Difference between revisions of "Team:Cadets2Vets/Parts"

 
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 +
{{Team:Cadets2Vets/SideMenu}}
 +
 
<html>
 
<html>
−
<script>
+
<head>
 +
<title>
 +
Parts - Home</title>
 +
<!-- META -->
 +
<meta charset="UTF-8">
 +
<meta name="viewport" content="width=device-width, maximum-scale=1"/>  
 
 
−
// This is the jquery part of your template.
+
−
// Try not modify any of this code too much since it makes your menu work.
+
−
$(document).ready(function() {
+
<link href="https://2017.igem.org/Team:Cadets2Vets/css/common?action=raw&ctype=text/css" rel="stylesheet">
−
$("#HQ_page").attr('id','');
+
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/all_css?action=raw&ctype=text/css" rel="stylesheet">
−
// call the functions that control the menu
+
<link rel="icon" type="image/png" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
−
menu_functionality();
+
<link rel="apple-touch-icon" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
−
hide_show_menu();
+
        <link href="https://2017.igem.org/Team:Cadets2Vets/css/parts/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
−
//this function controls the expand and collapse buttons of the menu and changes the +/- symbols
+
<link href="https://2017.igem.org/Team:Cadets2Vets/css/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
−
function menu_functionality() {
+
        <link href="https://2017.igem.org/Team:Cadets2Vets/css/fonts?action=raw&ctype=text/css" rel="stylesheet">
−
//when clicking on a "menu_button", it will change the "+/-" accordingly and it will show/hide the corresponding submenu
+
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/effects?action=raw&ctype=text/css" rel="stylesheet">
−
$(".menu_button").click(function(){
+
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/lightbox?action=raw&ctype=text/css" rel="stylesheet">
−
// add or remove the class "open" , this class holds the "-"  
+
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/spimeview?action=raw&ctype=text/css" rel="stylesheet">
−
$(this).children().toggleClass("open");
+
        <link href="https://2017.igem.org/Team:Cadets2Vets/css/parts/static_style?action=raw&ctype=text/css" rel="stylesheet">
−
+
<!-- SCRIPT -->
−
// show or hide the submenu
+
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/xprs_helper?action=raw&ctype=text/javascript"></script>
−
$(this).next('.submenu_wrapper').fadeToggle(400);
+
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/all_js?action=raw&ctype=text/javascript"></script>
−
});
+
                <script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lib/touchswipe/jquery_mobile_custom_min?action=raw&ctype=text/javascript"></script>
−
// when the screen size is smaller than 800px, the display_menu_control button appears and will show/hide the whole menu
+
−
$("#display_menu_control").click(function(){
+
−
$('#menu_content').fadeToggle(400);
+
−
});
+
<!-- User analytics -->
−
// call the current page highlight function
+
 
−
highlight_current_page();
+
 
 +
<!-- Serving analytics -->
 +
<script>
 +
var currentDomain =  window.location.host;
 +
if (currentDomain != "localhost:7000"){
 +
var _gaq = _gaq || [];
 +
_gaq.push(['_setAccount', 'UA-58695071-1']);
 +
_gaq.push(['_setDomainName', currentDomain]);
 +
_gaq.push(['_setAllowLinker', true]);
 +
_gaq.push(['_trackPageview']);
 
}
 
}
−
// call the highlight current page function to show it on the menu with a different background color
+
</script>
−
function highlight_current_page() {
+
−
// select a page from the menu based on the id assigned to it and the current page name and add the class "current page" to make it change background color
+
<!-- Label config-->
−
$("#"+ wgPageName.substring(wgPageName.lastIndexOf("/")+1, wgPageName.length ) + "_page").addClass("current_page");
+
<script>
−
// now that the current_page class has been added to a menu item, make the submenu fade in
+
−
$(".current_page").parents(".submenu_wrapper").fadeIn(400);
+
var LABEL_CONFIG = {};
 +
 +
</script></head>
 +
 
