Difference between revisions of "Team:Cadets2Vets/Design"

 
(22 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{Cadets2Vets}}
+
{{Team:Cadets2Vets/SideMenu}}
 +
 
 
<html>
 
<html>
 +
<head>
 +
<title>
 +
Design - Home</title>
 +
<!-- META -->
 +
<meta charset="UTF-8">
 +
<meta name="viewport" content="width=device-width, maximum-scale=1"/>
 +
 +
 +
 +
 +
<link href="https://2017.igem.org/Team:Cadets2Vets/css/common?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/all_css?action=raw&ctype=text/css" rel="stylesheet">
 +
<link rel="icon" type="image/png" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
 +
<link rel="apple-touch-icon" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
 +
        <link href="https://2017.igem.org/Team:Cadets2Vets/css/design/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
 +
<link href="https://2017.igem.org/Team:Cadets2Vets/css/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
 +
        <link href="https://2017.igem.org/Team:Cadets2Vets/css/fonts?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/effects?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/lightbox?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/spimeview?action=raw&ctype=text/css" rel="stylesheet">
 +
        <link href="https://2017.igem.org/Team:Cadets2Vets/css/design/static_style?action=raw&ctype=text/css" rel="stylesheet">
 +
<!-- SCRIPT -->
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/xprs_helper?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/all_js?action=raw&ctype=text/javascript"></script>
 +
                <script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lib/touchswipe/jquery_mobile_custom_min?action=raw&ctype=text/javascript"></script>
 +
 +
 +
 +
 +
 +
<!-- User analytics -->
  
−
<head><meta name="viewport" content="width=device-width, initial-scale=1"><meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><script>function disputeIframe(){setTimeout(function (){self.document.open();self.document.write("<h1><a style='font-weight: normal; color: #000; font-family: arial;' href='" + self.location.href + "' target='_top'>Iframes are not supported on a free plan.</a></h1>");                    self.document.close();},200);} try {if (self!=top){disputeIframe();}}catch(e){disputeIframe();}</script><link href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/bootstrap3.min.css" rel="stylesheet"><link rel="stylesheet" href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/font-awesome.min4.css"><link href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/hover-min.css" rel="stylesheet"><link href="/static/css/style.css?v=2.0.4" rel="stylesheet"><link href="/static/css/blocks.css?v=2.0.4" rel="stylesheet"><link href="/static/css/theme-css/ez.css?v=2.0.4" rel="stylesheet"><style  type='text/css' data='Page Custom CSS'></style><script src="//code.jquery.com/jquery-1.12.4.min.js" integrity="sha256-ZosEbRLbNQzLpnKIkEdrPv7lOy9C27hHQ+Xp8a4MxAQ=" crossorigin="anonymous"></script><script> if (!window.jQuery) {  document.write('<script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/jquery.js"></scr' + 'ipt>'); }</script><meta name="page-source" site="//sites.simbla.com/52b5a023-1bcf-d512-a412-3351ef830f04" page="design" site-id="593aca5737cad4ed7fa1fc16" page-id="59416e8195f86d9166bcfe25" master-page-id="" /><link rel="canonical" href="//sites.simbla.com/52b5a023-1bcf-d512-a412-3351ef830f04/design" /></head><body data-spy="scroll" data-target=".navbar-collapse" class="static-page"><div id="windowDiv"><div id="MarginsTop" style="height: 0px;" class="row"></div><div id="bodyContainer" style="width:100%"><div class="simblaEL simblaBlock headerBlock containerHolder" data-drag="P0" id="P0" style="position: relative;"><a class="simblaEL anchorElm" data-drag="divCounter" name="Intro0" id="Intro0"></a><div class="container" style="width: 100%; padding: 0px;"><header class="simblaEL container-fluid" data-drag="P2" id="P2"><div class="row simblaEL rDivider" data-drag="P4" id="P4" style="position: relative;"><div class="sDivider col-md-2 index0" data-colsize="2"></div><div class="sDivider col-md-10 index1" data-colsize="10"></div></div></header></div></div><div class="simblaEL containerHolder" data-drag="P0" id="P0" style=""><a class="simblaEL anchorElm" data-drag="divCounter" name="Intro0" id="Intro0"></a><div class="container-fluid minHeight60 active" data-border-type="All" style="overflow: hidden; min-height: 416.4px; border-radius: 0px; background-image: url(&quot;//d33rxv6e3thba6.cloudfront.net/2015/10/5648949c3a571f390c74a1cb/3153-1yh8305.png&quot;); background-attachment: initial; background-color: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: repeat;"><div class="simblaEL containerHolder" data-drag="P10" id="P10" style="position: relative;"><div class="container" style=""><div class="row simblaEL rDivider" data-drag="P12" id="P12" style="position: relative; padding-top: 30px;"><div class="sDivider col-md-6 index0" data-colsize="6"><div class="simblaEL tcH" data-drag="P14" id="P14" style="position: relative;"><div class="textContainer H1" style="cursor: text; padding-top: 80px;"><h1>Design</h1></div></div><div class="simblaEL tcH" data-drag="P16" id="P16"><div class="textContainer H2" style="cursor: text;"><h2><div style="text-align: left;">The foundation of our circuit</div></h2></div></div><div class="simblaEL tcH" data-drag="P18" id="P18" style="position: relative;"><div class="textContainer H3" style="cursor: text; padding-bottom: 30px; padding-right: 20px;"><h3><font style="font-size: 24px;" color="#444444">
 
