Difference between revisions of "Team:UNC-Asheville/Attributions"

 
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<button class="btn btn-primary btn-block text-left" type="button" data-toggle="collapse" data-target="#Wetwork" aria-expanded="false" aria-controls="Wetwork">
     <h2>Agar Plate Pouring</h2>
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     <blockquote>
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     <h2>Nicholas White was responsible for overall project design and implementation. He designed the plasmids, created experiment protocols, and performed the bulk of the labwork.</h2>
Autoclave<br>
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<br>
Water bath<br>
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<h2>Katie Brown performed wetlab protocols pertaining to bacterial cultures, restriction, ligation and experiment visualization and confirmation.</h2>
Pipetman<br>
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<br>
- 37 g pre-mixed powder consisting of:<br>
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<h2>William Jackson performed tasks related to sample prep, PCR, lab management, creation and repair of lab equipment.</h2>
5 g peptone<br>
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10.0 g peptone from casein<br>
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10.0 g sodium chloride<br>
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12.0 g agar-agar<br>
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1 L Sterile H2O<br>
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Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10 mL of agar and on which you can individually distinguish a maximum of ~100 colonies
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*Pro-Tip* If you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of agar.
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Autoclavable flasks
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Sterile pipettes
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    <h2>Nicholas White was responsible for plasmid design, protocol design, and experiment implementation.</h2>
Ice bucket to hold antibiotic
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<br>
Antibiotic at 1000 x concentration disolved in the appropriate liquid solvent. See table to the right for appropriate antibiotic concentrations.
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    <h2>William Jackson was responsible for the wiki, implementation of the model and iGEM deliverables.</h2>
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<h2>Jonathon Deutsch was responsible for mathematical representation of the model and research.</h2>
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    <h2>Human Practices</h2>
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<h2>Katie Brown was responsible for Human Practices Programming and Content.</h2>
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<br>
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     <h2>William Jackson was responsible for arranging logistics for Human Practices .</h2>
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Latest revision as of 02:43, 1 November 2017

About Us

iGEM UNC-Asheville Attributions

Attributions

Let's find out who we need to yell at.




A Disclaimer
In comparison to most schools, this list will look sparse. Our team was composed of four motivated people, and was driven by and for students. While we received advice from many, our triumphs and failures lie squarely on our shoulders.



Nicholas White was responsible for overall project design and implementation. He designed the plasmids, created experiment protocols, and performed the bulk of the labwork.


Katie Brown performed wetlab protocols pertaining to bacterial cultures, restriction, ligation and experiment visualization and confirmation.


William Jackson performed tasks related to sample prep, PCR, lab management, creation and repair of lab equipment.

Nicholas White was responsible for plasmid design, protocol design, and experiment implementation.


William Jackson was responsible for the wiki, implementation of the model and iGEM deliverables.


Jonathon Deutsch was responsible for mathematical representation of the model and research.


Katie Brown was responsible for Human Practices Programming and Content.


William Jackson was responsible for arranging logistics for Human Practices .