", "BILLING_TYPE": "standard", "PAYPAL_OFFER_NAME": "Premium Subscription", "PAYPAL_CURRENCY": "USD", "CUSTOM_PRICE": "100"}, "ID": "label-f6d7c", "SETTINGS": {"ALLOW_PERIPHERAL_PAGES": "allow", "LOGIN_URL": "", "ALLOW_ELEMENTS": "allow", "ALLOW_ECOMMERCE": "allow", "ALLOW_NEW_DOMAIN": "allow", "ENABLE_IMXPRT": "true", "ECOMMERCE_DASHBOARD": "http://dashboard.shoprocket.co", "INCLUDE_REGISTER_PHONE": "disallow", "CUSTOM_TEMPLATES": "default", "ALLOW_CALL_ME_CHECKBOX": "disallow", "SR_PARTNER_KEY": "N/A", "PHONE_EDIT_APP_LINK": "https://itunes.apple.com/app/apple-store/id953999749?pt=1847141&mt=8", "DEFAULT_EDITOR_LANG": "en", "CUSTOM_SECTIONS": "default", "ALLOW_PHONE_EDIT": "allow", "ALLOW_BACKUPS": "allow", "ALLOW_NEW_SITES": "allow", "REGISTER_URL": "", "BILLING_SERVER": "", "USER_PROFILE": "show", "REGISTER_STEP": "themes", "ENABLE_IMOS": "false", "ECOMMERCE_SOLUTION": "IMOS", "WEBHOOKS_SERVER": "", "ALLOW_RESELLER": "allow"}, "HIY": "false", "EMAILS": {"FORWARD_ALL_EMAILS": "off", "FORWARD_EMAIL": ""}, "SERVERS": {"HEROKU_APP": "", "S3_ACCESS": "", "S3_REGION": "", "UPLOAD_TO_S3": "off", "S3_SECRET": "", "S3_BUCKET": ""}, "TOS_URL": "http://www.imcreator.com/terms-of-service"}');
+
var LABEL_CONFIG = {};
 
 
 
 
 
</script></head>
 
</script></head>
  
−
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.1a" data-caller="preview"  data-ecommerce-dashboard="http://dashboard.shoprocket.co" data-static-server="//edit.www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
+
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.1f" data-caller="live"  data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 
 
−
<div id="xprs" data-website-name="USE THIS ONE!!!" class="xprs-holder" >
+
<div id="xprs" data-website-name="Home" class="xprs-holder" >
 
<div class="main-page "  >
 
<div class="main-page "  >
 
<div id="content">
 
<div id="content">
−
<div  id="vbid-59a5b-xtfijafb"  class="master container style-59a5b-e4hjr8q9 content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Parts"  data-itemslug="parts" data-itemstyleid="style-59a5b-e4hjr8q9" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-59a5b-xtfijafb" data-preset-type-id="ITEMS" data-preview-style="style-59a5b-e4hjr8q9" data-style="style-5a24e-kzdyurm3ju" data-absolute-path="http://edit.www.imcreator.com/viewer"  >
+
<div  id="vbid-d1ee0827-46fj81we"  class="master container style-d1ee0827-c6y28b4l content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Parts"  data-itemslug="parts" data-itemstyleid="style-d1ee0827-c6y28b4l" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-d1ee0827-46fj81we" data-preset-type-id="ITEMS" data-preview-style="style-d1ee0827-c6y28b4l" data-style="style-5a24e-kzdyurm3ju" data-absolute-path=""  >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-466a74f8-j2jzxizg" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="index.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="b951bd16"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook</span>
+
<span id="element-a1a56421418f7ce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-5515b55f-jgjhhkan" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="collaborations.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="928ba103"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
+
<span id="element-5efad87d43e4352"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-9e25704e-ynxs9cit" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="description.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="e08666cf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook-July</span>
+
<span id="element-e6e2afb357139e4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Description</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-xtfijafb" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="design.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="bd841f1b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
+
<span id="element-c6287513fca8901"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewerhttp://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-xtfijafb" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="experiments.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-xtfijafb"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
+
<span id="element-f4b485a3e8b3825"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-603d2303312c4bd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-august.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-e39fe0beffa33cd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook August</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-july.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-09ca318ecaf1b2f"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook July</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-june.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-367041e6445b247"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook June</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="public-engagement.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-307cb61a9a844e1"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="safety.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-fa38138e1dd4fce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
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<a class="removable-parent" href="composite-parts.html" data-link-type="EXISTING"  target="_self" >
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 +
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<a class="removable-parent" href="interlab.html" data-link-type="EXISTING"  target="_self" >
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<a class="removable-parent" href="environmental-project.html" data-link-type="EXISTING"  target="_self" >
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<a class="removable-parent" href="hardware.html" data-link-type="EXISTING"  target="_self" >
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<span id="8d3cfa8c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Hardware</span>
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</a>
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<a class="removable-parent" href="demonstrate.html" data-link-type="EXISTING"  target="_self" >
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<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
 +
</a>
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<a class="removable-parent" href="improve.html" data-link-type="EXISTING"  target="_self" >
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<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
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<span id="7e0ed437"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Results</span>
 +
</a>
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</li>
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<a class="removable-parent" href="attribution.html" data-link-type="EXISTING"  target="_self" >
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<span id="8a69633b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Attribution</span>
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<span id="643d5085"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span>
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<a class="removable-parent" href="silver-hp.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-563894f97a8ebaf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Silver HP</span>
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<div  id="vbid-ba13a303-pwfs3s8z"  class="master item-box  gallery-box style-ba13a303-cfykn4og    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-ba13a303-cfykn4og" data-preview-styleid='style-ba13a303-cfykn4og' data-preset-type-id="TEXT_BLOCK">
 