−
Our circuit was designed to utilize specific parts so that when the E-coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.&nbsp;</font><div><br></div></h3></div></div></div><div class="sDivider col-md-6 index1" data-colsize="6"><div class="simblaEL simblaImg" data-drag="P20" id="P20"><img draggable="false" class="" alt="" data-id="" title="" src="https://s3.amazonaws.com/simbla-static/2017/5/593aca5737cad4ed7fa1fc16/12707-ymd99p.jpg"></div></div></div></div></div></div></div><div class="simblaEL containerHolder" data-drag="P113" id="P113" style="position: relative;"><a class="simblaEL anchorElm" data-drag="divCounter" name="Content1" id="Content1" data-id="5941796e95f86d9166bcfe6c"></a><div class="container-fluid" style="border-radius: 0px; padding-top: 50px; padding-bottom: 50px;" data-border-type="All"><div class="row simblaEL rDivider" data-drag="P115" id="P115" style="position: relative;"><div class="sDivider col-md-4 index0" data-colsize="4"><div class="simblaEL simblaGallery" data-drag="P117" id="P117" data-g-hover-color="" data-g-text-color="" data-g-style="FADE" data-g-spacing="NORMAL"><div class="row simblaEL rDivider"><div class="sDivider elem col-md-12 col-sm-12"><figure class="simblaEL simblaImg" data-drag="P119" id="P119" style="border-radius: 0px;"><img draggable="false" class="" style="border-radius: 0px;" alt="" data-id="" title="" src="http://synbio.info/download/attachments/3867064/engineering.jpg?version=1&amp;modificationDate=1455211151162&amp;api=v2"width="500" height="500"><figcaption></figcaption></figure></div></div></div><div class="simblaEL tcH" data-drag="P121" id="P121" style="position: relative;"><div class="textContainer H2" style="margin-left: 10px; cursor: text;"><h2>PHASE 1</h2></div></div><div class="simblaEL tc" data-drag="P123" id="P123" style="position: relative;"><div class="textContainer" style="cursor: text; margin-left: 10px; line-height: 24px;"><p style="line-height: 24px;"></p><ul><li><font style="font-size: 18px;">Test efficacy of the circuit in vivo&nbsp;<br></font></li><li><font style="font-size: 18px;">Grow transformed e.coli with plasma circuit in Arsenic laced media &nbsp;&nbsp;</font></li><li><font style="font-size: 18px;">Serial dilution of arsenic and arsenic to find the lowest activation amount of the circuit in vivo</font><br></li></ul><p></p></div></div></div><div class="sDivider col-md-4 index1" data-colsize="4"><div class="simblaEL simblaGallery" data-drag="P127" id="P127" data-g-hover-color="" data-g-text-color="" data-g-style="FADE" data-g-spacing="NORMAL"><div class="row simblaEL rDivider"><div class="sDivider elem col-md-12 col-sm-12"><figure class="simblaEL simblaImg" data-drag="P129" id="P129" style="border-radius: 0px;"><img draggable="false" class="" style="border-radius: 0px;" alt="" data-id="" title="" src="https://media.licdn.com/mpr/mpr/AAEAAQAAAAAAAAP0AAAAJDJhNzEwNzE0LTBmNjQtNGViOS04OTFlLTJjODI1MjEwNDExYw.jpg"width="500" height="500"><figcaption></figcaption></figure></div></div></div><div class="simblaEL tcH" data-drag="P131" id="P131" style="position: relative;"><div class="textContainer H2" style="margin-left: 10px; cursor: text;"><h2>PHASE 2</h2></div></div><div class="simblaEL tc" data-drag="P133" id="P133" style="position: relative;"><div class="textContainer" style="cursor: text; margin-left: 10px; line-height: 24px;"><p style="line-height: 24px;"></p><ul><li><font style="font-size: 18px;">Test the efficacy of the plasma circuit in S.I.T.U. &nbsp;&nbsp;<br></font></li><li><font style="font-size: 18px;">Perform above experiments with prepared plasma circuit &nbsp;&nbsp;<br></font></li><li><font style="font-size: 18px;">Titrate both circuit and trigger to find optimal mixture &nbsp;&nbsp;<br></font></li><li><font style="font-size: 18px;">Standardize reactions &nbsp; &nbsp;<br></font></li><li><font style="font-size: 18px;">Test on paper matrix</font><br></li></ul><p></p></div></div></div><div class="sDivider col-md-4 index2" data-colsize="4"><div class="simblaEL simblaGallery" data-drag="P137" id="P137" data-g-hover-color="" data-g-text-color="" data-g-style="FADE" data-g-spacing="NORMAL"><div class="row simblaEL rDivider"><div class="sDivider elem col-md-12 col-sm-12"><figure class="simblaEL simblaImg" data-drag="P139" id="P139" style="border-radius: 0px;"><img draggable="false" class="" style="border-radius: 0px;" alt="" data-id="" title="" src="https://static.igem.org/mediawiki/2016/b/b2/T--SCAU-China--logo_2.png"><figcaption></figcaption></figure></div></div></div><div class="simblaEL tcH" data-drag="P141" id="P141" style="position: relative;"><div class="textContainer H2" style="margin-left: 10px; cursor: text;"><h2>PHASE 3</h2></div></div><div class="simblaEL tc" data-drag="P143" id="P143" style="position: relative;"><div class="textContainer" style="cursor: text; margin-left: 10px; line-height: 24px;"><p style="line-height: 24px;"></p><ul><li><font style="font-size: 18px;">Test efficacy of freeze drying circuit on paper for distribution and storage purposes. &nbsp;</font> &nbsp;<br></li></ul><p></p><p style="line-height: 24px;"><br></p></div></div></div></div></div></div></div></div><a href="https://www.simbla.com" target="_blank" id="linkToSimbla" class="en"><span class="main">SIMBLA</span><span class="s">Website Builder</span></a><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/jquery.bootpag.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/bootstrap3.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/Chart.min.js"></script><script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/numeral.min.js"></script><script src="/static/js/simbla.js?v=2.0.4"></script><script src="https://www.google.com/recaptcha/api.js?onload=onloadRecaptcha&render=explicit" async defer></script></body>
 