 +
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.1f" data-caller="live"  data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 +
 +
<div id="xprs" data-website-name="Home" class="xprs-holder" >
 +
<div class="main-page "  >
 +
<div id="content">
 +
<div  id="vbid-d1ee0827-46fj81we"  class="master container style-d1ee0827-c6y28b4l content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Parts"  data-itemslug="parts" data-itemstyleid="style-d1ee0827-c6y28b4l" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-d1ee0827-46fj81we" data-preset-type-id="ITEMS" data-preview-style="style-d1ee0827-c6y28b4l" data-style="style-5a24e-kzdyurm3ju" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="stripes"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="middle"></div>
 +
<div id="children">
 +
 +
 +
 +
<!-- MENUS START -->
 +
<div  id="vbid-59a5b-1xbnq2ek"  class="master item-box  header-box style-59a5b-jbqq3wgo injected        " data-holder-type="header"" data-holder-type="header"  data-child-type="ORIGIN"  data-styleid="style-59a5b-jbqq3wgo" data-preview-styleid='style-59a5b-jbqq3wgo' data-preset-type-id="MENUS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="header-wrapper item-wrapper menus-wrapper ">
 +
 +
<div class="item-content leaf menu_layout header content" data-preview-style="style-59a5b-jbqq3wgo" data-style="style-7f715-scjjarqgxu"  data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-1xbnq2ek" data-bgimg="">
 +
<div class="preview-content-wrapper">
 +
<div class="preview-content-holder">
 +
<div class="left-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="logo-holder">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
</div>
 +
<div class="helper-div">
 +
<div class="item-details menu">
 +
<!-- TITLE TEMPLATE -->
 
 
−
// change the +/- symbol of the corresponding menu button
+
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
−
$(".current_page").parents(".submenu_wrapper").prev().children().toggleClass("open");
+
 
−
}
+
<!-- SUBTITLE TEMPLATE -->
−
// allow button on the black menu bar to show/hide the side menu
+
−
function hide_show_menu() {
+
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 
 +
</div>
 +
</div>
 +
</div>
 +
<div class="right-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<!-- LINKS TEMPLATE -->
 +
<div class="preview-item-links ">
 +
<ul class="preview-links-wrapper">
 +
 
 +
 
 
 
−
// in case you preview mode is selected, the menu is hidden for better visibility
+
−
if (window.location.href.indexOf("submit") >= 0) {
+
<li class="removable-parent">
−
$(".igem_2017_menu_wrapper").hide();
+
−
}
+
<a class="removable-parent" href="index.html" data-link-type="EXISTING"   target="_self" >
−
// if the black menu bar has been loaded
+
−
  if (document.getElementById('bars_item')) {
+
<span id="element-a1a56421418f7ce" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
−
// when the "bars_item" has been clicked
+
</a>
−
$("#bars_item").click(function() {
+
−
$("#sideMenu").hide();
+
</li>
−
// show/hide the menu wrapper
+
 
−
$(".igem_2017_menu_wrapper").fadeToggle("100");
+
−
});
+
−
  }
+
−
// because the black menu bars loads at a different time than the rest of the page, this function is set on a time out so it can run again in case it has not been loaded yet
+
−
else {
+
−
    setTimeout(hide_show_menu, 15);
+
−
}
+
−
}
+
−
});
+
−
</script>
+
−
<style>
+
−
/***************************************************** DEFAULT WIKI SETTINGS ****************************************************/
+
−
/* Clear the default wiki settings */
+
−
#home_logo, #sideMenu { display:none; }
+
−
#sideMenu, #top_title, .patrollink  {display:none;}
+
−
body {background-color:white; }
+
−
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
−
/**************************************************************** MENU ***************************************************************/
+
−
/* Wrapper for the menu */
+
−
.igem_2017_menu_wrapper {
+
−
width: 15%;
+
−
height:100vh;
+
−
position:fixed;
+
−
right:0%;
+
−
padding:0px;
+
−
float:right;
+
−
border-left: 1px solid #383838;
+
−
background-color:#7c6429;
+
−
text-align:left;
+
−
font-family:Tahoma, Geneva, sans-serif;
+
−
overflow-y: auto;
+
−
overflow-x: hidden;
+
−
}
+
−
/* this hides the scrollbar to keep view consistency */
+
−
.igem_2017_menu_wrappe::-webkit-scrollbar {
+
−
display: none;
+
−
}
+
 
