Difference between revisions of "Team:HBUT-China"

 
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       <ul>
 
       <ul>
 
         <li><img src="https://static.igem.org/mediawiki/2017/5/55/HBUT-logo2.png" height="45"/></li>
 
         <li><img src="https://static.igem.org/mediawiki/2017/5/55/HBUT-logo2.png" height="45"/></li>
        <li><a href='https://2017.igem.org/Team:HBUT-China'>HOME</a></li>
 
        <li class='has-sub'><a href='#'>TEAM</a>
 
            <ul>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Team'>Team</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Collaborations'>Collaborations</a></li>
 
            </ul>
 
        </li>
 
        <li class='has-sub'><a href='javascript:;'>PROJECT</a>
 
            <ul>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Description'>Description</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Design'>Design</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Experiments'>Experiments</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Notebook'>Notebook</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/InterLab'>InterLab</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Model'>Model</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Results'>Results</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Demonstrate'>Demonstrate</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Attributions'>Attributions</a></li>
 
            </ul>
 
        </li>
 
        <li class='has-sub'><a href='javascript:;'>PARTS</a>
 
            <ul>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Basic_Parts'>Basic Parts</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Composite_Parts'>Composite Parts</a></li>
 
            </ul>
 
        </li>
 
        <li><a href='https://2017.igem.org/Team:HBUT-China/SAFETY'>SAFETY</a></li>
 
        <li class='has-sub'><a href='javascript:;'>HUMAN PRACTICES </a>
 
            <ul>
 
                <li ><a href='https://2017.igem.org/Team:HBUT-China/HP/Silver'>Silver HP</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/HP/Gold_Integrated'>Integrated and Gold</a></li>
 
              <li ><a href='https://2017.igem.org/Team:HBUT-China/Engagement'>Public Engagement</a></li>
 
            </ul>
 
        </li>
 
 
       </ul>
 
       </ul>
 
   </div>
 
   </div>
 
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        <a href="javascript:;"><img src="https://static.igem.org/mediawiki/2017/5/55/HBUT-logo2.png" width="300" class="wp1_3 slide1_bot"/></a>               
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        <span class="txt1">Nickel ion!</span>       
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<img src="//2017.igem.org/wiki/images/8/8c/Hbut-banner1.png" alt="">
        <span class="txt2">Heavy metal pollutants!</span>                                 
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        <span class="txt3 short">For this year¡¯s iGEM Competition, our team will be focusing on putting forward a biological approach to detect nickel ions as a heavy metal contaminant in the natural environment</span>                                   
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        <span class="txt1 blue txt_right2">In order to achieve this goal, we use a nickel-sensitive repressive promoter (pncrA)</span>      
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        <span class="txt1 blue txt_right2">In the absence of nickel ions, the repressor protein (NcrB) binds to the promoter, preventing the expression of the subsequent gene</span>
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             </div>
 
             </div>
 
             <small>Overview</small>
 
             <small>Overview</small>
             <p>For this year's iGEM Competition, our team will be focusing on putting forward a biological approach to detect nickel ions as a heavy metal contaminant in the natural environment. Through our study over this summer, we are expecting to make the expression of red fluorescent protein by nickel ion concentration control. In a certain concentration range, we will try to establish a quantitative relationship between fluorescence intensity and nickel ion concentration in the environment. This may build a nickel ion concentration quantitative detection system.</p>
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             <p>For this year's iGEM Competition, our team has focused on putting forward a biological approach to detecting nickel ions as a heavy metal contaminant in the natural environment. Through our study over this summer, we are expecting to control the expression of red fluorescent protein by changing the nickel ion concentration. In a certain concentration range, we will try to establish a quantitative relationship between the intensity of the fluorescence and the concentration of nickel ions in the environment. This may build a nickel ion concentration quantitative detection system.</p>
 
             <p>In order to achieve this goal, we use a nickel-sensitive repressive promoter (pncrA). In the absence of nickel ions, the repressor protein (NcrB) binds to the promoter, preventing the expression of the subsequent gene. The nickel ions in the environment can bind to repressor proteins to alter the domain of the protein, so that the fluorescent protein gene behind the promoter is expressed and produces fluorescence.</p>
 
             <p>In order to achieve this goal, we use a nickel-sensitive repressive promoter (pncrA). In the absence of nickel ions, the repressor protein (NcrB) binds to the promoter, preventing the expression of the subsequent gene. The nickel ions in the environment can bind to repressor proteins to alter the domain of the protein, so that the fluorescent protein gene behind the promoter is expressed and produces fluorescence.</p>
 
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   <p>copyright &copy;  2017 IGEM-HBUT-China All rights reserved.</p>
 
   <p>copyright &copy;  2017 IGEM-HBUT-China All rights reserved.</p>
 
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Latest revision as of 02:33, 1 November 2017

Overview

For this year's iGEM Competition, our team has focused on putting forward a biological approach to detecting nickel ions as a heavy metal contaminant in the natural environment. Through our study over this summer, we are expecting to control the expression of red fluorescent protein by changing the nickel ion concentration. In a certain concentration range, we will try to establish a quantitative relationship between the intensity of the fluorescence and the concentration of nickel ions in the environment. This may build a nickel ion concentration quantitative detection system.

In order to achieve this goal, we use a nickel-sensitive repressive promoter (pncrA). In the absence of nickel ions, the repressor protein (NcrB) binds to the promoter, preventing the expression of the subsequent gene. The nickel ions in the environment can bind to repressor proteins to alter the domain of the protein, so that the fluorescent protein gene behind the promoter is expressed and produces fluorescence.