  
 +
<!-- Serving analytics -->
 +
<script>
 +
var currentDomain =  window.location.host;
 +
if (currentDomain != "localhost:7000"){
 +
var _gaq = _gaq || [];
 +
_gaq.push(['_setAccount', 'UA-58695071-1']);
 +
_gaq.push(['_setDomainName', currentDomain]);
 +
_gaq.push(['_setAllowLinker', true]);
 +
_gaq.push(['_trackPageview']);
 +
}
 +
</script>
 +
 +
<!-- Label config-->
 +
<script>
 +
 +
var LABEL_CONFIG = {};
 +
 +
</script></head>
  
 +
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.1f" data-caller="live"  data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 +
 +
<div id="xprs" data-website-name="Home" class="xprs-holder" >
 +
<div class="main-page "  >
 +
<div id="content">
 +
<div  id="vbid-59a5b-kmybr6mb"  class="master container style-59a5b-k04kaozm content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Design"  data-itemslug="design" data-itemstyleid="style-59a5b-k04kaozm" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-59a5b-kmybr6mb" data-preset-type-id="ITEMS" data-preview-style="style-59a5b-k04kaozm" data-style="style-5a24e-kzdyurm3ju" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="stripes"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="middle"></div>
 +
<div id="children">
 +
 +
 +
 +
<!-- MENUS START -->
 +
<div  id="vbid-59a5b-1xbnq2ek"  class="master item-box  header-box style-59a5b-jbqq3wgo injected        " data-holder-type="header"" data-holder-type="header"  data-child-type="ORIGIN"  data-styleid="style-59a5b-jbqq3wgo" data-preview-styleid='style-59a5b-jbqq3wgo' data-preset-type-id="MENUS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="header-wrapper item-wrapper menus-wrapper ">
 +
 +
<div class="item-content leaf menu_layout header content" data-preview-style="style-59a5b-jbqq3wgo" data-style="style-7f715-scjjarqgxu"  data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-1xbnq2ek" data-bgimg="">
 +
<div class="preview-content-wrapper">
 +
<div class="preview-content-holder">
 +
<div class="left-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="logo-holder">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
  
 +
</div>
 +
<div class="helper-div">
 +
<div class="item-details menu">
 +
<!-- TITLE TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
  
 +
<!-- SUBTITLE TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
  
 +
</div>
 +
</div>
 +
</div>
 +
<div class="right-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<!-- LINKS TEMPLATE -->
 +
<div class="preview-item-links ">
 +
<ul class="preview-links-wrapper">
  
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="index.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-a1a56421418f7ce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="collaborations.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-5efad87d43e4352"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="description.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-e6e2afb357139e4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Description</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="design.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-c6287513fca8901"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="experiments.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-f4b485a3e8b3825"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-603d2303312c4bd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-august.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-e39fe0beffa33cd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook August</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-july.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-09ca318ecaf1b2f"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook July</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-june.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-367041e6445b247"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook June</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="public-engagement.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-307cb61a9a844e1"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="safety.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-fa38138e1dd4fce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="team.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-8308751370782bc"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="basic-parts.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-78fbd0423085ce2"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="composite-parts.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-24ea54f35798e9a"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Composite Parts</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="parts.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-8d68d4dbac38a10"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="interlab.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-c92600692c09125"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Interlab</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="our-partners.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-be35f8069be1925"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Our Partners</span>
 +
</a>
 +
 +
</li>
  
