Difference between revisions of "Team:Cadets2Vets/Parts"

Cadets2Vets has built a biological arsenic circuit that uses a combination of native DH5-alpha E. coli genes to detect arsenic ions in solution and genetic elements capable of producing GFP (Green Fluorescent Protein). After reading through academic literature, our team found a negative repressor that could be used as an arsenic regulator known as ArsR. Sensitive to arsenic ions, this protein will bind to an operon or promoter sequence and inhibit the transcription of the downstream gene in the presence of a transcription factor (in our case arsenic). Expressing a conformation change, this strategy allows us to control GFP expression using a protein sensitive to and capable of bonding with arsenic ions in solution.

Integrated DNA Technologies, an iGEM sponsor, provided us with free synthetic DNA to build our circuit. This sponsorship allowed us to select promoters and genes that fit our project. The molecular cloning strategy was to request two GBlocks that would contain a promoter-ribosome binding site-CDS-terminator sequence for constituitively expressing ArsR and regulating the expression of GFP via ArsR. We also added in a restriction enzyme site to both GBlocks that enabled us to combine the two GBlocks together.

more

<groupparts>iGEM17 Cadets2Vets</groupparts>

Difference between revisions of "Team:Cadets2Vets/Parts"

Latest revision as of 02:20, 1 November 2017

circuit parts overview

Our PArts

SalI Linker Restriction Site

Biosensor: Ars2.0

ARS R Regulatory Protein: PcArsR

GFP: PArsGFP

Follow us on Social Media!

