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− | <img id="background-image" src="https://static.igem.org/mediawiki/2017/6/69/WHU-China-child-bg.jpeg" alt=""/>
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− | </div>
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− | <li id="nv0" style="width:200px"><a href="https://2017.igem.org/Team:WHU-China"></a></li>
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− | <a>Project</a>
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− | <ul>
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− | <li id="nv11"><a href="https://2017.igem.org/Team:WHU-China/Overview">Overview</a></li>
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− | <li id="nv12"><a href="https://2017.igem.org/Team:WHU-China/Background">Background</a></li>
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− | <li id="nv13"><a href="https://2017.igem.org/Team:WHU-China/Introduction">Introduction</a></li>
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− | <li id="nv14"><a href="https://2017.igem.org/Team:WHU-China/Designs">Designs</a></li>
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− | <li id="nv15"><a href="https://2017.igem.org/Team:WHU-China/Experiments">Experiments</a></li>
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− | <li id="nv16"><a href="https://2017.igem.org/Team:WHU-China/Results">Results</a></li>
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− | </ul>
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− | </li>
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− | <li id="nv2"><a>Parts</a>
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− | <li id="nv21"><a href="https://2017.igem.org/Team:WHU-China/Description_Part">Description</a></li>
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− | <li id="nv22"><a href="https://2017.igem.org/Team:WHU-China/Basic_Part">Basic</a></li>
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− | <li id="nv23"> <a href="https://2017.igem.org/Team:WHU-China/Composite_Part">Composite</a></li>
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− | <li id="nv3"><a>Interlab</a>
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− | <li id="nv31"><a href="https://2017.igem.org/Team:WHU-China/Interlab">Results</a></li>
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− | <li id="nv32"><a href="https://2017.igem.org/Team:WHU-China/Calibration">Calibration</a></li>
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− | <li id="nv33"><a href="https://2017.igem.org/Team:WHU-China/Cell_Measuremnet">CellMeasuremnet</a></li>
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− | <a>Notebook</a>
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− | <li id="nv41"><a href="https://2017.igem.org/Team:WHU-China/Dry_Lab">DryLab</a></li>
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− | <li id="nv42"><a href="https://2017.igem.org/Team:WHU-China/Wet_Lab">WetLab</a></li>
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− | <a>Model</a>
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− | <li id="nv51"><a href="https://2017.igem.org/Team:WHU-China/Model">Modeling</a></li>
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− | <li id="nv52"><a href="https://2017.igem.org/Team:WHU-China/Hardware">Hardware</a></li>
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− | <a>Human Practice</a>
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− | <li id="nv61"><a href="https://2017.igem.org/Team:WHU-China/HP/Silver">Silver</a></li>
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− | <li id="nv62"><a href="https://2017.igem.org/Team:WHU-China/HP/Gold_Integrated">Gold&Integrated</a></li>
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− | <li id="nv63"><a href="https://2017.igem.org/Team:WHU-China/Engagement">Engagement</a></li>
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− | <li id="nv7"><a>Safety</a>
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− | <li id="nv71"><a href="https://2017.igem.org/Team:WHU-China/Project_Design">ProjectDesign</a></li>
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− | <li id="nv72"><a href="https://2017.igem.org/Team:WHU-China/Lab_Work">LabWork</a></li>
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− | <li id="nv8"><a>Team</a>
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− | <li id="nv81"><a href="https://2017.igem.org/Team:WHU-China/Member">Member</a></li>
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− | <li id="nv82"><a href="https://2017.igem.org/Team:WHU-China/Attributions">Attributions</a> </li>
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− | <li id="nv83"><a href="https://2017.igem.org/Team:WHU-China/Collaborations">Collaborations</a></li>
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− | <li id="nv9"><a href="https://2017.igem.org/Team:WHU-China/Achievement">Achievement</a>
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| + | <div id="contents"> |
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| <h1>Cell measurement protocol</h1> | | <h1>Cell measurement protocol</h1> |
| <ul id="cont"> | | <ul id="cont"> |
− | <h2>1. Transform E.Coli DH5α with these following plasmids:</h2> | + | |
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| + | <h2>1. Transform <i>E. coli</i> DH5α with these following plasmids:</h2> |
| <h3> a) Positive Control</h3> | | <h3> a) Positive Control</h3> |
| <h3> b) Negative Control</h3> | | <h3> b) Negative Control</h3> |
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| <h3> h) Test Device 6</h3> | | <h3> h) Test Device 6</h3> |
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− | <h2>2. Pick 2 colonies from each of plate and inoculate it on 5 mL LB medium + Chloramphenicol. Grow the cells overnight (16 hours) at 37°C and 220 rpm.</h2> | + | <h2>2. Pick 2 colonies from each plate and inoculate it on 5 mL LB medium + Chloramphenicol. Grow the cells overnight (16 hours) at 37°C and 220 rpm.</h2> |
| <h2>3. Cell growth, sampling, and assay</h2> | | <h2>3. Cell growth, sampling, and assay</h2> |
| <h3> a) Set your instrument to read OD600 (as OD calibration setting)</h3> | | <h3> a) Set your instrument to read OD600 (as OD calibration setting)</h3> |
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| <h3> g) Record data in the notebook</h3> | | <h3> g) Record data in the notebook</h3> |
| <h3> h) Import data into Excel (cell measurement tab) Sheet_1 provided</h3> | | <h3> h) Import data into Excel (cell measurement tab) Sheet_1 provided</h3> |
− | | + | <h2></h2><h2></h2> |
| </ul> | | </ul> |
| </div> | | </div> |