−
<div class="column full_size">
+
−
<h1>Design</h1>
+
−
<p>
+
<li class="removable-parent">
−
Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
+
−
</p>
+
<a class="removable-parent" href="notebook-september.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-4d7dd9f5c90ef9b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook September</span>
 +
</a>
 +
 +
</li>
  
−
<p>
+
−
This page is different to the "Applied Design Award" page. Please see the <a href="https://2017.igem.org/Team:Cadets2Vets/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
+
−
</p>
+
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="environmental-project.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="2d522622"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Environmental Project</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="hardware.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="8d3cfa8c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Hardware</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="demonstrate.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="improve.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="results.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="7e0ed437"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Results</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="attribution.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="8a69633b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Attribution</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-october.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="643d5085"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="silver-hp.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-563894f97a8ebaf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Silver HP</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="integrated-and-gold.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-874635e92416b44"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</ul>
 +
</div>
 +
 +
 +
<button class="hamburger links-menu-btn  small" type="button">
 +
  <span class="hamburger-box">
 +
  <span class="hamburger-inner"></span>
 +
  </span>
 +
</button>
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="menu"
 +
data-MENU_OVERLAY="relative"
 +
data-MENU_SCROLL="false"
 +
data-ALWAYS_MINIFIED="false"
 +
data-MENU_POSITION="none"
 +
data-MENU_ALIGN="left"
 +
data-BACKGROUND_COLOR="rgb(124, 100, 41)"
 +
></div>
 
</div>
 
</div>
 +
 +
</div>
 +
</div>
 +
<!-- MENUS END -->
 +
 +
 +
 +
 +
<!-- PROMO START -->
 +
<div  id="vbid-59a5b-kkrzqoyd"  class="master item-box  gallery-box style-59a5b-wopgoz9q      items-enter-effects item_enter3 fill-height  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-wopgoz9q" data-preview-styleid='style-59a5b-wopgoz9q' data-preset-type-id="PROMO">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 +
 +
<div  class="sub container style-59a5b-wopgoz9q content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-59a5b-wopgoz9q" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-59a5b-kkrzqoyd" data-preset-type-id="PROMO" data-preview-style="style-59a5b-wopgoz9q" data-style="style-89a8e-bbtqrmxrkr" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-59a5b-a9iahv3h"  class="sub item-box  page-box style-59a5b-wopgoz9q          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-wopgoz9q" data-preview-styleid='style-59a5b-wopgoz9q' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-a9iahv3h" data-preview-style="style-59a5b-wopgoz9q" data-style="style-89a8e-q4q4gr5nhr" data-orig-thumb-height="2304" data-orig-thumb-width="3456" data-vbid="vbid-59a5b-a9iahv3h" data-bgimg="https://static.igem.org/mediawiki/2017/6/6e/C2V_Design_Header.jpg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-59a5b-rjfwmrih-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div class="column half_size">
+
<div id="vbid-59a5b-rjfwmrih" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/a/ae/1kUP0HHD0vdTZ1KduS1NQm1h4aT5Q3A2EBFqZHFB-5XS-TnvRFhyaQfKfDaF39d6Slwe0PoX7e9sKU9P0g.jpg);"  data-orig-width="3456" data-orig-height="2304" >
−
<h5>What should this page contain?</h5>
+
<!-- VIDEO TEMPLATE -->
−
<ul>
+
−
<li>Explanation of the engineering principles your team used in your design</li>
+
−
<li>Discussion of the design iterations your team went through</li>
+
−
<li>Experimental plan to test your designs</li>
+
−
</ul>
+
  
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 
</div>
 
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="vbid-59a5b-ejch24qi" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">DESIGN</h3>
  