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<div  class="sub container style-ba13a303-cfykn4og content flex   " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-ba13a303-cfykn4og" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-ba13a303-pwfs3s8z" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-ba13a303-cfykn4og" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 
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<h2 id="vbid-bf16b63b-e84ew9yn" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >ARS R Regulatory Protein</h2>
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<h1 id="vbid-ba13a303-lhrwnuia" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >circuit parts overview<br></h1>
 
 
 
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<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed malesuada faucibus ex nec ultricies. Donec mattis egestas nisi non pretium. Suspendisse nec eros ut erat facilisis maximus. In congue et leo in varius. Vestibulum sit amet felis ornare, commodo orci ut, feugiat lorem.</p>
+
<p>Cadets2Vets has built a biological arsenic circuit that uses a combination of native DH5-alpha E. coli genes to detect arsenic ions in solution and genetic elements capable of producing GFP (Green Fluorescent Protein). After reading through academic literature, our team found a negative repressor that could be used as an arsenic regulator known as ArsR. Sensitive to arsenic ions, this protein will bind to an operon or promoter sequence and inhibit the transcription of the downstream gene in the presence of a transcription factor (in our case arsenic). Expressing a conformation change, this strategy allows us to control GFP expression using a protein sensitive to and capable of bonding with arsenic ions in solution.</p><p><br></p><p>Integrated DNA Technologies, an iGEM sponsor, provided us with free synthetic DNA to build our circuit. This sponsorship allowed us to select promoters and genes that fit our project. The molecular cloning strategy was to request two GBlocks that would contain a promoter-ribosome binding site-CDS-terminator sequence for constituitively expressing ArsR and regulating the expression of GFP via ArsR. We also added in a restriction enzyme site to both GBlocks that enabled us to combine the two GBlocks together.</p>
 
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<div class="gallery-wrapper item-wrapper projects-wrapper ">
 
 
−
<!-- CACHED VERSION (7) OF vbid-59a5b-tavwjrqq FROM  24-07-2017 23:00:23 --><div  class="sub container style-59a5b-k3uctfaa content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-59a5b-k3uctfaa" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-59a5b-tavwjrqq" data-preset-type-id="PROJECTS" data-preview-style="style-59a5b-k3uctfaa" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path="https://xprs.imcreator.com/viewer"  >
+
<div  class="sub container style-d1ee0827-r7hdefvx content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-d1ee0827-r7hdefvx" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-d1ee0827-m6jrgnhp" data-preset-type-id="PROJECTS" data-preview-style="style-d1ee0827-r7hdefvx" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path=""  >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
Line 489: Line 629:
 
