Difference between revisions of "Team:ICT-Mumbai/Parts"

Line 57: Line 57:
 
     }
 
     }
 
}
 
}
 +
 +
<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">
 +
Our project involves metabolism of ammonia by Escherichia coli to produce a blue-coloured dye,
 +
indigoidine. We had to choose between using a constitutive and an inducible promoter to drive
 +
expression of the genes that we wish to express in E. coli.</p>
 +
 +
<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">Expression from inducible promoters requires addition of an inducing molecule. However, as it would be
 +
cumbersome and tedious to add the inducer to the device that will house the engineered E. coli, and as
 +
it would also contribute to the cost, we decided to use a constitutive promoter to drive gene expression.</p>
 +
 +
<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">We reasoned that the constitutive promoter of choice should have the following two properties: (1) it
 +
should not be a very strong promoter, so as to not lead to any toxicity to the cell, and (2) it should be
 +
active in low-nutrient conditions. Based on these two considerations, the commonly used glycolytic
 +
promoters were ruled out as possible choices.</p>
 +
 +
<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The ychH promoter has been described in literature to be active under low glucose conditions (Ref. 1).
 +
Moreover, it is not a very strong promoter, compared to those frequently employed to express
 +
recombinant proteins in E. coli (Ref. 2). Therefore, the ychH promoter became our promoter of choice.</p>
 +
 +
 +
  
  

Revision as of 02:57, 1 November 2017

ICT-Mumbai 2017