−
<div class="column half_size">
 
−
<h5>Inspiration</h5>
 
−
<ul>
 
−
<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
 
−
<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
 
−
<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
 
−
</ul>
 
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h1 id="vbid-59a5b-dnp8mtfr" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Our circuit utilizes an arsenic regulatory protein (Arsr) and the green fluorescent protein (GFP) gene so that when Plasmid-containing cells are exposed to arsenic ions, gfp is produced, making the ticket glow under a blue or ultraviolet light. <br></h1>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- PROMO END -->
 +
 +
 +
 +
 +
<!-- SLIDESHOWS START -->
 +
<div  id="vbid-6443b981-vyabf59e"  class="master item-box  gallery-box style-6443b981-vw7knopy      fill-height  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-6443b981-vw7knopy" data-preview-styleid='style-6443b981-vw7knopy' data-preset-type-id="SLIDESHOWS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper slideshows-wrapper ">
 +
 +
<div  class="sub container style-6443b981-vw7knopy content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-6443b981-vw7knopy" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-6443b981-vyabf59e" data-preset-type-id="SLIDESHOWS" data-preview-style="style-6443b981-vw7knopy" data-style="style-89a8e-bbtqrmxrkr" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="6"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-6443b981-7adtsohx"  class="sub item-box  page-box style-6443b981-vw7knopy          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6443b981-vw7knopy" data-preview-styleid='style-6443b981-vw7knopy' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-6443b981-7adtsohx" data-preview-style="style-6443b981-vw7knopy" data-style="style-89a8e-q4q4gr5nhr" data-orig-thumb-height="324" data-orig-thumb-width="337" data-vbid="vbid-6443b981-7adtsohx" data-bgimg="https://static.igem.org/mediawiki/2017/1/12/Synthetic_biology.png">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-6443b981-sh3v5uxh-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-6443b981-sh3v5uxh" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/1/12/Synthetic_biology.png);"  data-orig-width="337" data-orig-height="324" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-6443b981-osbfzpnv" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >the Thought Process<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- SLIDESHOWS END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-88f5fc9c-gr2ixtpe"  class="master item-box  gallery-box style-88f5fc9c-1i6hnza7    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-88f5fc9c-1i6hnza7" data-preview-styleid='style-88f5fc9c-1i6hnza7' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-88f5fc9c-1i6hnza7 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-88f5fc9c-1i6hnza7" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-88f5fc9c-gr2ixtpe" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-88f5fc9c-1i6hnza7" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-88f5fc9c-0pcncze1"  class="sub item-box  page-box style-88f5fc9c-1i6hnza7          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-88f5fc9c-1i6hnza7" data-preview-styleid='style-88f5fc9c-1i6hnza7' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-88f5fc9c-0pcncze1" data-preview-style="style-88f5fc9c-1i6hnza7" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="525" data-orig-thumb-width="544" data-vbid="vbid-88f5fc9c-0pcncze1" data-bgimg="https://static.igem.org/mediawiki/2017/5/5a/C2V_Design_Ars2plasmidmap.png">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-right" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-88f5fc9c-v22rkfln-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-88f5fc9c-v22rkfln" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/5/5a/C2V_Design_Ars2plasmidmap.png);"  data-orig-width="544" data-orig-height="525" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="element-287f1dcd2868fb6" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >design</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-88f5fc9c-t14shpss" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span id="docs-internal-guid-1b7da29c-f973-ba3d-a6ea-b5f9a7774632"><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Cadets2Vets utilized literature searches and the <a data-cke-saved-href="http://parts.igem.org/Catalog" href="http://parts.igem.org/Catalog">iGEM Registry of Standard Biologic</a><span style="color:#000000;"><a data-cke-saved-href="http://parts.igem.org/Catalog" href="http://parts.igem.org/Catalog">al Parts</a> to identify the appropriate parts and sequences to use for the arsenic circuit. We knew we needed to have unregulated expression of ArsR and that ArsR needed to control expression of the GFP reporter. We found constitutive and regulated promoters to fit our needs.</span></span></span></p><p><br></p><p><span><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;"><span style="color:#000000;">Then we used DNA sequence analysis software, like Genome Compiler, to assemble a virtual sequence of what we needed. We used this software to add in additional features like the BioBrick Prefix and Suffix, as well as make predictions regarding the cloning steps we would take to create the arsenic circuit. The sequences of the Parts we selected can be found </span><a data-cke-saved-href="https://2017.igem.org/Team:Cadets2Vets/Parts" href="https://2017.igem.org/Team:Cadets2Vets/Parts"><span style="color:#0000CD;">here</span></a><span style="color:#000000;">.</span></span></span></p><p><br></p><p><span><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;"><span style="color:#000000;">To test the plasmids, we would use a variety of assays that would assess whether the correct proteins were being generated and whether the proteins were functioning as expected.