Difference between revisions of "Team:Cardiff Wales/compositeparts"

 
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<h1> Composite Parts </h1>
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<h1> Composite Parts generated in our Project</h1>
 
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<br><br>
 
<table style="width:90%">
 
<table style="width:90%">
 
   <tr>
 
   <tr>
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     <th>Promotor </th>
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     <th>Registry link</th>
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     <th>Coding sequence</th>  
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    <th>Promoter</th>
 +
     <th>Coding Sequence</th>
 
     <th>Terminator</th>
 
     <th>Terminator</th>
 
     <th>Plasmid</th>
 
     <th>Plasmid</th>
 
     <th>Summary</th>
 
     <th>Summary</th>
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     <th>Approximate Length</th>
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     <th>Length</th>
 
      
 
      
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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     <td>35S</td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2404013:Experience">BBa_K2404013</a></td>
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     <td>TSH</td>
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    <td><a href="http://parts.igem.org/Part:BBa_P10003">35S-OTMV</a></td>
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     <td>NosT</td>
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     <td>Luc+</td>
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     <td>pGB-A2</td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10401">NosT</a></td>
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     <td>A constitutive promoter with our TSH antagonist <b>without</b> the his tags for purification</td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10501">pGB-A1</a></td>
 +
     <td>For constitutive expression of Luc+</td>
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    <td>3386bp</td>
 +
 
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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     <td>35S</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2404014">BBa_K2404014</a></td>
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     <td>TSHH</td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10000">LexA</a></td>
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     <td>NosT</td>
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     <td>Luc+</td>
−
     <td>pGB-A2</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10401">NosT</a></td>
−
     <td>A constitutive promoter with our TSH antagonist <b>with</b> the his tags for purification</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10501">pGB-A1</a></td>
 +
     <td>For inducible expression of Luc+ in presence of the <a href="http://parts.igem.org/Part:BBa_P10300">XVE</a> protein and estradiol</td>
 +
    <td>2287bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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     <td>35S</td>
+
    <td><a href="http://parts.igem.org/Part:BBa_K2404016">BBa_K2404016</a></td>
−
     <td>Luc+</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10000">LexA</a></td>
−
     <td>NosT</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_K2404001">TSHH</a></td>
−
     <td>pGB-A2</td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10401">NosT</a></td>
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     <td>A constitutive promoter with Luc+ as a coding sequence to analyse expression levels</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 +
     <td>For inducible expression of <a href="http://parts.igem.org/Part:BBa_K2404001">TSHH</a> in presence of the <a href="http://parts.igem.org/Part:BBa_P10300">XVE</a> protein and estradiol</td>
 +
    <td>1451bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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     <td>35S</td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2404018">BBa_K2404018</a></td>
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     <td>Lucx5</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_K2404002">PDF1.2</a></td>
−
     <td>NosT</td>
+
     <td><a href="http://parts.igem.org/Part:BBa_K2404001">TSHH</a></td>
−
     <td>pGB-A2</td>
+
<td></td>
 +
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 +
    <td>For inducible expression of <a href="http://parts.igem.org/Part:BBa_K2404001">TSHH</a> in presence of the plant hormone <a href="https://en.wikipedia.org/wiki/Jasmonic_acid">methyl jasmonate</a></td>
 +
    <td>1339bp</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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     <td>35S</td>
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        <td><a href="http://parts.igem.org/Part:BBa_K2404020">BBa_K2404020</a></td>
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     <td>XVE</td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2404003">PR2</a></td>
−
     <td>NosT</td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2404001">TSHH</a></td>
−
     <td>pGB-A2</td>
+
<td></td>
 +
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 +
    <td>For inducible expression of <a href="http://parts.igem.org/Part:BBa_K2404001">TSHH</a> in presence of the plant hormone <a href="https://en.wikipedia.org/wiki/Salicylic_acid">salicyclic acid</a></td>
 +
    <td>1336bp</td>
 +
  </tr>
 +
<tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2404022">BBa_K2404022</a></td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2404004">GST6</a></td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2404001">TSHH</a></td>
 +
<td><a href="http://parts.igem.org/Part:BBa_P10401">NosT</a></td>
 +
     <td><a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a></td>
 +
    <td>For inducible expression of <a href="http://parts.igem.org/Part:BBa_K2404001">TSHH</a> in presence of the plant hormone <a href="https://en.wikipedia.org/wiki/Salicylic_acid">salicyclic acid</a></td>
 +
    <td>1608bp</td>
 
   </tr>
 
   </tr>
−
</table>
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  </table>
 +
<br><br>Unfortunately we did not add part information to our <a href="https://igem.org/2017_Judging_Form?id=2404">Judging Form</a>, but our contributions are outlined in the table above.
 
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Latest revision as of 08:58, 1 November 2017




Composite Parts generated in our Project








Registry link Promoter Coding Sequence Terminator Plasmid Summary Length
BBa_K2404013 35S-OTMV Luc+ NosT pGB-A1 For constitutive expression of Luc+ 3386bp
BBa_K2404014 LexA Luc+ NosT pGB-A1 For inducible expression of Luc+ in presence of the XVE protein and estradiol 2287bp
BBa_K2404016 LexA TSHH NosT pGB-A2 For inducible expression of TSHH in presence of the XVE protein and estradiol 1451bp
BBa_K2404018 PDF1.2 TSHH pGB-A2 For inducible expression of TSHH in presence of the plant hormone methyl jasmonate 1339bp
BBa_K2404020 PR2 TSHH pGB-A2 For inducible expression of TSHH in presence of the plant hormone salicyclic acid 1336bp
BBa_K2404022 GST6 TSHH NosT pGB-A2 For inducible expression of TSHH in presence of the plant hormone salicyclic acid 1608bp


Unfortunately we did not add part information to our Judging Form, but our contributions are outlined in the table above.