<!-- STRIPE HEADER -->
 
<!-- STRIPE HEADER -->
 
<div class="stripe-header-wrapper">
 
<div class="stripe-header-wrapper">
−
<div  id="vbid-59a5b-4sxu2tdq"  class="stripe-header sub item-box page-box style-59a5b-k3uctfaa       " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-k3uctfaa" data-preview-styleid='style-59a5b-k3uctfaa' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-d1ee0827-ebftbm5y"  class="stripe-header sub item-box page-box style-d1ee0827-r7hdefvx       " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-d1ee0827-r7hdefvx" data-preview-styleid='style-d1ee0827-r7hdefvx' data-preset-type-id="UNRESOLVED">
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
−
<div class="item-content leaf blocks_layout page content" data-self="vbid-59a5b-4sxu2tdq" data-preview-style="style-59a5b-k3uctfaa" data-style="style-8ec4c-t1avbp6mmi" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-4sxu2tdq" data-bgimg="">
+
<div class="item-content leaf blocks_layout page content" data-self="vbid-d1ee0827-ebftbm5y" data-preview-style="style-d1ee0827-r7hdefvx" data-style="style-8ec4c-t1avbp6mmi" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-d1ee0827-ebftbm5y" data-bgimg="">
 
<div class="helper-div middle" >
 
<div class="helper-div middle" >
 
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 
<!-- <div class="benet" style="min-height:inherit;"></div> -->
Line 505: Line 645:
 
<div class="blocks-preview-title-holder removable-parent order-handle">
 
<div class="blocks-preview-title-holder removable-parent order-handle">
 
 
−
<h2 id="vbid-59a5b-qjsedtuk" class="preview-element blocks-preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_TITLE" >Existing PArts</h2>
+
<h2 id="vbid-d1ee0827-ral3lavl" class="preview-element blocks-preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_TITLE" >Our PArts</h2>
 
 
 
</div>
 
</div>
Line 535: Line 675:
 
 
 
 
−
<div  id="vbid-cb51050e-j5ixjhxk"  class="sub item-box  page-box style-59a5b-k3uctfaa         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-k3uctfaa" data-preview-styleid='style-59a5b-k3uctfaa' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-d1ee0827-cv7mrsdp"  class="sub item-box  page-box style-d1ee0827-r7hdefvx         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-d1ee0827-r7hdefvx" data-preview-styleid='style-d1ee0827-r7hdefvx' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-cb51050e-j5ixjhxk" data-preview-style="style-59a5b-k3uctfaa" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="3456" data-orig-thumb-width="5184" data-vbid="vbid-cb51050e-j5ixjhxk" data-bgimg="https://lh3.googleusercontent.com/j835qeUr9bs2YyV_nI6qH24BM9SG9v1dqEF1FuCJCboyXnx0Pws7TVIz-KYy3dEomb_fCvrKK8xT3miAgQ">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-d1ee0827-cv7mrsdp" data-preview-style="style-d1ee0827-r7hdefvx" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1000" data-orig-thumb-width="1380" data-vbid="vbid-d1ee0827-cv7mrsdp" data-bgimg="https://static.igem.org/mediawiki/2017/8/8b/C2V_Parts_SalILinkerRestrictionSite.png">
 
<div  class="multi-container preview image-cover" >
 
<div  class="multi-container preview image-cover" >
 
<div class="Picture item-preview">
 
<div class="Picture item-preview">
Line 549: Line 689:
 
<div class="pic-side">
 
<div class="pic-side">
 
<div class="vertical-aligner">
 
<div class="vertical-aligner">
−
<div id="vbid-cb51050e-mkospn34-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+
<div id="vbid-d1ee0827-rbus9ziy-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<a class="image-link top-layer not-wrapping" href="http://parts.igem.org/Part:BBa_J23102"  data-link-type="EXTERNAL" target="_blank" ></a>
+
<a class="image-link top-layer not-wrapping" href="http://parts.igem.org/Part:BBa_K2248000"  data-link-type="EXTERNAL" target="_blank" ></a>
  
−
<div  id="vbid-cb51050e-mkospn34" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/j835qeUr9bs2YyV_nI6qH24BM9SG9v1dqEF1FuCJCboyXnx0Pws7TVIz-KYy3dEomb_fCvrKK8xT3miAgQ=s300);"  data-orig-width="5184" data-orig-height="3456" >
+
<div  id="vbid-d1ee0827-rbus9ziy" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/8/8b/C2V_Parts_SalILinkerRestrictionSite.png);"  data-orig-width="1380" data-orig-height="1000" >
 