</span></span></span>​</p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-34ff8b6b-xfnfbe56"  class="master item-box  gallery-box style-34ff8b6b-n0lxhwvu    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-34ff8b6b-n0lxhwvu" data-preview-styleid='style-34ff8b6b-n0lxhwvu' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-34ff8b6b-n0lxhwvu content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-34ff8b6b-n0lxhwvu" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-34ff8b6b-xfnfbe56" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-34ff8b6b-n0lxhwvu" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-34ff8b6b-qt50pjez"  class="sub item-box  page-box style-34ff8b6b-n0lxhwvu          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-34ff8b6b-n0lxhwvu" data-preview-styleid='style-34ff8b6b-n0lxhwvu' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-34ff8b6b-qt50pjez" data-preview-style="style-34ff8b6b-n0lxhwvu" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="467" data-orig-thumb-width="443" data-vbid="vbid-34ff8b6b-qt50pjez" data-bgimg="https://static.igem.org/mediawiki/2017/d/df/C2V_Design_Build_dnagel.png">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-left" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-34ff8b6b-adi8ojmf-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-34ff8b6b-adi8ojmf" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/d/df/C2V_Design_Build_dnagel.png);"  data-orig-width="443" data-orig-height="467" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-34ff8b6b-wmxuvsaa" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >build</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-34ff8b6b-rhpe0gbn" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span id="docs-internal-guid-1b7da29c-f973-ba3d-a6ea-b5f9a7774632"><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">​</span></span><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;"></span><span style="letter-spacing: 1px; background-color: transparent; font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">The creation of the circuit was achieved by obtaining synthesized DNA from Integrated DNA Technologies and using enzymes and competent </span><span style="letter-spacing: 1px; background-color: transparent; font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="letter-spacing: 1px; background-color: transparent; font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"> cells obtained from New England Biolabs’ BioBrick Assembly Kit. DNA was cut using restriction enzymes and then linked to a vector backbone or to another piece of DNA using T4 ligase. The plasmid was then transformed into bacteria and then the plasmid DNA was isolated. We screened for the correct plasmid by using restriction enzyme digest and seeing that the resulting fragment matched our predictions. </span><span><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;"><span style="color:#000000;"></span></span></span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-53331cba-xfnfbe56"  class="master item-box  gallery-box style-53331cba-n0lxhwvu    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-53331cba-n0lxhwvu" data-preview-styleid='style-53331cba-n0lxhwvu' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-53331cba-n0lxhwvu content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-53331cba-n0lxhwvu" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-53331cba-xfnfbe56" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-53331cba-n0lxhwvu" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-53331cba-qt50pjez"  class="sub item-box  page-box style-53331cba-n0lxhwvu          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-53331cba-n0lxhwvu" data-preview-styleid='style-53331cba-n0lxhwvu' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-53331cba-qt50pjez" data-preview-style="style-53331cba-n0lxhwvu" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="2304" data-orig-thumb-width="3456" data-vbid="vbid-53331cba-qt50pjez" data-bgimg="https://static.igem.org/mediawiki/2017/6/6e/C2V_Design_Header.jpg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-right" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-53331cba-adi8ojmf-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-53331cba-adi8ojmf" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/6/6e/C2V_Design_Header.jpg);"  data-orig-width="3456" data-orig-height="2304" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-53331cba-wmxuvsaa" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >test</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-53331cba-rhpe0gbn" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span style="font-size:16px;">In general, the strategy was to:</span></p><ol><li><span style="font-size:16px;">determine that proteins of the correct size were being produced</span></li><li><span style="font-size:16px;">determine that the GFP was fluorescing when we expected it to fluoresce</span></li><li><span style="font-size:16px;">optimize the assay conditions for the in vitro transcription/translation protocol</span></li><li><span style="font-size:16px;">optimize transfer of the assay to paper tickets. ​</span></li></ol><p dir="ltr"><span style="font-size:16px;">Our initial assessments of the plasmids were performed by monitoring the effect of a range of arsenic concentrations on the arsenic circuit being expressed by live E. coli cells, examining GFP fluorescence using a Light Cycler as a spectrophotometer, and looking at protein expression using a protein gel.</span></p><p dir="ltr"><br></p><p dir="ltr"><span style="font-size:16px;">As we move forward in testing our circuit, we hope to identify the concentration range of arsenic that our circuit can report; the working conditions that the circuit will function, such as temperature and incubation time of the assay; and the specificity of the circuit. Additionally, our team is developing a portable imaging system using technology made by ECBC.</span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-bd0b19d9-y96rikgz"  class="master item-box  gallery-box style-bd0b19d9-rkrbtjpe    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-bd0b19d9-rkrbtjpe" data-preview-styleid='style-bd0b19d9-rkrbtjpe' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-bd0b19d9-rkrbtjpe content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-bd0b19d9-rkrbtjpe" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-bd0b19d9-y96rikgz" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-bd0b19d9-rkrbtjpe" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-bd0b19d9-bpaqly4c"  class="sub item-box  page-box style-bd0b19d9-rkrbtjpe          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-bd0b19d9-rkrbtjpe" data-preview-styleid='style-bd0b19d9-rkrbtjpe' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-bd0b19d9-bpaqly4c" data-preview-style="style-bd0b19d9-rkrbtjpe" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="3024" data-orig-thumb-width="4032" data-vbid="vbid-bd0b19d9-bpaqly4c" data-bgimg="https://static.igem.org/mediawiki/2017/8/87/C2V_Description_ArsLevels.jpg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-left" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-bd0b19d9-2h5bof2o-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-bd0b19d9-2h5bof2o" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/a/ab/C2V_Design_Cellfreeextract.jpg);"  data-orig-width="4032" data-orig-height="3024" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-bd0b19d9-fcd5o48r" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >cell-free lysate</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-bd0b19d9-x6fcw58a" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span id="docs-internal-guid-1b7da29c-f973-ba3d-a6ea-b5f9a7774632"><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">​</span></span><span style="font-size:16px;">The use of cell-free extracts is important to the progress and future development of the arsenic circuit, and other similarly styled circuits.