 
 
 
−
/* styling for links in the menu, removes the line under text */
+
<li class="removable-parent">
−
.igem_2017_menu_wrapper a { 
+
−
text-decoration: none;
+
<a class="removable-parent" href="collaborations.html" data-link-type="EXISTING"  target="_self" >
−
}
+
−
/* styling for the images in the menu */
+
<span id="element-5efad87d43e4352"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
−
.igem_2017_menu_wrapper img {
+
</a>
−
width: 100%;
+
−
}
+
−
/* styling for the menu buttons */
+
−
.igem_2017_menu_wrapper .menu_button {
+
−
width: 100%;
+
−
padding: 10px 0px 10px 15px;
+
−
float:left;
+
−
border-bottom: 1px solid #383838;
+
−
font-size: 12px;
+
−
font-weight: bold;
+
−
color: #ffffff;
+
−
cursor: pointer;
+
−
}
+
−
.igem_2017_menu_wrapper .menu_bottom_padding {
+
−
width: 100%;
+
−
height: 30px;
+
−
float:left;
+
−
}
+
−
.menu_button.direct_to_page {
+
−
padding-left: 36px;
+
−
}
+
−
.igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
+
−
width:10%;
+
−
float:left;
+
−
}
+
−
.igem_2017_menu_wrapper .menu_button .expand_collapse_icon::before {
+
−
content: "+"; 
+
−
}
+
 
 
−
.open::before {
+
</li>
−
content: "-" !important; 
+
 
−
}
+
 
 
−
/* styling for the menu buttons on hover */
+
−
.igem_2017_menu_wrapper .menu_button:hover, .igem_2017_menu_wrapper .submenu_button:hover ,  .submenu_button.current_page:hover {
+
<li class="removable-parent">
−
background-color: #3399ff;
+
−
text-decoration: none;
+
<a class="removable-parent" href="description.html" data-link-type="EXISTING"  target="_self" >
−
color:#ffffff;
+
−
}
+
<span id="element-e6e2afb357139e4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Description</span>
−
/* styling for the menu button when it is the current page */
+
</a>
−
.current_page {
+
−
background-color:#7fc1f7  !important;
+
−
color:#ffffff !important;
+
−
}
+
−
/* styling for the submenu buttons */
+
−
.igem_2017_menu_wrapper .submenu_button {
+
−
width: 100%;
+
−
padding: 10px 0px 10px 34px;
+
−
float:left;
+
−
background-color:#7c6429;
+
−
border-bottom: 1px solid #383838;
+
−
font-size: 12px;
+
−
color: #ffffff;
+
−
cursor: pointer;
+
−
}
+
−
/* wrapper for the submenu items, they are hidden by default*/
+
−
.igem_2017_menu_wrapper .submenu_wrapper {
+
−
display:none;
+
−
}
+
−
/* when the page size is bigger than 800px, this show/hide control is hidden by default */
+
−
.igem_2017_menu_wrapper #display_menu_control {
+
−
display:none;
+
−
text-align:center;
+
−
}
+
 
 
−
/***************************************************** CONTENT OF THE PAGE ****************************************************/
+
</li>
−
/* Wrapper for the content */
+
 