<!-- VIDEO TEMPLATE -->
 
<!-- VIDEO TEMPLATE -->
 
 
Line 592: Line 732:
 
<div class="preview-title-holder removable-parent order-handle">
 
<div class="preview-title-holder removable-parent order-handle">
 
 
−
<a href="http://parts.igem.org/Part:BBa_J23102"  data-link-type="EXTERNAL" target="_blank" >
+
<a href="http://parts.igem.org/Part:BBa_K2248000"  data-link-type="EXTERNAL" target="_blank" >
 
 
−
<h2 id="vbid-cb51050e-bsppibva" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Constitutive  E. coli    promoter</h2>
+
<h2 id="vbid-d1ee0827-ldivvjwr" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >SalI Linker Restriction Site</h2>
 
</a>
 
</a>
 
</div>
 
</div>
Line 614: Line 754:
 
<div class="preview-body-holder removable-parent order-handle">
 
<div class="preview-body-holder removable-parent order-handle">
 
 
−
<a href="http://parts.igem.org/Part:BBa_J23102"  data-link-type="EXTERNAL" target="_blank" >
+
<a href="http://parts.igem.org/Part:BBa_K2248000"  data-link-type="EXTERNAL" target="_blank" >
 
 
−
<div id="vbid-cb51050e-ezxrsln7" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+
<div id="vbid-d1ee0827-usb41req" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
−
<p><span style="font-size:11px;"><span style="font-family:arial;">Promoter: <span id="docs-internal-guid-f65cecb1-671c-31b3-0c08-cbf5594b554b"><span style="color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">BBa_J23102</span></span></span></span></p>
+
<p>BBa_K2248000</p>
 
</div>
 
</div>
 
</a>
 
</a>
Line 637: Line 777:
  
 
 
−
<a class="removable-parent" href="http://parts.igem.org/Part:BBa_J23102" data-link-type="EXTERNAL"  target="_blank" >
+
<a class="removable-parent" href="http://parts.igem.org/Part:BBa_K2248000" data-link-type="EXTERNAL"  target="_blank" >
 
 
−
<span id="vbid-cb51050e-vjqly0qj"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
+
<span id="vbid-d1ee0827-e7htftnd"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
 
</a>
 
</a>
  
Line 679: Line 819:
 
 
 
 
−
<div  id="vbid-59a5b-dgfpsgjy"  class="sub item-box  page-box style-59a5b-k3uctfaa         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-k3uctfaa" data-preview-styleid='style-59a5b-k3uctfaa' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-e22d8bff-opvd7oao"  class="sub item-box  page-box style-d1ee0827-r7hdefvx         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-d1ee0827-r7hdefvx" data-preview-styleid='style-d1ee0827-r7hdefvx' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-dgfpsgjy" data-preview-style="style-59a5b-k3uctfaa" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1733" data-orig-thumb-width="2600" data-vbid="vbid-59a5b-dgfpsgjy" data-bgimg="https://lh3.googleusercontent.com/f6U5Lc7seDemCXzJ6s2bd494F9E_l5G5-cjLL55PNUytAnW7H6CqvAEvxaZs7ifD-2oOP9DFdZiw3d5Jzw">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-e22d8bff-opvd7oao" data-preview-style="style-d1ee0827-r7hdefvx" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="525" data-orig-thumb-width="544" data-vbid="vbid-e22d8bff-opvd7oao" data-bgimg="https://static.igem.org/mediawiki/2017/b/ba/C2V_Parts_BiosensorArs2.png">
 
<div  class="multi-container preview image-cover" >
 
<div  class="multi-container preview image-cover" >
 
<div class="Picture item-preview">
 
<div class="Picture item-preview">
Line 693: Line 833:
 
<div class="pic-side">
 
<div class="pic-side">
 
<div class="vertical-aligner">
 
<div class="vertical-aligner">
−
<div id="vbid-59a5b-uvqmxyij-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+
<div id="vbid-e22d8bff-wths8jko-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<a class="image-link top-layer not-wrapping" href="http://parts.igem.org/Part:BBa_J61100"  data-link-type="EXTERNAL" target="_blank" ></a>
+
<a class="image-link top-layer not-wrapping" href="http://parts.igem.org/Part:BBa_K2248003"  data-link-type="EXTERNAL" target="_blank" ></a>
  