</span></p><p dir="ltr"><br></p><p dir="ltr"><span style="font-size:16px;">The first advantage is that the use of cell-free extracts increases the shelf-life of the assay. We expect to be able to lyophilize the enzymes and proteins used in the arsenic circuit, so we do not have to be concerned with keeping bacteria cells alive and healthy. </span></p><p dir="ltr"><br></p><p dir="ltr"><span style="font-size:16px;">The second advantage is that there will be no chance of accidentally releasing a modified bacteria to the environment. Although there is a low risk that the bacteria we use would adversely affect the environment, due to the required selective pressure needed to maintain the plasmid and the relative safety of the produced proteins, we will maintain laboratory safety standards and laws that prohibit the release of genetically modified organisms.</span></p><p dir="ltr"><br></p><p dir="ltr"><span style="font-size:16px;">The third advantage is that we expect to be able to detect a broader range of arsenic using a cell-free system. Using live cells requires arsenic to be able to transport into the cell and interact with the necessary proteins. Having the proteins readily available in solution should reduce the barrier of arsenic accessing ArsR. </span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- FOOTERS START -->
 +
<div  id="vbid-59a5b-mskg6fxi"  class="master item-box  footer-box style-59a5b-lbaqmrpi injected        " data-holder-type="footer"" data-holder-type="footer"  data-child-type="ORIGIN"  data-styleid="style-59a5b-lbaqmrpi" data-preview-styleid='style-59a5b-lbaqmrpi' data-preset-type-id="FOOTERS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="footer-wrapper item-wrapper footers-wrapper ">
 +
 +
<div class="item-content leaf middle_layout footer content shrinker-parent" data-self="vbid-59a5b-mskg6fxi" data-preview-style="style-59a5b-lbaqmrpi" data-style="style-f785e-jp5ywgaa0t" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-mskg6fxi" data-bgimg="">
 +
<div  class="preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
 +
<div  id="no-image" data-title='click to Add'  class="background-div  normal preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="PREVIEW_IMAGE" style="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
</div>
 +
<div class="helper-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="item-details preview-content-wrapper  middle">
 +
<div class="preview-content-holder shrinker-content">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
<!-- TITLE TEMPLATE -->
 +
 +
<div class="preview-title-holder removable-parent">
 +
 +
<h2 id="vbid-59a5b-xinhfnbg" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Follow us on Social Media!</h2>
 +
 +
</div>
 +
<br />
 +
 +
<!-- SUBTITLE TEMPLATE -->
 +
 +
<div class="preview-subtitle-holder removable-parent">
 +
 +
<h3 id="vbid-59a5b-novarlkx" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, Tacoma, WA 98402</h3>
 +
 +
</div>
 +
<br />
 +
 +
<!-- BODY TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
<!-- PRICE TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
<!-- FORM TEMPLATE -->
 +
<div class="preview-form">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
<!-- LINKS TEMPLATE -->
 +
<div class="preview-item-links ">
 +
<div class="preview-links-wrapper">
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
<!-- SOCIAL TEMPLATE -->
 +
 +
<div class="preview-social-wrapper removable-parent"  >
 +
<div id="vbid-59a5b-xmkwjkjw" class="preview-element preview-social-holder magic-circle-holder" data-menu-name="PREVIEW_SOCIAL" data-theme="7" >
 +
 +
<div id="FACEBOOK" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="https://static.igem.org/mediawiki/2017/a/a4/FacebookImage.png">
 +
<a class='social-link-url' href="https://www.facebook.com/RAINIncubator" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/a/a4/FacebookImage.png" ></a>
 +
</div>
 +
 +
<div id="TWITTER" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="https://static.igem.org/mediawiki/2017/5/59/TwitterImage.png">
 +
<a class='social-link-url' href="https://twitter.com/RAINincubator" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/5/59/TwitterImage.png" ></a>
 +
</div>
 +
 +
<div id="INSTAGRAM" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="INSTAGRAM" data-img-url="https://static.igem.org/mediawiki/2017/8/86/InstagramImage.png">
 +
<a class='social-link-url' href="https://www.linkedin.com/in/rain-network" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/8/86/InstagramImage.png" ></a>
 +
</div>
 +
 +
<div id="YOUTUBE" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="YOUTUBE" data-img-url="https://static.igem.org/mediawiki/2017/3/34/YoutubeImage.png">
 +
<a class='social-link-url' href="https://www.youtube.com/channel/UCuVc4cKxuUzpjZfywBgoT9w" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/3/34/YoutubeImage.png" ></a>
 +
</div>
 +
 +
<div id="GOOGLE_PLUS" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="GOOGLE_PLUS" data-img-url="https://static.igem.org/mediawiki/2017/f/fa/GooglePlusImage.png">
 +
<a class='social-link-url' href="https://plus.google.com/u/0/+RainincubatorOrg" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/f/fa/GooglePlusImage.png" ></a>
 +
</div>
 +
 +
</div>
 +
</div>
 +
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="middle"></div>
 +
 +
</div>
 +
</div>
 +
<!-- FOOTERS END -->
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="light-box-wrapper  space-layer" style="display:none;">
 +
<div class="light-box-image-holder">
 +
<div class="light-box-image animated">
 +
 +
</div>
 +
<div class="lightbox-text-wrapper ">
 +
<div class="lightbox-text-holder animated">
 +
<div class="lightbox-title"></div>
 +
<div class="lightbox-subtitle"></div>
 +
<div id="paginator" style="display:none;">
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
    <img src="https://static.igem.org/mediawiki/2017/d/db/Arrow1.png" class="download-gallery-btn clickable" style="display: none;">
 +
<img src="https://static.igem.org/mediawiki/2017/9/9e/XImage1.png" class="close-lightbox-btn clickable" style="opacity: 1;">
 +
<img src="https://2017.igem.org/File:Thing5.png" class="lightbox-arrow lightbox-left clickable top-layer" >
 +
<img src="https://2017.igem.org/File:Thing4.png" class="lightbox-arrow lightbox-right clickable top-layer" >
 +
</div>
  