−
.igem_2017_content_wrapper {
+
−
width: 81%;
+
−
margin: 2%;
+
−
display:block;
+
−
float:left;
+
−
background-color:white;
+
−
font-family:Tahoma, Geneva, sans-serif;
+
−
}
+
−
/********************************* HTML STYLING  *********************************/
+
−
/* styling for the titles h1 h2 */
+
−
.igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 {
+
−
padding:5px 15px;
+
−
border-bottom: 0px;
+
−
color: #3399ff;
+
−
}
+
−
/* styling for the titles  h3 h4 h5 h6*/
+
−
.igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
+
−
padding:5px 15px;
+
−
border-bottom:0px;
+
−
color: #000000; 
+
−
}
+
−
/* font and text */
+
−
.igem_2017_content_wrapper p {
+
−
padding: 0px 15px;
+
−
font-size: 13px;
+
−
}
+
−
/* Links */
+
−
.igem_2017_content_wrapper a {
+
−
font-weight: bold;
+
−
text-decoration: underline;
+
−
text-decoration-color: #3399ff;
+
−
color:  #3399ff;
+
−
-webkit-transition: all 0.4s ease;
+
−
-moz-transition: all 0.4s ease;
+
−
-ms-transition: all 0.4s ease;
+
−
-o-transition: all 0.4s ease;
+
−
transition: all 0.4s ease;
+
−
}
+
−
/* hover for the links */
+
−
.igem_2017_content_wrapper a:hover {
+
−
text-decoration:none;
+
−
color:#000000;
+
−
}
+
−
/* non numbered lists */
+
−
.igem_2017_content_wrapper ul {
+
−
padding:0px 20px;
+
−
font-size: 13px;
+
−
font-family:Tahoma, Geneva, sans-serif;
+
−
}
+
−
/* numbered lists */
+
−
.igem_2017_content_wrapper ol {
+
−
padding:0px;
+
−
font-size: 13px;
+
−
font-family:Tahoma, Geneva, sans-serif;
+
−
}
+
−
/* Table */
+
−
.igem_2017_content_wrapper table {
+
−
width: 97%;
+
−
margin:15px 10px;
+
−
border: 1px solid #383838;
+
−
border-collapse: collapse;
+
−
}
+
−
/* table cells */
+
−
.igem_2017_content_wrapper  td {
+
−
padding: 10px;
+
−
vertical-align: text-top;
+
−
border: 1px solid #383838;
+
−
border-collapse: collapse;
+
−
}
+
−
/* table headers */
+
−
.igem_2017_content_wrapper th {
+
−
padding: 10px;
+
−
vertical-align: text-top;
+
−
border: 1px solid #383838;
+
−
border-collapse: collapse;
+
−
background-color:#7c6429;
+
−
}
+
−
/**********************************LAYOUT CLASSES **********************************/
+
−
/* general class for column divs */
+
−
.igem_2017_content_wrapper .column  {
+
−
padding: 10px 0px;
+
−
float:left;
+
−
}
+
−
/* class for a full width column */
+
−
.column .full_size {
+
−
width:100%;
+
−
}
+
−
/* styling for images in a full width column*/
+
−
.column.full_size img {
+
−
width:97%;
+
−
padding: 10px 15px;
+
−
}
+
−
/* class for a half width column */
+
−
.column.half_size {
+
−
width: 50%;
+
−
}
+
−
/* styling for images in a half width column*/
+
−
.column.half_size img {
+
−
width: 94.5%;
+
−
padding: 10px 15px;
+
−
}
+
−
/********************************* SUPPORT CLASSES ********************************/
+
−
/* class that clears content below*/
+
−
.igem_2017_content_wrapper .clear {
+
−
clear:both;
+
−
}
+
 