−
<div  id="vbid-59a5b-uvqmxyij" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/f6U5Lc7seDemCXzJ6s2bd494F9E_l5G5-cjLL55PNUytAnW7H6CqvAEvxaZs7ifD-2oOP9DFdZiw3d5Jzw=s300);"  data-orig-width="2600" data-orig-height="1733" >
+
<div  id="vbid-e22d8bff-wths8jko" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/b/ba/C2V_Parts_BiosensorArs2.png);"  data-orig-width="544" data-orig-height="525" >
 
<!-- VIDEO TEMPLATE -->
 
<!-- VIDEO TEMPLATE -->
 
 
Line 736: Line 876:
 
<div class="preview-title-holder removable-parent order-handle">
 
<div class="preview-title-holder removable-parent order-handle">
 
 
−
<a href="http://parts.igem.org/Part:BBa_J61100"  data-link-type="EXTERNAL" target="_blank" >
+
<a href="http://parts.igem.org/Part:BBa_K2248003"  data-link-type="EXTERNAL" target="_blank" >
 
 
−
<h2 id="vbid-59a5b-kjktjjco" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >RBS</h2>
+
<h2 id="vbid-e22d8bff-au235fzu" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Biosensor: Ars2.0</h2>
 
</a>
 
</a>
 
</div>
 
</div>
Line 758: Line 898:
 
<div class="preview-body-holder removable-parent order-handle">
 
<div class="preview-body-holder removable-parent order-handle">
 
 
−
<a href="http://parts.igem.org/Part:BBa_J61100"  data-link-type="EXTERNAL" target="_blank" >
+
<a href="http://parts.igem.org/Part:BBa_K2248003"  data-link-type="EXTERNAL" target="_blank" >
 
 
−
<div id="vbid-59a5b-euuitr22" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+
<div id="vbid-e22d8bff-4cfbkvar" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
−
<p><span style="font-size:11px;">Ribosome Binding Site: <span id="docs-internal-guid-f65cecb1-671c-bff4-5a3f-2cb2966b25a8"><span style="color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">BBa_J61100</span></span></span></p>
+
<p>BBa_K2248003</p>
 
</div>
 
</div>
 
</a>
 
</a>
Line 781: Line 921:
  
 
 
−
<a class="removable-parent" href="http://parts.igem.org/Part:BBa_J61100" data-link-type="EXTERNAL"  target="_blank" >
+
<a class="removable-parent" href="http://parts.igem.org/Part:BBa_K2248003" data-link-type="EXTERNAL"  target="_blank" >
 
 
−
<span id="vbid-59a5b-zobzlhha"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
+
<span id="vbid-e22d8bff-ub3mbr5k"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
 
</a>
 
</a>
  
Line 823: Line 963:
 
 
 
 
−
<div  id="vbid-2d3e866a-o1ourue0"  class="sub item-box  page-box style-59a5b-k3uctfaa         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-k3uctfaa" data-preview-styleid='style-59a5b-k3uctfaa' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-d1ee0827-s5uasg51"  class="sub item-box  page-box style-d1ee0827-r7hdefvx         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-d1ee0827-r7hdefvx" data-preview-styleid='style-d1ee0827-r7hdefvx' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-2d3e866a-o1ourue0" data-preview-style="style-59a5b-k3uctfaa" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="3456" data-orig-thumb-width="5184" data-vbid="vbid-2d3e866a-o1ourue0" data-bgimg="https://lh3.googleusercontent.com/j835qeUr9bs2YyV_nI6qH24BM9SG9v1dqEF1FuCJCboyXnx0Pws7TVIz-KYy3dEomb_fCvrKK8xT3miAgQ">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-d1ee0827-s5uasg51" data-preview-style="style-d1ee0827-r7hdefvx" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="230" data-orig-thumb-width="317" data-vbid="vbid-d1ee0827-s5uasg51" data-bgimg="https://static.igem.org/mediawiki/2017/1/11/C2V_Parts_ARSRRegulatoryProteinPcArsR.gif">
 
<div  class="multi-container preview image-cover" >
 
<div  class="multi-container preview image-cover" >
 
<div class="Picture item-preview">
 
<div class="Picture item-preview">
Line 837: Line 977:
 