2304 Jefferson Ave, Tacoma, WA 98402

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+			<span id="element-4d7dd9f5c90ef9b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook September</span>
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-<div class="preview-subtitle-holder removable-parent order-handle">
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-		<p style="text-align: center;">- 2017 iGEM Team -</p>
+		<p>Cadets2Vets has built a biological arsenic circuit that uses a combination of native DH5-alpha E. coli genes to detect arsenic ions in solution and genetic elements capable of producing GFP (Green Fluorescent Protein). After reading through academic literature, our team found a negative repressor that could be used as an arsenic regulator known as ArsR. Sensitive to arsenic ions, this protein will bind to an operon or promoter sequence and inhibit the transcription of the downstream gene in the presence of a transcription factor (in our case arsenic). Expressing a conformation change, this strategy allows us to control GFP expression using a protein sensitive to and capable of bonding with arsenic ions in solution.</p><p><br></p><p>Integrated DNA Technologies, an iGEM sponsor, provided us with free synthetic DNA to build our circuit. This sponsorship allowed us to select promoters and genes that fit our project. The molecular cloning strategy was to request two GBlocks that would contain a promoter-ribosome binding site-CDS-terminator sequence for constituitively expressing ArsR and regulating the expression of GFP via ArsR. We also added in a restriction enzyme site to both GBlocks that enabled us to combine the two GBlocks together.</p>
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-</div>
-<br class="lower-line-break" />
-												<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
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@@ Line 496: / Line 661: @@
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+<div class="layout-settings" style="display:none;" data-type="blocks"></div>
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 <div  class="multi-container preview image-cover" >
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-			<div class="helper-div middle-left" >
+			<div class="helper-div middle-right" >
 			<!-- <div class="benet" style="min-height:inherit;"></div> -->
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-					<div id="vbid-59a5b-2w1tabau-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
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+	<a class="image-link top-layer not-wrapping" href="http://parts.igem.org/Part:BBa_K2248000"  data-link-type="EXTERNAL" target="_blank" ></a>
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@@ Line 554: / Line 721: @@
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+										<!--  BY NATIVE ORDER -->
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-	<div class="preview-icon-holder Icon removable-parent order-handle" >
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+		<a href="http://parts.igem.org/Part:BBa_K2248000"  data-link-type="EXTERNAL" target="_blank" >
+	<h2 id="vbid-d1ee0827-ldivvjwr" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >SalI Linker Restriction Site</h2>
+	</a>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
+<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 <br class="upper-line-break" />
-<div class="preview-subtitle-holder removable-parent order-handle">
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+		<a href="http://parts.igem.org/Part:BBa_K2248000"  data-link-type="EXTERNAL" target="_blank" >
+	<div id="vbid-d1ee0827-usb41req" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+		<p>BBa_K2248000</p>
+	</div>
+	</a>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
+											<div class="preview-form order-handle">
+<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
+</div>
+											<div class="preview-item-links order-handle ">
-												<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
+	<div class="preview-links-wrapper">
-	<span id="vbid-59a5b-hapd8kb8"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
+		<a class="removable-parent" href="http://parts.igem.org/Part:BBa_K2248000" data-link-type="EXTERNAL"   target="_blank" >
+		<span id="vbid-d1ee0827-e7htftnd"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
+			</a>
+<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
+	</div>
 </div>
+<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
+<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
@@ Line 631: / Line 819: @@
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+					<div class="item-content leaf multi_layout page content -container" data-self="vbid-e22d8bff-opvd7oao" data-preview-style="style-d1ee0827-r7hdefvx" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="525" data-orig-thumb-width="544" data-vbid="vbid-e22d8bff-opvd7oao" data-bgimg="https://static.igem.org/mediawiki/2017/b/ba/C2V_Parts_BiosensorArs2.png">
 <div  class="multi-container preview image-cover" >
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 			</div>
-			<div class="helper-div middle-left" >
+			<div class="helper-div middle-right" >
 			<!-- <div class="benet" style="min-height:inherit;"></div> -->
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 				<div class="vertical-aligner">
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+					<div id="vbid-e22d8bff-wths8jko-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+	<a class="image-link top-layer not-wrapping" href="http://parts.igem.org/Part:BBa_K2248003"  data-link-type="EXTERNAL" target="_blank" ></a>
-	<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
+	<div  id="vbid-e22d8bff-wths8jko" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/b/ba/C2V_Parts_BiosensorArs2.png);"  data-orig-width="544" data-orig-height="525" >
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@@ Line 681: / Line 865: @@
 							<div class="preview-content-holder shrinker-content">
-									<!--  BY SPECIFIC ORDER -->
+										<!--  BY NATIVE ORDER -->
+<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
-	<div class="preview-icon-holder Icon removable-parent order-handle" >
-		<img id="vbid-59a5b-fhlvsspa" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://lh3.googleusercontent.com/RV9GQfQJ9BfAg_aSqdCOkSsuOMs7KUIGWbLAgwgxWEQsO3uW297q-3ex471NB0-4m-af1nKLiwPpEUHf" />
-	</div>
 <br class="upper-line-break" />
 <div class="preview-title-holder removable-parent order-handle">
-	<h2 id="vbid-59a5b-6gqtswss" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >WE ARE Cadets2Vets.</h2>
+		<a href="http://parts.igem.org/Part:BBa_K2248003"  data-link-type="EXTERNAL" target="_blank" >
+	<h2 id="vbid-e22d8bff-au235fzu" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Biosensor: Ars2.0</h2>
+	</a>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
+<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 <br class="upper-line-break" />
-<div class="preview-subtitle-holder removable-parent order-handle">
+<div class="preview-body-holder removable-parent order-handle">
-	<h3 id="vbid-59a5b-uaxcvtbo" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, <br>Tacoma, WA 98402<br></h3>
+		<a href="http://parts.igem.org/Part:BBa_K2248003"  data-link-type="EXTERNAL" target="_blank" >
+	<div id="vbid-e22d8bff-4cfbkvar" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+		<p>BBa_K2248003</p>
+	</div>
+	</a>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
+											<div class="preview-form order-handle">
+<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
+</div>
+											<div class="preview-item-links order-handle ">
-												<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
+	<div class="preview-links-wrapper">
-	<span id="vbid-59a5b-ji7uyawu"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