 
−
/* adds extra spacing when clearing content */
+
−
.igem_2017_content_wrapper  .clear.extra_space {
+
<li class="removable-parent">
−
height: 30px;
+
−
}
+
<a class="removable-parent" href="design.html" data-link-type="EXISTING"  target="_self" >
−
/* highlight class, makes content slightly smaller */
+
−
.igem_2017_content_wrapper .highlight {
+
<span id="element-c6287513fca8901"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
−
margin: 0px 15px;
+
</a>
−
padding: 15px 0px;
+
−
}
+
</li>
−
/* highlight class, adds a gray background */
+
−
.igem_2017_content_wrapper .highlight.gray {
+
−
background-color: #7c6429;
+
−
}
+
−
/* highlight with decoration blue line on top */
+
−
.igem_2017_content_wrapper .highlight.blue_top {
+
−
    border-top: 4px solid #3399ff;
+
−
}
+
−
/* highlight with a full blue border decoration */
+
−
.igem_2017_content_wrapper .highlight.blue_border {
+
−
    border: 4px solid  #3399ff;
+
−
}
+
−
/* button class */
+
−
.igem_2017_content_wrapper .button{
+
−
max-width: 35%;
+
−
margin: 30px auto;
+
−
padding: 12px 10px;
+
−
    background-color: #3399ff;
+
−
    text-align: center;
+
−
  color: #ffffff;
+
−
-webkit-transition: all 0.4s ease;
+
−
-moz-transition: all 0.4s ease;
+
−
-ms-transition: all 0.4s ease;
+
−
-o-transition: all 0.4s ease; transition: all 0.4s ease; 
+
−
}
+
−
/* styling for button on hover */
+
−
.igem_2017_content_wrapper .button:hover{
+
−
background-color: #3399ff;
+
−
    color: #000000;
+
−
}
+
−
/***************************************************** RESPONSIVE STYLING ****************************************************/
+
−
/* IF THE SCREEN IS LESS THAN 1200PX */
+
−
@media only screen and (max-width: 1200px) {
+
−
#content {width:100%; }
+
−
.igem_2017_menu_wrapper {width:15%; right:0;}
+
−
.highlight {padding:10px 0px;}
+
−
.igem_2017_menu_wrapper #display_menu_control { display:none; }
+
−
#menu_content { display:block;}
+
−
.menu_button.direct_to_page {padding-left: 17px;}
+
−
}
+
−
/* IF THE SCREEN IS LESS THAN 800PX */
+
−
@media only screen and (max-width: 800px) {
+
−
.igem_2017_menu_wrapper { width:100%; height: 15%; position:relative; left:0%;}
+
−
.igem_2017_content_wrapper {width:100%; margin-left:0px;}
+
−
.column.half_size  {width:100%; }
+
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<p>Cadets2Vets has built a biological arsenic circuit that uses a combination of native DH5-alpha E. coli genes to detect arsenic ions in solution and genetic elements capable of producing GFP (Green Fluorescent Protein). After reading through academic literature, our team found a negative repressor that could be used as an arsenic regulator known as ArsR. Sensitive to arsenic ions, this protein will bind to an operon or promoter sequence and inhibit the transcription of the downstream gene in the presence of a transcription factor (in our case arsenic). Expressing a conformation change, this strategy allows us to control GFP expression using a protein sensitive to and capable of bonding with arsenic ions in solution.</p><p><br></p><p>Integrated DNA Technologies, an iGEM sponsor, provided us with free synthetic DNA to build our circuit. This sponsorship allowed us to select promoters and genes that fit our project. The molecular cloning strategy was to request two GBlocks that would contain a promoter-ribosome binding site-CDS-terminator sequence for constituitively expressing ArsR and regulating the expression of GFP via ArsR. We also added in a restriction enzyme site to both GBlocks that enabled us to combine the two GBlocks together.</p>
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h2 id="vbid-d1ee0827-6dxz4jzx" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >ARS R Regulatory Protein: PcArsR</h2>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<span id="vbid-d1ee0827-mbiql9ax"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<p><strong></strong>BBa_K2248002</p>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<span id="vbid-d1ee0827-pltcwnit"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
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<groupparts>iGEM17 Cadets2Vets</groupparts>
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<groupparts>iGEM17 Cadets2Vets</groupparts>

Latest revision as of 02:20, 1 November 2017

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circuit parts overview





Cadets2Vets has built a biological arsenic circuit that uses a combination of native DH5-alpha E. coli genes to detect arsenic ions in solution and genetic elements capable of producing GFP (Green Fluorescent Protein). After reading through academic literature, our team found a negative repressor that could be used as an arsenic regulator known as ArsR. Sensitive to arsenic ions, this protein will bind to an operon or promoter sequence and inhibit the transcription of the downstream gene in the presence of a transcription factor (in our case arsenic). Expressing a conformation change, this strategy allows us to control GFP expression using a protein sensitive to and capable of bonding with arsenic ions in solution.


Integrated DNA Technologies, an iGEM sponsor, provided us with free synthetic DNA to build our circuit. This sponsorship allowed us to select promoters and genes that fit our project. The molecular cloning strategy was to request two GBlocks that would contain a promoter-ribosome binding site-CDS-terminator sequence for constituitively expressing ArsR and regulating the expression of GFP via ArsR. We also added in a restriction enzyme site to both GBlocks that enabled us to combine the two GBlocks together.


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2304 Jefferson Ave, Tacoma, WA 98402


<groupparts>iGEM17 Cadets2Vets</groupparts>