<div class="pic-side">
 
<div class="pic-side">
 
<div class="vertical-aligner">
 
<div class="vertical-aligner">
−
<div id="vbid-2d3e866a-ox8znmjl-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+
<div id="vbid-d1ee0827-gajgul6y-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div  id="vbid-2d3e866a-ox8znmjl" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/j835qeUr9bs2YyV_nI6qH24BM9SG9v1dqEF1FuCJCboyXnx0Pws7TVIz-KYy3dEomb_fCvrKK8xT3miAgQ=s300);"  data-orig-width="5184" data-orig-height="3456" >
+
<a class="image-link top-layer not-wrapping" href="http://parts.igem.org/Part:BBa_K2248001"  data-link-type="EXTERNAL" target="_blank" ></a>
 +
 
 +
<div  id="vbid-d1ee0827-gajgul6y" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/1/11/C2V_Parts_ARSRRegulatoryProteinPcArsR.gif);"  data-orig-width="317" data-orig-height="230" >
 
<!-- VIDEO TEMPLATE -->
 
<!-- VIDEO TEMPLATE -->
 
 
Line 878: Line 1,020:
 
<div class="preview-title-holder removable-parent order-handle">
 
<div class="preview-title-holder removable-parent order-handle">
 
 
−
<h2 id="vbid-2d3e866a-7xstx9ib" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >GFP</h2>
+
<a href="http://parts.igem.org/Part:BBa_K2248001"  data-link-type="EXTERNAL" target="_blank" >
 
 
 +
<h2 id="vbid-d1ee0827-6dxz4jzx" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >ARS R Regulatory Protein: PcArsR</h2>
 +
</a>
 
</div>
 
</div>
 
<br class="lower-line-break" />
 
<br class="lower-line-break" />
Line 898: Line 1,042:
 
<div class="preview-body-holder removable-parent order-handle">
 
<div class="preview-body-holder removable-parent order-handle">
 
 
−
<div id="vbid-2d3e866a-lqr0iyxg" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+
<a href="http://parts.igem.org/Part:BBa_K2248001"  data-link-type="EXTERNAL" target="_blank" >
−
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed malesuada faucibus ex nec ultricies. Donec mattis egestas nisi non pretium. Suspendisse nec eros ut erat facilisis maximus. In congue et leo in varius. Vestibulum sit amet felis ornare, commodo orci ut, feugiat lorem.</p>
+
−
</div>
+
 
 
 +
<div id="vbid-d1ee0827-bsa85a5k" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p>BBa_K2248001</p>
 +
</div>
 +
</a>
 
</div>
 
</div>
 
<br class="lower-line-break" />
 
<br class="lower-line-break" />
Line 919: Line 1,065:
  
 
 
−
<span id="vbid-2d3e866a-9y0s0kxi"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
+
<a class="removable-parent" href="http://parts.igem.org/Part:BBa_K2248001" data-link-type="EXTERNAL"  target="_blank" >
−
+
 +
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<p><span style="font-size:11px;">Terminator: <span id="docs-internal-guid-f65cecb1-671d-045e-ec96-8765fcfdce77"><span style="color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">BBa_B0015</span></span></span></p>
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<groupparts>iGEM17 Cadets2Vets</groupparts>
 
<groupparts>iGEM17 Cadets2Vets</groupparts>

Latest revision as of 02:20, 1 November 2017

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circuit parts overview





Cadets2Vets has built a biological arsenic circuit that uses a combination of native DH5-alpha E. coli genes to detect arsenic ions in solution and genetic elements capable of producing GFP (Green Fluorescent Protein). After reading through academic literature, our team found a negative repressor that could be used as an arsenic regulator known as ArsR. Sensitive to arsenic ions, this protein will bind to an operon or promoter sequence and inhibit the transcription of the downstream gene in the presence of a transcription factor (in our case arsenic). Expressing a conformation change, this strategy allows us to control GFP expression using a protein sensitive to and capable of bonding with arsenic ions in solution.


Integrated DNA Technologies, an iGEM sponsor, provided us with free synthetic DNA to build our circuit. This sponsorship allowed us to select promoters and genes that fit our project. The molecular cloning strategy was to request two GBlocks that would contain a promoter-ribosome binding site-CDS-terminator sequence for constituitively expressing ArsR and regulating the expression of GFP via ArsR. We also added in a restriction enzyme site to both GBlocks that enabled us to combine the two GBlocks together.


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2304 Jefferson Ave, Tacoma, WA 98402


<groupparts>iGEM17 Cadets2Vets</groupparts>