+		<a class="removable-parent" href="http://parts.igem.org/Part:BBa_K2248003" data-link-type="EXTERNAL"   target="_blank" >
+		<span id="vbid-e22d8bff-ub3mbr5k"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
+			</a>
+<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
+	</div>
 </div>
+<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
+<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
@@ Line 758: / Line 963: @@
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 			<div class="page-wrapper item-wrapper ">
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+					<div class="item-content leaf multi_layout page content -container" data-self="vbid-d1ee0827-s5uasg51" data-preview-style="style-d1ee0827-r7hdefvx" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="230" data-orig-thumb-width="317" data-vbid="vbid-d1ee0827-s5uasg51" data-bgimg="https://static.igem.org/mediawiki/2017/1/11/C2V_Parts_ARSRRegulatoryProteinPcArsR.gif">
 <div  class="multi-container preview image-cover" >
 	<div class="Picture item-preview">
@@ Line 768: / Line 973: @@
 			<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 			</div>
-			<div class="helper-div middle-left" >
+			<div class="helper-div middle-right" >
 			<!-- <div class="benet" style="min-height:inherit;"></div> -->
 			<div class="pic-side">
 				<div class="vertical-aligner">
-					<div id="vbid-59a5b-5afcoeuc-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+					<div id="vbid-d1ee0827-gajgul6y-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
-	<div  id="vbid-59a5b-5afcoeuc" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/R9AFGXNYLfVGedqYcXt8wZoBUZa-2LuG0k9dhpPFpXAmOTcmWUe9SxiUjF40LhTsept7tdAu2fxW35vYxw=s300);"  data-orig-width="768" data-orig-height="512" >
+	<a class="image-link top-layer not-wrapping" href="http://parts.igem.org/Part:BBa_K2248001"  data-link-type="EXTERNAL" target="_blank" ></a>
+	<div  id="vbid-d1ee0827-gajgul6y" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/1/11/C2V_Parts_ARSRRegulatoryProteinPcArsR.gif);"  data-orig-width="317" data-orig-height="230" >
 		<!-- VIDEO TEMPLATE -->
@@ Line 802: / Line 1,009: @@
 							<div class="preview-content-holder shrinker-content">
-									<!--  BY SPECIFIC ORDER -->
+										<!--  BY NATIVE ORDER -->
+<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
-	<div class="preview-icon-holder Icon removable-parent order-handle" >
-		<img id="vbid-59a5b-ajub3fyf" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://lh3.googleusercontent.com/RV9GQfQJ9BfAg_aSqdCOkSsuOMs7KUIGWbLAgwgxWEQsO3uW297q-3ex471NB0-4m-af1nKLiwPpEUHf" />
-	</div>
 <br class="upper-line-break" />
 <div class="preview-title-holder removable-parent order-handle">
-	<h2 id="vbid-59a5b-doe6dbuz" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >WE ARE Cadets2Vets.</h2>
+		<a href="http://parts.igem.org/Part:BBa_K2248001"  data-link-type="EXTERNAL" target="_blank" >
+	<h2 id="vbid-d1ee0827-6dxz4jzx" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >ARS R Regulatory Protein: PcArsR</h2>
+	</a>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
+<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 <br class="upper-line-break" />
-<div class="preview-subtitle-holder removable-parent order-handle">
+<div class="preview-body-holder removable-parent order-handle">
-	<h3 id="vbid-59a5b-fzo4hggo" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, <br>Tacoma, WA 98402<br></h3>
+		<a href="http://parts.igem.org/Part:BBa_K2248001"  data-link-type="EXTERNAL" target="_blank" >
+	<div id="vbid-d1ee0827-bsa85a5k" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+		<p>BBa_K2248001</p>
+	</div>
+	</a>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
+											<div class="preview-form order-handle">
+<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
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+											<div class="preview-item-links order-handle ">
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+		<span id="vbid-d1ee0827-mbiql9ax"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >more</span>
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@@ Line 876: / Line 1,104: @@
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+	<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lightbox?action=raw&ctype=text/javascript"></script>
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-<div  class="login-dialog-wrapper white_content delay-anim" style="text-align:center;">
-	<div class="login dialog-holder">
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-			<input id="login-btn" class="clickable" type="button" class="input-btn" value="LOGIN">
-			<div id="goto-register" class="clickable t-t">Create a new account</div>
-			<div id="forgot-password" class="clickable t-t">Forgot Password?</div>
-		</form>
-	</div>
-	<div class="register dialog-holder" style="display:none;">
-		<div  class="login-title"><span class="im-text form-type  t-t">Login</span></div>
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-			<input name="password" type="password"  placeholder="password" data-placeholder="password" autocomplete="off" /><br/>
-			<input id="terms" type="checkbox" checked="checked" disabled /><span class="form-label"><a href="http://www.imcreator.com/terms-of-service" target="_blank" class="terms-link  t-t">Terms and Conditions</a></span><br/>
-			<input id="register-btn" class="clickable" type="button" class="input-btn" value="JOIN">
-			<div id="goto-login" class="clickable t-t">Already a member?</div>
-		</form>
-	</div>
-	<div class="forgot-pass dialog-holder" style="display:none;">
-		<div  class="login-title"><span class="im-text form-type t-t">Login</span></div>
-		<form id="forgot-pass-form">
-			<img id="user-gravatar" src="https://www.gravatar.com/avatar/?d=mm" /><br/>
-			<input name="email" type="text" placeholder="email" data-placeholder="email" /><br/>
-			<input id="forgot-pass-btn" class="clickable" type="button" class="input-btn" value="SEND">
-			<div id="goto-login" class="clickable t-t">Back</div>
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-</div>
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-	<div id="fade" class="black_overlay fadein-noscript" style="display:none;"></div>
 </html>
-<---
-<html>
-<div class="column full_size">
-<h1>Parts</h1>
-<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
-<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
-</div>
-<div class="column half_size">
-<div class="highlight">
-<h5>Note</h5>
-<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
-</div>
-</div>
-<div class="column half_size">
-<h5>Adding parts to the registry</h5>
-<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
-<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
-</div>
-<div class="column half_size">
-<h5>What information do I need to start putting my parts on the Registry?</h5>
-<p>The information needed to initially create a part on the Registry is:</p>
-<ul>
-<li>Part Name</li>
-<li>Part type</li>
-<li>Creator</li>
-<li>Sequence</li>
-<li>Short Description (60 characters on what the DNA does)</li>
-<li>Long Description (Longer description of what the DNA does)</li>
-<li>Design considerations</li>
-</ul>
-<p>
-We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
-</div>
-<div class="column half_size">
-<h5>Inspiration</h5>
-<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
-<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
-<ul>
-<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
-<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
-<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
-</ul>
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-<h5>Part Table </h5>
-<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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 <groupparts>iGEM17 Cadets2Vets</groupparts>
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