 +
</div>
 +
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lightbox?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/spimeengine?action=raw&ctype=text/javascript"></script>
 +
 +
<!--  SHOPROCKET  ECOMMERCE  -->
 +
<script>
 +
var JSR = jQuery.noConflict();
 +
var $ = jQuery.noConflict();
 +
</script>
 +
 +
<input type="hidden" name="sr-companyid" id="sr-companyid" value="">
 +
</body>
  
 +
 
</html>
 
</html>

Latest revision as of 02:49, 1 November 2017

HOME
TEAM
PROJECT
Description
Design
Experiments
Notebook
Notebook Overview
June Notebook
July Notebook
August Notebook
September Notebook
October Notebook
InterLab
Results
Demonstrate
Improve
Attributions
PARTS
Parts
Basic Parts
Composite Parts
SAFETY
HUMAN PRACTICES
Silver HP
Integrated and Gold
Public Engagement
AWARDS
Hardware
Environmental Project
JUDGING FORM

Design - Home


DESIGN



Our circuit utilizes an arsenic regulatory protein (Arsr) and the green fluorescent protein (GFP) gene so that when Plasmid-containing cells are exposed to arsenic ions, gfp is produced, making the ticket glow under a blue or ultraviolet light.



the Thought Process



design



Cadets2Vets utilized literature searches and the iGEM Registry of Standard Biological Parts to identify the appropriate parts and sequences to use for the arsenic circuit. We knew we needed to have unregulated expression of ArsR and that ArsR needed to control expression of the GFP reporter. We found constitutive and regulated promoters to fit our needs.


Then we used DNA sequence analysis software, like Genome Compiler, to assemble a virtual sequence of what we needed. We used this software to add in additional features like the BioBrick Prefix and Suffix, as well as make predictions regarding the cloning steps we would take to create the arsenic circuit. The sequences of the Parts we selected can be found here.


To test the plasmids, we would use a variety of assays that would assess whether the correct proteins were being generated and whether the proteins were functioning as expected.​



build



​The creation of the circuit was achieved by obtaining synthesized DNA from Integrated DNA Technologies and using enzymes and competent E. coli cells obtained from New England Biolabs’ BioBrick Assembly Kit. DNA was cut using restriction enzymes and then linked to a vector backbone or to another piece of DNA using T4 ligase. The plasmid was then transformed into bacteria and then the plasmid DNA was isolated. We screened for the correct plasmid by using restriction enzyme digest and seeing that the resulting fragment matched our predictions.



test



In general, the strategy was to:

  1. determine that proteins of the correct size were being produced
  2. determine that the GFP was fluorescing when we expected it to fluoresce
  3. optimize the assay conditions for the in vitro transcription/translation protocol
  4. optimize transfer of the assay to paper tickets. ​

Our initial assessments of the plasmids were performed by monitoring the effect of a range of arsenic concentrations on the arsenic circuit being expressed by live E. coli cells, examining GFP fluorescence using a Light Cycler as a spectrophotometer, and looking at protein expression using a protein gel.


As we move forward in testing our circuit, we hope to identify the concentration range of arsenic that our circuit can report; the working conditions that the circuit will function, such as temperature and incubation time of the assay; and the specificity of the circuit. Additionally, our team is developing a portable imaging system using technology made by ECBC.



cell-free lysate



​The use of cell-free extracts is important to the progress and future development of the arsenic circuit, and other similarly styled circuits.


The first advantage is that the use of cell-free extracts increases the shelf-life of the assay. We expect to be able to lyophilize the enzymes and proteins used in the arsenic circuit, so we do not have to be concerned with keeping bacteria cells alive and healthy.


The second advantage is that there will be no chance of accidentally releasing a modified bacteria to the environment. Although there is a low risk that the bacteria we use would adversely affect the environment, due to the required selective pressure needed to maintain the plasmid and the relative safety of the produced proteins, we will maintain laboratory safety standards and laws that prohibit the release of genetically modified organisms.


The third advantage is that we expect to be able to detect a broader range of arsenic using a cell-free system. Using live cells requires arsenic to be able to transport into the cell and interact with the necessary proteins. Having the proteins readily available in solution should reduce the barrier of arsenic accessing ArsR.


Follow us on Social Media!


2304 Jefferson Ave, Tacoma, WA 98402