Difference between revisions of "Team:ICT-Mumbai/Safety"

Our host organism

The host organism of our work is the ‘lab pet’ / ‘industrial workhorse’ Escherichia coli. We have used different strains of E. coli for our work – DH5α, BW25113, BL21(DE3) – all of which are categorised under Biosafety Level I (BSL I) and safe to use and handle.

Safe practices in our laboratory

We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal.
Figures
We put on latex gloves and used spatulas to handle ethidium bromide-containing agarose gels. Used gels were put in labelled bags that were handed over to a contractor for disposal by incineration at a municipal facility.

Ensuring safety in our Institute

The Institutional Biosafety Committee of our Institute is responsible for ensuring that safe practices are followed in all laboratories in the Institute. The Committee meets twice a year to review and recommend safety measures. Our team primary PI, Prof. Arvind Lali, is the Member Secretary of the Committee.

Safety regulations in our country

Autoclave facility

Laminar air flow facility